LOCUS       sequence-489545-       11636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targets high levels of recombinant protein for retention in ER. 
            Empty vector. Clone via SapI restriction site. mCherry dropout 
            cassette will enable Red/White screening in E.coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Chartrand-Pleau C, Gallipeau-Burns E, Jain A, Boddy V, Fakhouri P, 
            Rehmani S, Thomas T, Drapeau M, VanderBurgt JT, Boudigou A, Damry 
            AM, MacLean AM
  TITLE     pTARGEX Vectors: A Versatile Toolbox for Plant-Based Protein 
            Expression
  JOURNAL   Front. Synth. Biol. 3:1619871
REFERENCE   2  (bases 1 to 11636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front. 
            Synth. Biol. 3:1619871"
COMMENT     Sequence Label: pTARGEX-ER
FEATURES             Location/Qualifiers
     source          1..11636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        110..155
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    434..455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(470..475,476..493,494..500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             564..586
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      1374..1401
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1648..1900
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2048..2393
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3866..4042
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4608..4787
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4640..4823
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4844..4846,4847..4849,4850..5560)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      5949..6201
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6292..6316
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6684..7315
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(7405..8553)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8852..9646)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(10236..10253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10487..10506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10938..10962
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11101..11120
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11245..11267
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11259..11276
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11260..11276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
       61 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      121 cctctatata aggaagttca tttcatttgg agaggtttaa gagacgcaac cacaacgctc
      181 taacgcaatc aatctacatt atattaaacg tctctaaaaa gaaaccatgg gattttttct
      241 cttttcacaa atgccctcat tttttcttgt gtcgacactt ctcttattcc taataatatc
      301 tcactcttct catgccggct gaagagcatc aatcaatacg caaaccgcct ctccccgcgc
      361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaagaccac
      541 aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata catatggttt
      601 ccaagggaga ggaagacaat atggcgatta tcaaagagtt catgcgcttc aaggtccaca
      661 tggaaggaag tgtgaacggg catgagttcg aaatagaagg cgaaggtgag ggtcgcccgt
      721 acgaaggtac gcaaaccgca aagctgaagg ttactaaggg tgggcctctg ccgttcgcat
      781 gggacatctt atctccgcaa ttcatgtacg gtagtaaggc ttacgtaaag caccccgccg
      841 atattcccga ctacttgaag ttaagtttcc cagaggggtt caagtgggag agagtaatga
      901 atttcgagga cggcggggta gtcaccgtaa cccaagactc atctcttcaa gacggtgagt
      961 ttatatacaa ggtgaagctg cgcgggacga acttcccatc tgacggacca gtgatgcaga
     1021 agaagactat gggatgggaa gccagtagcg aaagaatgta cccagaagac ggggccctta
     1081 aaggagaaat taagcagcgg ctcaagttga aggatggcgg gcactatgat gcagaggtca
     1141 aaaccaccta caaggcaaag aaaccagtac aattgcccgg tgcgtacaac gtaaacatta
     1201 aattagacat cacttctcat aatgaggact acacaatagt ggagcaatac gagcgagcag
     1261 aggggagaca ctctacgggt ggtatggacg agttgtataa ataagaattc gaagcttgat
     1321 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccatcac actggctcac
     1381 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     1441 aatccggctt ttttattatt tgagttgtat gctcttcaaa agatgaactt tgatagaaat
     1501 aaactgttat tcggtgtgca tttctatgtt tggtgagcgg ttttctgtgc tcagagtgtg
     1561 tttattttat gtaatttaat ttctttgtga gctcctgttt agcaggtcgt cccttcagca
     1621 aggacacaaa aagattttaa ttttattgat cgttcaaaca tttggcaata aagtttctta
     1681 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     1741 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     1801 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     1861 gataaattat cgcgcgcggt gtcatctatg ttactagatc ggcgcgccag cttggcgtaa
     1921 tcatggtcat agctgttgcg attaataatt ccacgcggat cgcattgaga acccggaata
     1981 tcgcgaaaca ggtacccccc tactccaaaa atgtcaaaga tacagtctca gaagaccaaa
     2041 gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga ttccattgcc
     2101 cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc
     2161 atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt ggtcccaaag
     2221 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     2281 agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa tcccactatc
