LOCUS       sequence-489465-        2609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector part for BEVA; BEVA2.0 Position 4 I-SceI recognition/cut 
            site, SpR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pQGG001
FEATURES             Location/Qualifiers
     source          1..2609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(599..616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(709..1297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(789..808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2482..2586)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccatgag ctccactctg tgcgtctcat tactgaccat tccaaggtag
      421 ggataacagg gtaatttgga accgtagtcc agatgagacg cacgaagtgg gtacctctta
      481 attaactggc ctcatgggcc ttcacttcac tgcccgcttt ccagtcggga aacctgtcgt
      541 gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct
      601 cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa
      661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1141 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1441 tatatatgag taaacttggt ctgacagtta ttatttaccc accactttgg taatttcgcc
     1501 tttcacgtaa tgcacaaatt cttccagctg atccgcacgg ctggccagac gatcttcttc
     1561 ctggcccaga tacgcctgac gcgcttccag aatcaccggc tgatactgcg ccggcagacg
     1621 ttccatggcc caatccgccg ccacatcttt cggcgcaatt ttgccggtca ccgcgctata
     1681 ccaaatacgg ctcagggtca gcaccacgtt gcgttcatca cccgcccaat ccggcgggct
     1741 gttccacagg gtcagggttt cgttcagcgc ttcaaacagg tcctgttccg gcaccggatc
     1801 aaacagttct tccgctgccg gaccaaccag cgccacgcta tgttcacgcg ctttggtcag
     1861 cagaatggcc agatcgatat caatggtcgc cggttcaaaa atgccggcca gaatatcgtt
     1921 acgctgccat tcgccaaact gcagctcacg tttcgccgga taacgccacg gaataatgtc
     1981 atcatgcacc acgatggtca cttccaccgc acgcagaatt tcgctttcac ccgggctcgc
     2041 gctggtttcc agcagatcgt taatcagcgc acgacgggtg gtttcatcca gacgcacggt
     2101 cacggtcacc agcagatcaa tatcgctatg cggtttcagg ccgccatcca ccgcgctgcc
     2161 atacagatgc acggccagca gggtcggttc cagatgacgt tcaatcacgc ccaccacttc
     2221 agacagctgg gtgctcactt ccgcaatcac cgcttcacgc ataatgttca gtttggtttt
     2281 cggacggctg ccctgctggg tcacatcgtt gctgctccac agcatcaggc aacggcccac
     2341 cgcataacgc gcctgctgtt tgctcgcacg cggcatgctc tgcacgccaa aaaagcaatc
     2401 atagcacgcc ataaacaccg ccaccgcacc attaccgccg ctacgttcgg tcagggtacg
     2461 gctccaatta cggctacgca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2581 tccgcgcaca tttccccgaa aagtgccac
//
