LOCUS       sequence-489216-        9124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-WT
FEATURES             Location/Qualifiers
     source          1..9124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2565..2964
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3612..3631
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    3814..4402
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3867..3887)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4285..4296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4405..4421)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4406..4422)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4927..5107
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5133..5150)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5139..5363
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5409..5837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5496..5515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5706..5727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5846..5866)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5851..6180
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6031..6166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6093..6112
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6228..6275
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6294..6668
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6798..6919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6835..6854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6889..6908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6968..6984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6968..6984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6981..7003)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6992..7008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7016..7022,7023..7040,7041..7046))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7061..7082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7199..7216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7370..7958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7450..7469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8129..8920,8921..8989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8752..8771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8990..9094)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaagggtg cagcggcctc cgcgccgggt
     2581 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     2641 gcgcaggagc gttcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     2701 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     2761 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     2821 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     2881 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     2941 ggatcgctgt gatcgtcact tggtgagttg cgggctgctg ggctggccgg ggctttcgtg
     3001 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     3061 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcacaaa atggcggctg
     3121 ttcccgagtc ttgaatggaa gacgcttgta aggcgggctg tgaggtcgtt gaaacaaggt
     3181 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     3241 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     3301 tgactggaga actcgggttt gtcgtctggt tgcgggggcg gcagttatgc ggtgccgttg
     3361 ggcagtgcac ccgtaccttt gggagcgcgc gcctcgtcgt gtcgtgacgt cacccgttct
     3421 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     3481 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     3541 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     3601 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     3661 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     3721 ttagactagt aaagcttctg caggtcgact ctagaggatc gaattcttcg aagctagcgg
     3781 atccgttaac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     3841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3961 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4021 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4081 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4141 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4201 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4261 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4321 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4381 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     4441 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4501 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4561 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4621 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4681 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     4741 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     4801 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     4861 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     4921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5101 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5161 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5221 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5281 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5341 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5401 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5461 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5521 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5581 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5641 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5701 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5761 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5821 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5881 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6001 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6061 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6121 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6181 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     6241 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     6301 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     6361 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     6421 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     6481 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     6541 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     6601 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     6661 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6721 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6781 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6901 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6961 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7021 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7081 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7141 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7201 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7261 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7321 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7381 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7441 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7501 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7561 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7621 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7681 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7741 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7801 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7861 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9121 acac
//