     2341 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acagcccaag
     2401 cttcgactct agaggatccc cttaaatcga tatgagcacc gccaagatga gcgctggtga
     2461 gttctctaag acctacggca ccaaggatag ccggtctgtg aatgattgct acgccatcta
     2521 cagcggcaac ggcatcaact tcatcaacct gttcatgcac accaacaccg gcatcaagag
     2581 cgccttctct attaacgacc ttggccggaa cgaggacatt aagcttcatg aggccgagat
     2641 catcaacagc ttgcacccgt actcattctt cgacaagttc ggcctggata tcatcctgtg
     2701 caaccacgtg atggacatca tcgtgactaa gcctggcctt aagaacaccg gatgcaagtt
     2761 ccagatgcac aaccagatct tcaacccgaa tgaggatatt ctggctaaga cccctggcat
     2821 catcagcgaa gagaacttct acgacctgag caagatcaag cctaagggca tgacaccttt
     2881 cgactggtac atcaacgagt gcaagaagta cgacttctac atcagcgaca acggcgacat
     2941 ctctctggat tacggtttcc ctgtgatggg caagaccacc tcttattgga gagagaacat
     3001 cccgcgagag aagatcatct ccgttaagca gaagtgcctg ccgaacacct ctgctcttac
     3061 caacaggatt ctgagccttt ctaccgtgag ggctatccag attgcttctg agctggcttc
     3121 tgaaaagacc gtggttcttg cttctaggca gaggctggat ctggacatca agtctcagta
     3181 ccggatctca ttccctggtg ttcaagatga gggcgctttc actaggactt tctgcatccc
     3241 tatggacaag accgctagga tcgtttgctt ctacgctaag acctccgtgg acgtgtcaaa
     3301 tgagaggacc accttgacca tcaagatcgt caacaagacc gtcgagagca actacatggg
     3361 tcctgtgcct aaggatcaca tgtactgcga taagagcatc ggcgctagga ttggtcttgt
     3421 ggatattgtg aggggcgacc cgaactacaa tcagatgatt gctcgggaaa tgatcagcgt
     3481 ccacaccaac tttgctctga ggctttctga ggctctgaag aagccggtca tcgttttcaa
     3541 gatgtacgag aaagagctga acttcgagac ttacgacctg tccggtcggt ctttgtctta
     3601 ccagaaggac agcagcggca atatctactt cctgtctagg acccttgaga tcctgccgaa
     3661 gtctctttct accctgacct acctgaagaa catctctcct gcctgctgga aagaatccat
     3721 cagcatgcag catttctaca tcggggagca agaagaggga ccttctggtt ctaatctggc
     3781 cgagtctgaa gagaaggtgc agactgctta ggaattcggt acgctgaaat caccagtctc
     3841 tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat
     3901 aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     3961 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     4021 atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac
     4081 cagacaccgg acgactaaac ctggagccca gacgccgttc gaagctagaa gtaccgctta
     4141 ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta
     4201 ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg
     4261 ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta
     4321 tactatacgc taagggaatg cttgtattta taccctatac cccctaataa ccccttatca
     4381 atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg
     4441 tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt tcttaactct
     4501 aaagataaaa gatggcgcgt ggccggccat gagcggagaa ttaagggagt cacgttatga
     4561 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     4621 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     4681 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     4741 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     4801 actctcaatc caaataatct gcaccggatc tggatcgttt cgcatggttt caaagggaga
     4861 agaacttttc accggggttg tccctatact ggtggaactg gatggagacg tcaacgggca
     4921 taagttttct gtctctggcg aaggggaggg tgacgcaacg tacgggaagc tgacactgaa
     4981 gttcatctgt actaccggca agctcccggt accctggcct acgctggtaa caacgttgac
     5041 ttacggggtg cagtgtttct ctcgatatcc tgaccatatg aaacaacacg atttcttcaa
     5101 gagtgcaatg cctgaggggt atgttcaaga gcgaactata ttcttcaaag atgatgggaa
     5161 ctacaagaca cgtgcagaag ttaagtttga aggcgatacg ctggtaaacc gaattgaatt
     5221 gaaagggata gattttaagg aagacgggaa tatcctggga cacaaacttg aatataacta
     5281 taactcccac aacgtttaca ttatggccga taaacagaag aatggcatca aggtaaattt
     5341 caaaattcgt cacaacatag aagatgggtc cgttcagctc gccgaccatt atcaacaaaa
     5401 cactccaata ggggatggcc ccgtactgtt gcccgataac cactatttga gcacgcaatc
     5461 agcactttct aaggacccaa atgaaaaacg tgatcatatg gtactcttag aattcgtgac
     5521 tgctgctggt atcacgcttg gtatggatga attgtacaag tgagcgggac tctggggttc
     5581 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5641 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5701 cgcggggatc tcatgctgga gttcttcgcc cacgggatct ctgcgaaaca ggcggtcgaa
     5761 ggtgccgata tcattacgac agcaacggcc gacaagcaca acgccacgat cctgagcgac
     5821 aatatgatcg cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     5881 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     5941 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     6001 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     6061 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     6121 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     6181 tgtcatctat gttactagat cgggggccgg ccctcactgg tgaaaagaaa aaccacccca
     6241 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     6301 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     6361 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     6421 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     6481 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     6541 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
