LOCUS       sequence-48926-e        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HIBCH
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1072..1092
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1093..1110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1111..1134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgacagatg cagcagaaga ggtgctattg ggaaaaaaag gttgcacggg agtcataaca
       61 ctaaacagac caaagttcct caatgcactg actcttaata tgattcggca gatttatcca
      121 cagctaaaga agtgggaaca agatcctgaa actttcctga tcattataaa gggagcagga
      181 ggaaaggctt tctgtgccgg gggtgatatc agagtgatct cggaagctga aaaggcaaaa
      241 cagaagatag ctccagtttt cttcagagaa gaatatatgc tgaataatgc tgttggttct
      301 tgccagaaac cttatgttgc acttattcat ggaattacaa tgggtggggg agttggtctc
      361 tcagtccatg ggcaatttcg agtggctaca gaaaagtgtc tttttgctat gccagaaact
      421 gcaataggac tgttccctga tgtgggtgga ggttatttct tgccacgact ccaaggaaaa
      481 cttggttact tccttgcatt aacaggattc agactaaaag gaagagatgt gtacagagca
      541 ggaattgcta cacactttgt agattctgaa aagttggcca tgttagagga agatttgtta
      601 gccttgaaat ctccttcaaa agaaaatatt gcatctgtct tagaaaatta ccatacagag
      661 tctaagattg atcgagacaa gtcttttata cttgaggaac acatggacaa aataaacagt
      721 tgtttttcag ccaatactgt ggaagaaatt attgaaaact tacagcaaga tggttcatct
      781 tttgccctag agcaattgaa ggtaattaat aaaatgtctc caacatctct aaagatcaca
      841 ctaaggcaac tcatggaggg gtcttcaaag accttgcaag aagtactaac tatggagtat
      901 cggctaagtc aagcttgtat gagaggtcat gactttcatg aaggcgttag agctgtttta
      961 attgataaag accagagtcc aaaatggaaa ccagctgatc taaaagaagt tactgaggaa
     1021 gatttgaata atcactttaa gtctttggga agcagtgatt tgaaatttgc agagaacctc
     1081 tacttccaat cgcaccatca tcaccaccat gattacaagg atgacgacga taagtgagga
     1141 tcc
//
