LOCUS       sequence-489225-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with G73W mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-G73W
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(528..548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(946..957)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctagtaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc
       61 tgcaggtcga ctctagagga tcgaattcat gtcggctacc gctgccaccg tcccgcctgc
      121 cgccccggcc ggcgagggtg gcccccctgc acctcctcca aatcttacca gtaacaggag
      181 actgcagcag acccaggccc aggtggatga ggtggtggac atcatgaggg tgaatgtgga
      241 caaggtcctg gagcgagacc agaagctatc ggaactggat gatcgcgcag atgccctcca
      301 ggcatgggcc tcccagtttg aaacaagtgc agccaagctc aagcgcaaat actggtggaa
      361 aaacctcaag atgatgatca tcttgggagt gatttgcgcc atcatcctca tcatcatcat
      421 cgtttacttc agcacttaag gatccgttaa caattcgata tcaagcttat cgataatcaa
      481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
      541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
      601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
      661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
      721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
      781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
      841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
      901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
      961 gcggaccttc cttcccgcgg
//