     6601 atttctcgct taaccgtgac agagtagaca ggctgtctcg cggccgaggg gcgcagcccc
     6661 tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc
     6721 cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa
     6781 tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc
     6841 cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc
     6901 tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt
     6961 cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg
     7021 tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt
     7081 cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc
     7141 tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc
     7201 acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc
     7261 agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcggaaa
     7321 ggcgctcggt cttgccttgc tcgtcggtga tgtacactag tcgctggctg ctgaaccccc
     7381 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
     7441 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
     7501 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
     7561 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
     7621 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
     7681 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
     7741 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
     7801 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
     7861 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
     7921 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
     7981 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
     8041 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
     8101 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
     8161 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt
     8221 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
     8281 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcaaga cgacgcgaac gctccacggc
     8341 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
     8401 cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
     8461 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
     8521 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac
     8581 tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag
     8641 ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta
     8701 cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc
     8761 gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt
     8821 gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt aaaatataat
     8881 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     8941 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     9001 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     9061 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
     9121 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
     9181 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
     9241 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
     9301 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
     9361 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
     9421 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
     9481 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
     9541 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
     9601 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
     9661 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
     9721 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
     9781 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac aattatgggt
     9841 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     9901 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     9961 caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt
    10021 cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg
    10081 cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca
    10141 cttaaaaaac tcaggccgca gtcggtaact atgcggtgtg aaataccgca cagatgcgta
    10201 aggagaaaat accgcatcag gcgctattcc gcttcctcgc tcactgactc gctgcgctcg
    10261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10741 ttatcgccac tggcagcagg taacctcgcg catacagccg ggcagtgacg tcatcgtctg
    10801 cgcggaaatg gacgggcccc cggcgccaga tctggggaac cctgtggttg gcatgcacat
    10861 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
    10921 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttgtga
    10981 accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    11041 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    11101 agggaagaaa gcgaaaggag cgggcgccat tcaggctgcg caactgttgg gaagggcgat
    11161 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11221 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
    11281 tgttagaggg agctctgatc actgagcatc gttgaagatg cctctgccga cagtggtccc
    11341 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
    11401 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
    11461 tatccttctt aattaaggaa acctcctcgg attccattgc ccagctatct gtcactttat
    11521 tgagaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
    11581 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc caccca
//
