LOCUS       sequence-489233-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with V43del deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-V43del
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(508..528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga
       61 ggatcgaatt catgtcggct accgctgcca ccgtcccgcc tgccgccccg gccggcgagg
      121 gtggcccccc tgcacctcct ccaaatctta ccagtaacag gagactgcag cagacccagg
      181 cccaggtgga tgaggtggac atcatgaggg tgaatgtgga caaggtcctg gagcgagacc
      241 agaagctatc ggaactggat gatcgcgcag atgccctcca ggcaggggcc tcccagtttg
      301 aaacaagtgc agccaagctc aagcgcaaat actggtggaa aaacctcaag atgatgatca
      361 tcttgggagt gatttgcgcc atcatcctca tcatcatcat cgtttacttc agcacttaag
      421 gatccgttaa caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
      481 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
      541 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
      601 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
      661 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
      721 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
      781 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
      841 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct gg
//
