LOCUS       sequence-489544-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targets high levels of recombinant protein to vacuole. Empty vector.
            Clone via SapI restriction site. mCherry dropout cassette will 
            enable Red/White screening in E.coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Chartrand-Pleau C, Gallipeau-Burns E, Jain A, Boddy V, Fakhouri P, 
            Rehmani S, Thomas T, Drapeau M, VanderBurgt JT, Boudigou A, Damry 
            AM, MacLean AM
  TITLE     pTARGEX Vectors: A Versatile Toolbox for Plant-Based Protein 
            Expression
  JOURNAL   Front. Synth. Biol. 3:1619871
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front. 
            Synth. Biol. 3:1619871"
COMMENT     Sequence Label: pTARGEX-VAC
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        110..155
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    434..455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(470..475,476..493,494..500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             564..586
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      1374..1401
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1660..1912
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2060..2405
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3878..4054
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4620..4799
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4652..4835
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4856..4858,4859..4861,4862..5572)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      5961..6213
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6304..6328
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6696..7327
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7417..8565)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8864..9658)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(10248..10265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10499..10518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10950..10974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11113..11132
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11257..11279
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11271..11288
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11272..11288
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
       61 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      121 cctctatata aggaagttca tttcatttgg agaggtttaa gagacgcaac cacaacgctc
      181 taacgcaatc aatctacatt atattaaacg tctctaaaaa gaaaccatgg gattttttct
      241 cttttcacaa atgccctcat tttttcttgt gtcgacactt ctcttattcc taataatatc
      301 tcactcttct catgccggct gaagagcatc aatcaatacg caaaccgcct ctccccgcgc
      361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaagaccac
      541 aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata catatggttt
      601 ccaagggaga ggaagacaat atggcgatta tcaaagagtt catgcgcttc aaggtccaca
      661 tggaaggaag tgtgaacggg catgagttcg aaatagaagg cgaaggtgag ggtcgcccgt
      721 acgaaggtac gcaaaccgca aagctgaagg ttactaaggg tgggcctctg ccgttcgcat
      781 gggacatctt atctccgcaa ttcatgtacg gtagtaaggc ttacgtaaag caccccgccg
      841 atattcccga ctacttgaag ttaagtttcc cagaggggtt caagtgggag agagtaatga
      901 atttcgagga cggcggggta gtcaccgtaa cccaagactc atctcttcaa gacggtgagt
      961 ttatatacaa ggtgaagctg cgcgggacga acttcccatc tgacggacca gtgatgcaga
     1021 agaagactat gggatgggaa gccagtagcg aaagaatgta cccagaagac ggggccctta
     1081 aaggagaaat taagcagcgg ctcaagttga aggatggcgg gcactatgat gcagaggtca
     1141 aaaccaccta caaggcaaag aaaccagtac aattgcccgg tgcgtacaac gtaaacatta
     1201 aattagacat cacttctcat aatgaggact acacaatagt ggagcaatac gagcgagcag
     1261 aggggagaca ctctacgggt ggtatggacg agttgtataa ataagaattc gaagcttgat
     1321 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccatcac actggctcac
     1381 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     1441 aatccggctt ttttattatt tgagttgtat aagctcttca aacggtttgc ttgttgatac
     1501 tatgtgatag ataaactgtt attcggtgtg catttctatg tttggtgagc ggttttctgt
     1561 gctcagagtg tgtttatttt atgtaattta atttctttgt gagctcctgt ttagcaggtc
     1621 gtcccttcag caaggacaca aaaagatttt aattttattg atcgttcaaa catttggcaa
     1681 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     1741 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     1801 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     1861 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcggcgcgcc
     1921 agcttggcgt aatcatggtc atagctgttg cgattaataa ttccacgcgg atcgcattga
     1981 gaacccggaa tatcgcgaaa caggtacccc cctactccaa aaatgtcaaa gatacagtct
     2041 cagaagacca aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg
     2101 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct
     2161 cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca
     2221 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     2281 ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac
     2341 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     2401 ggacagccca agcttcgact ctagaggatc cccttaaatc gatatgagca ccgccaagat
