LOCUS       sequence-489211-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the 
            Synaptosomal-Associated Protein (SNAP-25) with L50S mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, 
            Chung WK, Monteggia LM, Brunger AT, Kavalali ET
  TITLE     Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated 
            Encephalopathies.
  JOURNAL   Neuron. 2021 Jan 6;109(1):59-72.e5. doi: 
            10.1016/j.neuron.2020.10.012. Epub 2020 Nov 3.
  PUBMED    33147442
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.10.012"; journalName: "Neuron"; date: 
            "2021-01-6- 6"; volume: "109"; issue: "1"; pages: "59-72.e5"
COMMENT     Sequence Label: pFUW-SNAP25B-L50S
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(790..810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
       61 gactctagag gatcgaattc atggccgagg acgcagacat gcgcaatgag ctggaggaga
      121 tgcagcgaag ggctgaccag ttggctgatg agtcgctgga aagcacccgt cgtatgctgc
      181 aactggttga agagagtaaa gatgctggta tcaggacttt ggttatgtcg gatgaacaag
      241 gagaacaact ggaacgcatt gaggaaggga tggaccaaat caataaggac atgaaagaag
      301 cagaaaagaa tttgacggac ctaggaaaat tctgcgggct ttgtgtgtgt ccctgtaaca
      361 agcttaaatc aagtgatgct tacaaaaaag cctggggcaa taatcaggac ggagtggtgg
      421 ccagccagcc tgctcgtgta gtggacgaac gggagcagat ggccatcagt ggcggcttca
      481 tccgcagggt aacaaatgat gcccgagaaa atgaaatgga tgaaaaccta gagcaggtga
      541 gcggcatcat cgggaacctc cgtcacatgg ccctggatat gggcaatgag atcgatacac
      601 agaatcgcca gatcgacagg atcatggaga aggctgattc caacaaaacc agaattgatg
      661 aggccaacca acgtgcaaca aagatgctgg gaagtggtta aggatccgtt aacaattcga
      721 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
      781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
      841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct gg
//
