LOCUS       sequence-489264-        1163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-WT
FEATURES             Location/Qualifiers
     source          1..1163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    476..1064
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(529..549)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(947..958)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actagtaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggg
       61 ctgcaggtcg actctagagg atcgaattca tgtcggctac cgctgccacc gtcccgcctg
      121 ccgccccggc cggcgagggt ggcccccctg cacctcctcc aaatcttacc agtaacagga
      181 gactgcagca gacccaggcc caggtggatg aggtggtgga catcatgagg gtgaatgtgg
      241 acaaggtcct ggagcgagac cagaagctat cggaactgga tgatcgcgca gatgccctcc
      301 aggcaggggc ctcccagttt gaaacaagtg cagccaagct caagcgcaaa tactggtgga
      361 aaaacctcaa gatgatgatc atcttgggag tgatttgcgc catcatcctc atcatcatca
      421 tcgtttactt cagcacttaa ggatccgtta acaattcgat atcaagctta tcgataatca
      481 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
      541 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
      601 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
      661 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
      721 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
      781 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
      841 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
      901 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
      961 agcggacctt ccttcccgcn ggcctgctgc cggctctgcg gcctcttccg cgtcttcgct
     1021 tcgccctcag acgagtcgga tctccctttg ggccncntcc ccgcatcgat accntcgann
     1081 tcnagancta naaaancatn nncancannn nagcantacn gcagctaccn nngctgatng
     1141 nngccnggnn tanaagcnnn ana
//
