LOCUS       sequence-48932-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTGR1
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga ctaagacatg gaccctgaag aagcactttg ttggctatcc tactaatagt
      121 gactttgagt tgaagacatc tgagctccca cccttaaaaa atggagaggt cctgcttgaa
      181 gctttgttcc tcaccgtgga tccctacatg agagtggcag ccaaaagatt gaaggaaggt
      241 gatacaatga tggggcagca agtggccaaa gttgtggaaa gtaaaaatgt agccctacca
      301 aaaggaacta ttgtactggc ttctccaggc tggacaacgc actccatttc tgatgggaaa
      361 gatctggaaa agctgctgac agagtggcca gacacaatac cactgtcttt ggctctgggg
      421 acagttggca tgccaggcct gactgcctac tttggcctac ttgaaatctg tggtgtgaag
      481 ggtggagaaa cagtgatggt taatgcagca gctggagctg tgggctcagt cgtggggcag
      541 attgcaaagc tcaagggctg caaagttgtt ggagcagtag ggtctgatga aaaggttgcc
      601 taccttcaaa agcttggatt tgatgtcgtc tttaactaca agacggtaga gtctttggaa
      661 gaaaccttga agaaagcgtc tcctgatggt tatgattgtt attttgataa tgtaggtgga
      721 gagttttcaa acactgttat cggccagatg aagaaatttg gaaggattgc catatgtgga
      781 gccatctcta catataacag aaccggccca cttcccccag gcccaccccc agagattgtt
      841 atctatcagg agcttcgcat ggaagctttt gtcgtctacc gctggcaagg agatgcccgc
      901 caaaaagctc tgaaggactt gctgaaatgg gtcttagagg gtaaaatcca gtacaaggaa
      961 tatatcattg aaggatttga aaacatgcca gccgcattta tgggaatgct gaaaggagat
     1021 aatttgggga agacaatagt gaaagcataa cagtaaaggt ggatacggat ccgaa
//
