LOCUS       sequence-48954-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPSB4
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gtgcagagcc cgggcggccg gcgcggctgg accagctgtt ggacatgcca
      121 gcggcggggc tggctgtgca gctgcggcac gcgtggaacc ccgaggaccg ctcgctcaac
      181 gtcttcgtca aggacgacga ccggctcacc ttccaccggc accccgtggc ccagagcacc
      241 gacggcatcc gcggcaaggt gggccacgcc cgcggcctgc acgcctggca gatcaactgg
      301 ccggctcggc agcgcggcac ccacgctgta gttggtgtgg ccacggcccg tgctcccctg
      361 cactccgtgg gctacacggc gctggtaggc agtgacgccg agtcgtgggg ctgggacctg
      421 ggccgcagcc gcctctacca cgacggcaag aaccagcccg gcgtggccta cccggccttt
      481 ctggggcccg acgaggcctt tgcgctgccc gactcgctgc tcgtggtgct ggacatggat
      541 gagggcacac tcagcttcat cgtggatggc cagtacctgg gcgtggcctt ccgaggtctc
      601 aagggcaaga agctgtaccc ggtggtgagt gccgtgtggg gccactgtga agtcaccatg
      661 cgctacatca acggccttga ccccgagtga cagtaaaggt ggata
//
