LOCUS       sequence-489244-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggyBac vector containing the UQCRQ coding sequences, modified 
            with silent mutations to allow for PCR-based analysis, along with 
            endogenous promoter and UTRs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL378
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1403..1991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1509..1520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1918..1938
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2016..2618)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2102..2122)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2599..2618
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2630..3130)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2693..2711)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     repeat_region   7527..7561
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8052..8068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8052..8068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8065..8087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8076..8092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8100..8106,8107..8124,8125..8130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8145..8166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8283..8300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8454..9042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8534..8553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9213..10004,10005..10073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9836..9855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgttgga ttaagatgtt cgctaggtgc ttgctgaaac ttctaaggca
     3181 tgtggaaaag ctaaccaatt attcatgacg atactcagat ctgctggtat gcaacttatg
     3241 gtattaggtt tactcttcct ctcaatttat cctcacggcc ttttccactt ccaggtgttt
     3301 acaattgtaa ttattcgctg catgttaatt tgcgaattta caaatttggg tgagaaagtc
     3361 acaattggca tagaagttcc agaaagcaga aaaccattag gtctctcagt cctcccaggt
     3421 ggctgggtac agaatacgta cttggagtac tggaattgta tttgcgatgt tcttaaaaga
     3481 ggacaccgcc tttaaccttg acttattttt aaatgtaaca tactgatgtc cttactttgg
     3541 tctgcacact catctataca tattttggtt gaaaggtttt gggatgtttg cagatgctat
     3601 aaaataaatg aacgttttat tattattcat ccaaatggtg gtagaatttg ccacaatatt
     3661 tctgtcctta cctgtatttc tgttcttgcc ttggagctga gaccaggcta gacattatgg
     3721 tttaaaaaaa tgaggcaaag gagcaccatg aaaagctgaa atgaggggat attcagcgcc
     3781 accccgaagg agactatgct ggaagagtca gcctacactc atagtgcaag atacgaagcc
     3841 agtttgtgca gtacctacat gagaaaacta aggtatgtct gcaaaataca agttgactgc
     3901 ctgccgagcc tccgtggtcc gtaagtagtg ttataaaaca cagccaactg ttgaatgaga
     3961 acttagtcct agataaaata tggccagcaa ttccattaac aaaacactga caagacacct
     4021 cagggcgagc tgtacattga caacagcatc gtcaagcaga atgcacttca gggaaaacat
     4081 aataaggcct ctcacccgac tttccttgct tctcacgtgg ggagaagaaa gaactggggc
     4141 atcgtgattc cccccacccc acgcaaacca tctggggagg acgctggctg ctgtcgaggc
     4201 ggcctcagca ttcatcaatt gacgattatt tgattatttt tgtggctgaa actgacgaaa
     4261 cgtttgtatt atctcttggg aacgttaaca gcctgccagg ggactctaaa acagagtcca
     4321 agagaagcct tcgatatgtg tccctgattt tggtgctggg gtcgctcatg gcctgaaaat
     4381 aacgaagccc cctttgagct actggccaag gccaaataaa acctcgtgct cctctgctgt
     4441 ggggcggccc ggcctcgggg ctcagaggcg ctctgtctac agctgggaag actcgcaggc
     4501 cccaagctcc tggccagcgc tgccccggag gtcggcaggc cccttcctcg tcaccttttt
     4561 gttccctccc ccgcctcccg cattcggccg cttcctgact gggattccac agaaaagccg
     4621 agggctgagg agaagtgtga gcgcctccgc ctgtccactg tcccccaaag tcagttcaat
     4681 ccccgacgtc ctccgctagg ctccacccca ccggcccggg cagggcctcc aaggcacctc
     4741 ccacctacgg gtcacccagt cagcccactt ctttctggga caaaggcgtc atcccttaga
     4801 gacagtagga aaatggtatc tcccggaagt tacctcacga cctccaagag cggcttccaa
     4861 ccttgccgga aatgacgaac gagtcaaccg gatcggtgac tgtggagggc gagctgagcc