     2461 gagcgctggt gagttctcta agacctacgg caccaaggat agccggtctg tgaatgattg
     2521 ctacgccatc tacagcggca acggcatcaa cttcatcaac ctgttcatgc acaccaacac
     2581 cggcatcaag agcgccttct ctattaacga ccttggccgg aacgaggaca ttaagcttca
     2641 tgaggccgag atcatcaaca gcttgcaccc gtactcattc ttcgacaagt tcggcctgga
     2701 tatcatcctg tgcaaccacg tgatggacat catcgtgact aagcctggcc ttaagaacac
     2761 cggatgcaag ttccagatgc acaaccagat cttcaacccg aatgaggata ttctggctaa
     2821 gacccctggc atcatcagcg aagagaactt ctacgacctg agcaagatca agcctaaggg
     2881 catgacacct ttcgactggt acatcaacga gtgcaagaag tacgacttct acatcagcga
     2941 caacggcgac atctctctgg attacggttt ccctgtgatg ggcaagacca cctcttattg
     3001 gagagagaac atcccgcgag agaagatcat ctccgttaag cagaagtgcc tgccgaacac
     3061 ctctgctctt accaacagga ttctgagcct ttctaccgtg agggctatcc agattgcttc
     3121 tgagctggct tctgaaaaga ccgtggttct tgcttctagg cagaggctgg atctggacat
     3181 caagtctcag taccggatct cattccctgg tgttcaagat gagggcgctt tcactaggac
     3241 tttctgcatc cctatggaca agaccgctag gatcgtttgc ttctacgcta agacctccgt
     3301 ggacgtgtca aatgagagga ccaccttgac catcaagatc gtcaacaaga ccgtcgagag
     3361 caactacatg ggtcctgtgc ctaaggatca catgtactgc gataagagca tcggcgctag
     3421 gattggtctt gtggatattg tgaggggcga cccgaactac aatcagatga ttgctcggga
     3481 aatgatcagc gtccacacca actttgctct gaggctttct gaggctctga agaagccggt
     3541 catcgttttc aagatgtacg agaaagagct gaacttcgag acttacgacc tgtccggtcg
     3601 gtctttgtct taccagaagg acagcagcgg caatatctac ttcctgtcta ggacccttga
     3661 gatcctgccg aagtctcttt ctaccctgac ctacctgaag aacatctctc ctgcctgctg
     3721 gaaagaatcc atcagcatgc agcatttcta catcggggag caagaagagg gaccttctgg
     3781 ttctaatctg gccgagtctg aagagaaggt gcagactgct taggaattcg gtacgctgaa
     3841 atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag
     3901 tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     3961 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     4021 cctaaaacca aaatccagta ctaaaatcca gatctcctaa agtccctata gatctttgtc
     4081 gtgaatataa accagacacc ggacgactaa acctggagcc cagacgccgt tcgaagctag
     4141 aagtaccgct taggcaggag gccgttaggg aaaagatgct aaggcagggt tggttacgtt
     4201 gactcccccg taggtttggt ttaaatatga tgaagtggac ggaaggaagg aggaagacaa
     4261 ggaaggataa ggttgcaggc cctgtgcaag gtaagaagat ggaaatttga tagaggtacg
     4321 ctactatact tatactatac gctaagggaa tgcttgtatt tataccctat accccctaat
     4381 aaccccttat caatttaaga aataatccgc ataagccccc gcttaaaaat tggtatcaga
     4441 gccatgaata ggtctatgac caaaactcaa gaggataaaa cctcaccaaa atacgaaaga
     4501 gttcttaact ctaaagataa aagatggcgc gtggccggcc atgagcggag aattaaggga
     4561 gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag
     4621 aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac
     4681 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa
     4741 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
     4801 agtctcatat tcactctcaa tccaaataat ctgcaccgga tctggatcgt ttcgcatggt
     4861 ttcaaaggga gaagaacttt tcaccggggt tgtccctata ctggtggaac tggatggaga
     4921 cgtcaacggg cataagtttt ctgtctctgg cgaaggggag ggtgacgcaa cgtacgggaa
     4981 gctgacactg aagttcatct gtactaccgg caagctcccg gtaccctggc ctacgctggt
     5041 aacaacgttg acttacgggg tgcagtgttt ctctcgatat cctgaccata tgaaacaaca
     5101 cgatttcttc aagagtgcaa tgcctgaggg gtatgttcaa gagcgaacta tattcttcaa
     5161 agatgatggg aactacaaga cacgtgcaga agttaagttt gaaggcgata cgctggtaaa
     5221 ccgaattgaa ttgaaaggga tagattttaa ggaagacggg aatatcctgg gacacaaact
     5281 tgaatataac tataactccc acaacgttta cattatggcc gataaacaga agaatggcat
     5341 caaggtaaat ttcaaaattc gtcacaacat agaagatggg tccgttcagc tcgccgacca
     5401 ttatcaacaa aacactccaa taggggatgg ccccgtactg ttgcccgata accactattt
     5461 gagcacgcaa tcagcacttt ctaaggaccc aaatgaaaaa cgtgatcata tggtactctt
     5521 agaattcgtg actgctgctg gtatcacgct tggtatggat gaattgtaca agtgagcggg
     5581 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5641 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5701 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccacgggat ctctgcgaaa
     5761 caggcggtcg aaggtgccga tatcattacg acagcaacgg ccgacaagca caacgccacg
     5821 atcctgagcg acaatatgat cgcggcgtcc acatcaacgg cgtcggcggc gactgcccag
     5881 gcaagaccga gatgcaccgc gatatcttgc tgcgttcgga tattttcgtg gagttcccgc
     5941 cacagacccg gatgatcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     6001 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     6061 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     6121 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     6181 tatcgcgcgc ggtgtcatct atgttactag atcgggggcc ggccctcact ggtgaaaaga
     6241 aaaaccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     6301 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     6361 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     6421 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     6481 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     6541 tgacagagcg ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca
