LOCUS       sequence-489229-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with R56X mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-R56X
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    294..882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(347..367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(765..776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(885..901)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(886..902)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gactagtaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg
       61 gctgcaggtc gactctagag gatcgaattc atgtcggcta ccgctgccac cgtcccgcct
      121 gccgccccgg ccggcgaggg tggcccccct gcacctcctc caaatcttac cagtaacagg
      181 agactgcagc agacccaggc ccaggtggat gaggtggtgg acatcatgag ggtgaatgtg
      241 gacaaggtcc tggagtaagg atccgttaac aattcgatat caagcttatc gataatcaac
      301 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      361 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      421 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      481 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
      541 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
      601 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
      661 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
      721 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
      781 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
      841 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
      901 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
      961 gtgcctggct agaagcacaa gaggaggagg gaggtgggtt ttccagtcac acctc
//
