LOCUS       sequence-489298-       12066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably expresses ER localized LOV-BirA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL95
FEATURES             Location/Qualifiers
     source          1..12066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          20..85
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             215..235
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    660..794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(697..716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     751..770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             962..1595
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1642..1767
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2264..2497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2682..2726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3011..3128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3870..4277
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4992..5048
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5049..5114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6537..6824
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     misc_feature    6891..7479
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6944..6964)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7362..7373)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7482..7498)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7483..7499)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7589..7822
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7847..7865)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7848..7865)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8155..8174)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8388..8407)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8507..8529
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8567..8585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8653..8757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8758..8826,8827..9618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8976..8995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9789..10377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10278..10297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10531..10548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10618..10638)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10623..10952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10803..10938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
       61 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
      121 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
      181 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
      241 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
      301 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
      361 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
      421 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
      481 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
      541 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      781 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
      841 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
      901 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
      961 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     1021 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     1081 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     1141 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     1201 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     1261 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     1321 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     1381 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     1441 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1501 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1561 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1621 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1681 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1741 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1801 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1861 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1921 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1981 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2041 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2101 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     2161 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     2221 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     2281 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     2341 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     2401 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2461 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2521 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2581 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2641 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2701 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2761 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2821 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2881 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2941 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
     3001 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3061 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3121 aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata
     3181 tcgaattcgg gaggtggtcc ctgcagttac gccaatgata acccccgcca gaaaaatctt
     3241 agtagccttc cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg
     3301 gaaacacccg aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg
     3361 cccgcgtctg tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca
     3421 aaggggtact agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca
     3481 ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg
     3541 ctgccaagca gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc
     3601 ccttccctcc ccttggggac caccgggcgc cacgccgcga acggtaagtg ccgcggtcgt
     3661 cggcgcctcc gccctccccc tagggcccca attcccagcg ggcgcggcgc gcggccccgc
     3721 gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggagtcctg
     3781 cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     3841 ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca tggaaaaata
     3901 ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg
     3961 gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa gaacagatgg
     4021 tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga tatggcccaa
     4081 ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg acctgaaatg
     4141 accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc
     4201 tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca gtcctccgac
     4261 agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt gccaccatga
     4321 acatggcgat tatcaaggaa ttcatgaggt tcaaagttca catggaggga tctgtaaacg
     4381 gccacgagtt cgaaatcgag ggagaggggg agggtcgacc atatgaagga acacagaccg
     4441 ctaaattgaa ggttactaaa ggtggaccac ttcctttcgc atgggacatt cttagccccc
     4501 agtttatgta tggttccaag gcgtatgtca aacatcccgc agacattcct gattacctta
     4561 aactgagttt tccagagggg ttcaaatggg agcgcgtcat gaactttgaa gatgggggcg
     4621 ttgttacagt gactcaggac tcttctttgc aagatggaga gtttatatat aaggtgaaac
     4681 ttcgcggaac caacttccca agcgatggcc cagtcatgca gaagaaaact atgggttggg
     4741 aagccagtag cgagcggatg tacccagagg acggcgcact taagggtgag attaagcagc
     4801 ggctgaaact caaagacgga ggccattatg acgcggaagt taaaacgacg tacaaagcta
     4861 aaaagcctgt ccaattgcca ggcgcgtaca atgtcaatat taaattggac attaccagtc
     4921 acaacgaaga ttacacaata gtggagcaat acgaacgcgc agagggacgc cattcaacag
     4981 ggggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     5041 ctggacctga ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg
     5101 atgacgatga caagcttatg aaggataaca ccgtgccact gaaattgatt gccctgttag
     5161 cgaacggtga atttcactct ggcgagcagt tgggtgaaac gctgggaatg agccgggcgg
     5221 ctattaataa acacattcag acactgcgtg actggggcgt tgatgtcttt accgttccgg
     5281 gtaaaggata cagcctgcct gagcctatcc agttacttaa tgctaaacag atattgggtc
     5341 agctggagtt tcgggcaacc acactggaac ggatcgagaa aagcttcgtg attactgatc
     5401 cgagactgcc tgacaaccca atcatttttg tgagcgattc cttcctgcag ctgacagaat
     5461 attctcggga agagatcctg gggcgcaatt gccgttttct gcagggaccc gagacagacc
     5521 gtgccactgt tcggaaaatc agagatgcta ttgacaacca gactgaagtg accgttcagc
     5581 tgatcaatta taccaagagc ggcaagaagt tctggaacgt gttccacctg cagccgatgc
     5641 gcgattataa gggcgacgtc cagtacttca ttggcgtgca gctggatggc accgaacgtc
     5701 ttcatggcgc cgctgagcgt gaggcggtct gcctggtcaa aaagacagcc tttcagattg
     5761 ctgtagccgt gctgccagtg attgactcca cgaatcagta ccttcttgat cgtatcggag
     5821 agcttaaatc gggcgatgct tgcattgcag aataccagca ggctggccgt ggtcgccggg
     5881 gtcggaaatg gttttcgcct tttggcgcaa acttatattt gtcgatgttc tggcgtctgg
     5941 aacaaggccc ggcggcggcg attggtttaa gtctggttat cggtatcgtg atggcggaag
     6001 tattacgcaa gctgggtgca gataaagttc gtgttaaatg gcctaatgac ctctatctgc
     6061 aggatcgcaa gctggcaggc attctggtgg agctgactgg caaaactggc gatgcggcgc
     6121 aaatagtcat tggagccggg atcaacatgg caatgcgccg tgttgaagag agtgtcgtta
     6181 atcaggggtg gatcacgctg caggaagcgg ggatcaatct cgatcgtaat acgttggcgg
     6241 ccatgctaat acgtgaatta cgtgctgcgt tggaactctt cgaacaagaa ggattggcac
     6301 cttatctgtc gcgctgggaa aagctggata attttattaa tcgcccagtg aaacttatca
     6361 ttggtgataa agaaatattt ggcatttcac gcggaataga caaacagggg gctttattac
     6421 ttgagcagga tggaataata aaaccctgga tgggcggtga aatatccctg cgtagtgcag
     6481 aaaaaggagg ttctggtgga ggcgggtctg gaggcggggg tagtaacagc cgcgtgcctg
     6541 gtccgacccc cagtggcact aacgtgggat cctcagggcg ctctcccagc aaagcagtgg
     6601 ccgcccgggc ggcgggatcc actgtccggc agaggaaaaa tgccagctgt gggacaagga
     6661 gtgcaggccg cacaacctcg gcaggcaccg gggggatgtg gcgattctac acagaagatt
     6721 cacctgggct caaagttggc cctgttccag tattggttat gagtcttctg ttcatcgctt
     6781 ctgtatttat gttgcacatt tggggcaagt acactcgttc gtagtaaggc cgcggccgcg
     6841 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     6901 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6961 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7021 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7081 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7141 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7201 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7261 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7321 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7381 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7441 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     7501 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7561 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7621 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
     7861 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     7921 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     7981 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     8041 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     8101 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     8161 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     8221 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     8281 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     8341 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     8401 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8461 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8521 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     8581 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8641 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8701 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8761 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8821 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8881 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8941 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9001 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9061 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9301 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10381 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10441 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10501 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10561 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
    10621 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10681 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10741 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10801 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10861 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10921 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
    10981 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
    11041 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
    11101 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
    11161 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
    11221 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
    11281 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
    11341 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
    11401 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
    11461 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
    11521 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
    11581 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
    11641 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
    11701 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
    11761 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
    11821 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
    11881 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
    11941 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
    12001 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
    12061 acctac
//