     6601 gccttcttat tcatttctcg cttaaccgtg acagagtaga caggctgtct cgcggccgag
     6661 gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg
     6721 gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac
     6781 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa
     6841 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa
     6901 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca
     6961 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt
     7021 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg
     7081 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg
     7141 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc
     7201 cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc
     7261 tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt
     7321 gagcgtcgga aaggcgctcg gtcttgcctt gctcgtcggt gatgtacact agtcgctggc
     7381 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     7441 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     7501 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     7561 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     7621 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     7681 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     7741 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     7801 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     7861 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     7921 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     7981 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     8041 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     8101 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     8161 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     8221 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     8281 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga
     8341 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     8401 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     8461 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     8521 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     8581 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     8641 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     8701 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     8761 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     8821 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca
     8881 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     8941 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     9001 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     9061 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     9121 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     9181 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     9241 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     9301 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     9361 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     9421 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     9481 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     9541 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     9601 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     9661 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     9721 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     9781 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     9841 acaattatgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     9901 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     9961 taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg
    10021 gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat
    10081 ggccatgaat ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac
    10141 gattttacgt cacttaaaaa actcaggccg cagtcggtaa ctatgcggtg tgaaataccg
    10201 cacagatgcg taaggagaaa ataccgcatc aggcgctatt ccgcttcctc gctcactgac
    10261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    10381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10741 gtaagacacg acttatcgcc actggcagca ggtaacctcg cgcatacagc cgggcagtga
    10801 cgtcatcgtc tgcgcggaaa tggacgggcc cccggcgcca gatctgggga accctgtggt
    10861 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
    10921 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
    10981 gatagtttgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    11041 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    11101 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgcc attcaggctg cgcaactgtt
    11161 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    11221 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    11281 cggccagtga attgttagag ggagctctga tcactgagca tcgttgaaga tgcctctgcc
    11341 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    11401 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
    11461 gcacaatccc actatccttc ttaattaagg aaacctcctc ggattccatt gcccagctat
    11521 ctgtcacttt attgagaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
    11581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
    11641 cccaccca
//
