LOCUS       sequence-48928-e        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACADS
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1156..1176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1177..1194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1195..1218
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcagtctg tggaactgcc cgagacacac cagatgttgc tccagacatg ccgggacttt
       61 gccgagaagg agttgtttcc cattgcagcc caggtggata aggaacatct cttcccagcg
      121 gctcaggtga agaagatggg cgggcttggg cttctggcca tggacgtgcc cgaggagctt
      181 ggcggtgctg gcctcgatta cctggcctac gccatcgcca tggaggagat cagccgcggc
      241 tgcgcctcca ccggagtcat catgagtgtc aacaactctc tctacctggg gcccatcttg
      301 aagtttggct ccaaggagca gaagcaggcg tgggtcacgc ctttcaccag tggtgacaaa
      361 attggctgct ttgccctcag cgaaccaggg aacggcagtg atgcaggagc tgcgtccacc
      421 accgcccggg ccgagggcga ctcatgggtt ctgaatggaa ccaaagcctg gatcaccaat
      481 gcctgggagg cttcggctgc cgtggtcttt gccagcacgg acagagccct gcaaaacaag
      541 agcatcagtg ccttcctggt ccccatgcca acgcctgggc tcacgttggg gaagaaagaa
      601 gacaagctgg gcatccgggg ctcatccacg gccaacctca tctttgagga ctgtcgcatc
      661 cccaaggaca gcatcctggg ggagccaggg atgggcttca agatagccat gcaaaccctg
      721 gacatgggcc gcatcggcat cgcctcccag gccctgggca ttgcccagac cgccctcgat
      781 tgtgctgtga actacgctga gaatcgcatg gccttcgggg cgcccctcac caagctccag
      841 gtcatccagt tcaagttggc agacatggcc ctggccctgg agagtgcccg gctgctgacc
      901 tggcgtgctg ccatgctgaa ggataacaag aagcctttca tcaaggaggc agccatggcc
      961 aagctggccg cctcggaggc cgcgaccgcc atcagccacc aggccatcca gatcctgggc
     1021 ggcatgggct acgtgacaga gatgccggca gagcggcact accgcgacgc ccgcatcact
     1081 gagatctacg agggcaccag cgaaatccag cggctggtga tcgccgggca tctgctcagg
     1141 agctaccgga gcgcagagaa cctctacttc caatcgcacc atcatcacca ccatgattac
     1201 aaggatgacg acgataagtg aggatcc
//
