LOCUS       sequence-48918-e        1360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1360)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: OXSM
FEATURES             Location/Qualifiers
     source          1..1360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1115..1126
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gattgcatag gcgagttgtc attacaggca ttggcttagt gactcctctt
      121 ggtgttggaa ctcacctggt ttgggatcgt cttatcggag gagagagtgg aattgtttca
      181 ctggttggtg aagagtataa gagtatccct tgcagtgttg ctgcttatgt gccaagaggt
      241 agtgatgaag gtcagttcaa tgaacaaaac tttgtgtcca aatcagatat caagtccatg
      301 tcttctccca ccatcatggc cattggggct gcagaattag ccatgaagga ttctggctgg
      361 catcctcagt cagaagctga tcaagtggct actggtgttg caattggcat gggaatgatt
      421 cctcttgaag ttgtttctga aactgctttg aattttcaga caaaaggtta caataaagtt
      481 agcccatttt ttgtccctaa gattctggtc aatatggcag caggccaggt cagcattcga
      541 tataaactca agggcccaaa tcatgcagta tccacagcct gtaccacagg agctcatgct
      601 gtgggagact catttagatt tatagcccat ggtgatgctg atgtgatggt ggctggaggt
      661 acagattctt gtattagccc tttatctctt gctgggtttt ccagagcccg ggctctgagc
      721 acaaactcag atcccaagtt ggcatgtcga ccatttcatc caaagagaga tggttttgta
      781 atgggagaag gtgcagctgt gctggtgctg gaagaatatg aacatgctgt tcaaagaaga
      841 gcccggatct atgcagaagt tttgggctat ggactctcag gtgatgctgg tcacataact
      901 gcccctgatc ctgaaggaga aggtgcctta aggtgtatgg ctgctgcttt aaaagatgca
      961 ggtgtgcagc ctgaggagat atcctatatc aatgcacatg ctacttccac accattggga
     1021 gatgctgctg aaaacaaagc tatcaaacat ctcttcaaag accatgcata tgcccttgca
     1081 gtttcctcaa ctaagggagc aacaggacat ctgctgggag ctgcaggggc agtcgaggca
     1141 gcttttacca cattagcttg ttattatcaa aaactaccac ctactttaaa cctggattgt
     1201 tcggaaccag aatttgatct caactatgtt ccactaaagg cacaggaatg gaaaactgag
     1261 aaaagattta ttggcctcac caattccttt ggttttggtg gtactaatgc aacactttgt
     1321 attgctggac tgtagcagta aaggtggata cggatccgaa
//
