LOCUS       sequence-48950-e        1317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1317)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DMPK
FEATURES             Location/Qualifiers
     source          1..1317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             222..230
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc agcagctggt gttggacccg ggcttcctgg ggctggagcc cctgctcgac
      121 cttctcctgg gcgtccacca ggagctgggc gcctccgaac tggcccagga caagtacgtg
      181 gccgacttct tgcagtgggc ggagcccatc gtggtgaggc ttaaggaggt ccgactgcag
      241 agggacgact tcgagattct gaaggtgatc ggacgcgggg cgttcagcga ggtagcggta
      301 gtgaagatga agcagacggg ccaggtgtat gccatgaaga tcatgaacaa gtgggacatg
      361 ctgaagaggg gcgaggtgtc gtgcttccgt gaggagaggg acgtgttggt gaatggggac
      421 cggcggtgga tcacgcagct gcacttcgcc ttccaggatg agaactacct gtacctggtc
      481 atggagtatt acgtgggcgg ggacctgctg acactgctga gcaagtttgg ggagcggatt
      541 ccggccgaga tggcgcgctt ctacctggcg gagattgtca tggccataga ctcggtgcac
      601 cggcttggct acgtgcacag ggacatcaaa cccgacaaca tcctgctgga ccgctgtggc
      661 cacatccgcc tggccgactt cggctcttgc ctcaagctgc gggcagatgg aacggtgcgg
      721 tcgctggtgg ctgtgggcac cccagactac ctgtcccccg agatcctgca ggctgtgggc
      781 ggtgggcctg ggacaggcag ctacgggccc gagtgtgact ggtgggcgct gggtgtattc
      841 gcctatgaaa tgttctatgg gcagacgccc ttctacgcgg attccacggc ggagacctat
      901 ggcaagatcg tccactacaa ggagcacctc tctctgccgc tggtggacga aggggtccct
      961 gaggaggctc gagacttcat tcagcggttg ctgtgtcccc cggagacacg gctgggccgg
     1021 ggtggagcag gcgacttccg gacacatccc ttcttctttg gcctcgactg ggatggtctc
     1081 cgggacagcg tgcccccctt tacaccggat ttcgaaggtg ccaccgacac atgcaacttc
     1141 gacttggtgg aggacgggct cactgccatg gtgagcgggg gcggggagac actgtcggac
     1201 attcgggaag gtgcgccgct aggggtccac ctgccttttg tgggctactc ctactcctgc
     1261 atggccctca gggacagtga ggtcccaggc cccacaccct gacagtaaag gtggata
//
