LOCUS       sequence-48948-e        1782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1782)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RECQL
FEATURES             Location/Qualifiers
     source          1..1782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1711..1731
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1732..1749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1750..1773
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtgtttag aggattctga tgccggggca agcaatgaat atgattcttc acctgccgct
       61 tggaataaag aagattttcc atggtctggt aaagttaaag atattctgca aaatgtcttt
      121 aaactggaaa agttcagacc acttcagctt gaaactatta acgtaacaat ggctggaaag
      181 gaggtatttc ttgttatgcc tacaggaggt ggaaagagct tatgttacca gttaccagca
      241 ttatgttcag atggttttac actcgtcatt tgcccattga tctctcttat ggaagaccaa
      301 ttaatggttt taaaacaatt aggaatttca gcaaccatgt taaatgcttc tagttctaag
      361 gagcatgtta aatgggttca tgctgaaatg gtaaataaaa actccgagtt aaagctgatt
      421 tatgtgactc cagagaaaat tgcaaaaagc aaaatgttta tgtcaagact agagaaagcc
      481 tatgaagcaa ggagatttac tcgaattgct gtggatgaag ttcactgctg tagtcagtgg
      541 ggacatgatt tcagacctga ttataaggca cttggtatct taaagcggca gttccctaac
      601 gcatcactaa ttgggctgac tgcaactgca acaaatcacg ttttgacgga tgctcagaaa
      661 attttgtgca ttgaaaagtg ttttactttt acagcttctt ttaataggcc aaatctatat
      721 tatgaggttc ggcagaagcc ctcaaacact gaagatttta ttgaggatat tgtaaagctc
      781 attaatggga gatacaaagg gcaatcagga atcatatatt gtttttctca gaaagactct
      841 gaacaagtta cggttagttt gcagaatctg ggaattcatg caggtgctta ccatgccaat
      901 ttggagccag aagataagac cacagttcat agaaaatggt cagccaatga aattcaggta
      961 gtagtggcaa ctgttgcatt tggtatggga attgataagc cagatgtgag gtttgttatc
     1021 catcattcaa tgagtaaatc catggaaaat tattaccaag agagtggacg tgcaggtcga
     1081 gatgacatga aagcagactg tattttgtac tacggctttg gagatatatt cagaataagt
     1141 tcaatggtgg tgatggaaaa tgtgggacag cagaagcttt atgagatggt atcatactgt
     1201 caaaacataa gcaaatgtcg tcgtgtgttg atggctcaac attttgatga agtatggaac
     1261 tcagaagcat gtaacaaaat gtgcgataac tgctgtaaag acagtgcatt tgaaagaaag
     1321 aacataacag agtactgcag agatctaatc aagatcctga agcaggcaga ggaactgaat
     1381 gaaaaactca ctccattgaa actgattgat tcttggatgg gaaagggtgc agcaaaactg
     1441 agagtagcag gtgttgtggc tcccacactt cctcgtgaag atctggagaa gattattgca
     1501 cactttctaa tacagcagta tcttaaagaa gactacagtt ttacagctta tgctaccatt
     1561 tcgtatttga aaataggacc taaagctaac cttctgaaca atgaggcaca tgctattact
     1621 atgcaagtga caaagtccac gcagaactct ttcagggctg aatcgtctca aacttgtcat
     1681 tctgaacaaa gtgataaaaa gatggaggca gagaacctct acttccaatc gcaccatcat
     1741 caccaccatg attacaagga tgacgacgat aagtgaggat cc
//
