LOCUS       sequence-489221-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with E78A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-E78A
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(521..541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt
       61 cgactctaga ggatcgaatt catgtcggct accgctgcca ccgtcccgcc tgccgccccg
      121 gccggcgagg gtggcccccc tgcacctcct ccaaatctta ccagtaacag gagactgcag
      181 cagacccagg cccaggtgga tgaggtggtg gacatcatga gggtgaatgt ggacaaggtc
      241 ctggagcgag accagaagct atcggaactg gatgatcgcg cagatgccct ccaggcaggg
      301 gcctcccagt ttgcaacaag tgcagccaag ctcaagcgca aatactggtg gaaaaacctc
      361 aagatgatga tcatcttggg agtgatttgc gccatcatcc tcatcatcat catcgtttac
      421 ttcagcactt aaggatccgt taacaattcg atatcaagct tatcgataat caacctctgg
      481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
      541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
      601 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
      661 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
      721 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
      781 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
      841 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
      901 cctgg
//
