LOCUS       sequence-489209-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the 
            Synaptosomal-Associated Protein (SNAP-25) with K40E mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, 
            Chung WK, Monteggia LM, Brunger AT, Kavalali ET
  TITLE     Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated 
            Encephalopathies.
  JOURNAL   Neuron. 2021 Jan 6;109(1):59-72.e5. doi: 
            10.1016/j.neuron.2020.10.012. Epub 2020 Nov 3.
  PUBMED    33147442
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.10.012"; journalName: "Neuron"; date: 
            "2021-01-6- 6"; volume: "109"; issue: "1"; pages: "59-72.e5"
COMMENT     Sequence Label: pFUW-SNAP25B-K40E
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(791..811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt
       61 cgactctaga ggatcgaatt catggccgag gacgcagaca tgcgcaatga gctggaggag
      121 atgcagcgaa gggctgacca gttggctgat gagtcgctgg aaagcacccg tcgtatgctg
      181 caactggttg aagagagtga agatgctggt atcaggactt tggttatgtt ggatgaacaa
      241 ggagaacaac tggaacgcat tgaggaaggg atggaccaaa tcaataagga catgaaagaa
      301 gcagaaaaga atttgacgga cctaggaaaa ttctgcgggc tttgtgtgtg tccctgtaac
      361 aagcttaaat caagtgatgc ttacaaaaaa gcctggggca ataatcagga cggagtggtg
      421 gccagccagc ctgctcgtgt agtggacgaa cgggagcaga tggccatcag tggcggcttc
      481 atccgcaggg taacaaatga tgcccgagaa aatgaaatgg atgaaaacct agagcaggtg
      541 agcggcatca tcgggaacct ccgtcacatg gccctggata tgggcaatga gatcgataca
      601 cagaatcgcc agatcgacag gatcatggag aaggctgatt ccaacaaaac cagaattgat
      661 gaggccaacc aacgtgcaac aaagatgctg ggaagtggtt aaggatccgt taacaattcg
      721 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
      781 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      841 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tgg
//
