LOCUS       sequence-489446-        2871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of wild-type FAM136A downstream of SP6 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Harhai M, Foged MM, Zarges C, Landoni JC, Chollet S, Simonelli M, 
            Recazens E, Lisci M, Laban N, Manley S, Riemer J, Lopez-Escamez JA, 
            Lysakowski A, Jourdain AA
  TITLE     An updated inventory of genes essential for oxidative 
            phosphorylation identifies a mitochondrial origin in familial 
            Meniere's disease.
  JOURNAL   Cell Rep. 2025 Jul 25;44(8):116069. doi: 
            10.1016/j.celrep.2025.116069.
  PUBMED    40714634
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-07-25"; volume: "44(8):116069. doi"; pages: " 
            10.1016/j.celrep.2025.116069"
COMMENT     Sequence Label: pTwist Amp High Copy FAM136A-HA WT
FEATURES             Location/Qualifiers
     source          1..2871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             431..457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(507..526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(508..526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(545..561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(623..639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..658)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(811..1399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(891..910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(1516..1540)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1640..2431,2432..2500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2263..2282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2501..2605)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2719..2735
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2823..2839
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2847..2865
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2847..2864
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attcgagctc ggtaccatgg cagaattgca gcagttacgt gttcaggagg cagtcgagag
       61 catggtgaaa agcttagagc gggagaatat acgtaagatg cagggattga tgttccgatg
      121 ctctgcaagc tgttgcgaag acagtcaggc ctcaatgaaa caggtccatc aatgcattga
      181 gagatgtcat gttccactgg cccaggccca ggctctcgtc acaagtgaat tagagaagtt
      241 ccaggatagg ttggcgaggt gtactatgca ctgcaatgac aaggccaagg acagtatcga
      301 tgctgggagt aaggagcttc aggtgaagca gcaactggac agctgtgtga caaagtgcgt
      361 agatgaccac atgcacttaa ttcctacaat gaccaagaag atgaaggaag ccttgctgtc
      421 catcgggaaa tacccatacg atgttccaga ttacgcttaa tagtgaggat cctctagagt
      481 cgacctgcag gcatgcaagc ttgtctccct atagtgagtc gtattagagc ttggcgtaat
      541 catggtcata gctgtttcct gtgtgaaggc taggtggagg ctcagtgatg ataagtctgc
      601 gatggtggat gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      661 gagggcacaa tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt
      721 tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag
      781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt
     1441 caacaaagcc gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcctc
     1501 gtgataccaa ttcggagcct gcttttttgt acaaacttgt tgataatggc aattcaagga
     1561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agagccacgc tcaccggctc
     1801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2221 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccagat acctgaaaca
     2641 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     2701 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     2761 ataaggaagt gccattccgc ctgacctttg ggtaacgcca gggttttccc agtcacgacg
     2821 ttgtaaaacg acggccagtg aattggattt aggtgacact atagaatacg a
//
