LOCUS       sequence-489235-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with I45del deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-I45del
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(506..526)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ttctggccgt ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagg
       61 atcgaattca tgtcggctac cgctgccacc gtcccgcctg ccgccccggc cggcgagggt
      121 ggcccccctg cacctcctcc aaatcttacc agtaacagga gactgcagca gacccaggcc
      181 caggtggatg aggtggtgga catgagggtg aatgtggaca aggtcctgga gcgagaccag
      241 aagctatcgg aactggatga tcgcgcagat gccctccagg caggggcctc ccagtttgaa
      301 acaagtgcag ccaagctcaa gcgcaaatac tggtggaaaa acctcaagat gatgatcatc
      361 ttgggagtga tttgcgccat catcctcatc atcatcatcg tttacttcag cacttaagga
      421 tccgttaaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
      481 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
      541 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
      601 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
      661 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
      721 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
      781 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
      841 cggggaaatc atcgtccttt
//