     4921 ctggccgccg ccacaatggg ccgcgagttt gggaatctga cgcggatgcg gcatgtgatc
     4981 tcctactcct tgagtccgtt cgagcagcgc gcctatccgc acgtcttcac taaaggaatc
     5041 cccaatgttc tgcgccgcat tcgggagtct ttctttcgcg tggtgccgca gtttgtagtg
     5101 ttttatctta tctacacatg ggggactgaa gagttcgaga gatccaagag gaagaatcca
     5161 gctgcctatg aaaatgacaa atgagcaacg catccggatg acggttccct gtctctgaaa
     5221 gacctttctc tggaagagga gtctgcattg tagtgtctca aagacacaat aaacttccta
     5281 tggtctgcac tgttgtgata ttacattttt gtgagtagaa tcctgtgtga ccactaatat
     5341 tcaagttcat gaagcgcccc tcctcagcat gagtcttgaa gttttgcctg gtctcgggtt
     5401 ttggtgaaag agcagggctc cccctttgtt ccattatcta caagaaagta gctgcaggta
     5461 ctcctgcatc taaccatttg tgggtgacat gggctatggc gagtgctggt tcttggccct
     5521 ctgctcccat agcaacaata aagtctacta actatattgt aattagtatg tgtcagggac
     5581 gcttttattt tttatttttt gagatggagt cttgctctct cacccaggct ggagtgcagt
     5641 ggtgtgatct cggctcactg ccatctgtgc ctcctgggtt caaacgattc tcctgtctca
     5701 gcctccgagt agctgggatt acaggtgcag gacaccacac ctggctaatt ttttgtattt
     5761 ttagtagaga caggtttcac catgttggcc aggctggtct ggaactcctg acctcagatg
     5821 atctgtgcgc ctcagcctcc caaagtgctg ggattacagg cgtaagccat ggtgctctgg
     5881 gcctcaaata ctcttcttag ctacacaaat gaactgttaa acctcacaag cacctttaac
     5941 gtatcctcat ttggcagatg aggaaatggg cacagagaga ttaagtaatt tgccagaaat
     6001 tatacagcag tgaatggtgg agtcttgcac taaagtgatg ctcttaaaac tatgctctat
     6061 tgcaggggtg tccaatcttt tagcttccct gggccacaca taaaatacgc taacattaca
     6121 atagctgata agctttaaaa aatggcaaaa aaaaccctca gcgttttaga aagtttacaa
     6181 atttttgttg ggtcggattc aaagccatcc tgggctgctt gctgcccagg actgcggttg
     6241 gacaagcttg ctctactgcc tttcctccta aggcaggcct tagcctgagg ggatatcagc
     6301 tataaaacgg atgaggtgat ctcttagcct ccacagcagt tttctgtttc tgtgccagag
     6361 gccaacaatg ttttcgacca tatgtggttc ataaagcctc aaagatttac tctggccctt
     6421 tatgaataaa gtttacccct gatctaaagc ctttaaatta tctttttggt attaaagata
     6481 ccattttccc ttcgcccacc gagagatggt gggggtaggt gtggttggca gttttatgcc
     6541 tctagttcct tagagaggct tctctctaag cttcgggaca tggatctcac ctgtggtagc
     6601 aggtatgtgg aatatgatga gttgtcatag ctcagtcagt acagtatatg ctatgggatc
     6661 tgagatccgt ttttagaaac tcttctaagg actcttcctg ctagcagcaa ctttgattct
     6721 gggtcagact gtgttttttt tttttttttt tttttgagat gggagtcttg ctctgtcacc
     6781 aggctggagt ttagtggcgc aatctcagct tactgcaacc tccgcttccc gggctcaagt
     6841 gattctcctg cctcagcctc ccaaatagct gggactacag gcgcatacca acacacccag
     6901 ctaatttttg tatttttatt ttttttttta gtagagacgg agtttcacca tgttggccag
     6961 gatggtcttg atctcttgac ctcgtgatcc acctgcctcg acctcccaaa gtgctgggat
     7021 tacaggtgtg agccaccgtg cctggccaga ctgtatcttt attatcctaa ttactgctat
     7081 tgccttttaa gaatcaaaag ttaagatact tcaaaacatg gccagataca gtggctcaca
     7141 cctgtaatcc cagcactttg ggaggctgaa gtggatggat cacttgaggt caggagtttg
     7201 agaccagcct ggccaacatg gtgaaaccct gtctctacta aaaatataaa aattagctga
     7261 gtgtggtggc acacgcctat aatcccagct actcaggagg ctgaggcaga aacgcttgaa
     7321 cctgggaggt ggaggttgca gtgagccaag attgtgccac tgcactccag cctgggtgac
     7381 agagcgagac ttcaaaacaa aacctaaaag ataaactaca ttgtgaggca ggatacgggg
     7441 tttaaaccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     7501 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     7561 gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc
     7621 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     7681 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     7741 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     7801 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     7861 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     7921 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     7981 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg
     8041 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8101 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8221 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact cat
//
