LOCUS       sequence-48930-e        2295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2295)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MUT
FEATURES             Location/Qualifiers
     source          1..2295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2224..2244
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2245..2262
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2263..2286
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtcacctc attacctgag gcaggtaaaa gaatcatcag gctccaggct catacagcaa
       61 cgacttctac accagcaaca gccccttcac ccagaatggg ctgccctggc taaaaagcag
      121 ctgaaaggca aaaacccaga agacctaata tggcacaccc cggaagggat ctctataaaa
      181 cccttgtatt ccaagagaga tactatggac ttacctgaag aacttccagg agtgaagcca
      241 ttcacacgtg gaccatatcc taccatgtat acctttaggc cctggaccat ccgccagtat
      301 gctggtttta gtactgtgga agaaagcaat aagttctata aggacaacat taaggctggt
      361 cagcagggat tatcagttgc ctttgatctg gcgacacatc gtggctatga ttcagacaac
      421 cctcgagttc gtggtgatgt tggaatggct ggagttgcta ttgacactgt ggaagatacc
      481 aaaattcttt ttgatggaat tcctttagaa aaaatgtcag tttccatgac tatgaatgga
      541 gcagttattc cagttcttgc aaattttata gtaactggag aagaacaagg tgtacctaaa
      601 gagaaactta ctggtaccat ccaaaatgat atactaaagg aatttatggt tcgaaataca
      661 tacatttttc ctccagaacc atccatgaaa attattgctg acatatttga atatacagca
      721 aagcacatgc caaaatttaa ttcaatttca attagtggat accatatgca ggaagcaggg
      781 gctgatgcca ttctggagct ggcctatact ttagcagatg gattggagta ctctagaact
      841 ggactccagg ctggcctgac aattgatgaa tttgcaccaa ggttgtcttt cttctgggga
      901 attggaatga atttctatat ggaaatagca aagatgagag ctggtagaag actctgggct
      961 cacttaatag agaaaatgtt tcagcctaaa aactcaaaat ctcttcttct aagagcacac
     1021 tgtcagacat ctggatggtc acttactgag caggatccct acaataatat tgtccgtact
     1081 gcaatagaag caatggcagc agtatttgga gggactcagt ctttgcacac aaattctttt
     1141 gatgaagctt tgggtttgcc aactgtgaaa agtgctcgaa ttgccaggaa cacacaaatc
     1201 atcattcaag aagaatctgg gattcccaaa gtggctgatc cttggggagg ttcttacatg
     1261 atggaatgtc tcacaaatga tgtttatgat gctgctttaa agctcattaa tgaaattgaa
     1321 gaaatgggtg gaatggccaa agctgtagct gagggaatac ctaaacttcg aattgaagaa
     1381 tgtgctgccc gaagacaagc tagaatagat tctggttctg aagtaattgt tggagtaaat
     1441 aagtaccagt tggaaaaaga agacactgta gaagttctgg caattgataa tacttcagtg
     1501 cgaaacaggc agattgaaaa acttaagaag atcaaatcca gcagggatca agctttggct
     1561 gaacgttgtc ttgctgcact aaccgaatgt gctgctagcg gagatggaaa tatcctggct
     1621 cttgcagtgg atgcatctcg ggcaagatgt acagtgggag aaatcacaga tgccctgaaa
     1681 aaggtatttg gtgaacataa agcgaatgat cgaatggtga gtggagcata tcgccaggaa
     1741 tttggagaaa gtaaagagat aacatctgct atcaagaggg ttcataaatt catggaacgt
     1801 gaaggtcgca gacctcgtct tcttgtagca aaaatgggac aagatggcca tgacagagga
     1861 gcaaaagtta ttgctacagg atttgctgat cttggttttg atgtggacat aggccctctt
     1921 ttccagactc ctcgtgaagt ggcccagcag gctgtggatg cggatgtgca tgctgtgggc
     1981 gtaagcaccc tcgctgctgg tcataaaacc ctagttcctg aactcatcaa agaacttaac
     2041 tcccttggac ggccagatat tcttgtcatg tgtggagggg tgataccacc tcaggattat
     2101 gaatttctgt ttgaagttgg tgtttccaat gtatttggtc ctgggactcg aattccaaag
     2161 gctgccgttc aggtgcttga tgatattgag aagtgtttgg aaaagaagca gcaatctgta
     2221 gcagagaacc tctacttcca atcgcaccat catcaccacc atgattacaa ggatgacgac
     2281 gataagtgag gatcc
//
