LOCUS       sequence-489548-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eas1 recombitron 
            with extended a1 a2 regions in the ncRNA targeting lacZ locus in 
            Klebsiella pneumoniae ATCC 10031 expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-opEas1
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1447..1541
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1662..1681)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1673..2488
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2655..2763
                     /note="incP origin of transfer"
     CDS             complement(2774..3625)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3612..4451)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4962..5933)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6040..6434
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6472..6558)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6583..7548)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        8448..8528
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             join(10078..10085,1)
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttggaatga accaaatcgt gaagttcact gacgactctg gcctggcggt tcaagttact
       61 ccagacgatg ttcgccgtta tatctgtgag aacgctactg aaaaagaggt gggcctcttt
      121 ctgcaactct gtcagactca acgtctgaat ccgtttgtga aagacgctta cctggtgaaa
      181 tacggcggtg ctccagcttc tatgattact tcctatcaag tttttaaccg tcgcgcgtgt
      241 cgtgatgcta actatgatgg tatcaaatct ggtgtggttg ttctgcgtga cggtgatgtt
      301 gtgcataaac gtggtgctgc gtgctacaaa aaggcgggtg aggagctcat cggtggttgg
      361 gcggaagttc gctttaagga tggccgcgag actgcgtatg ctgaggtggc gctcgacgac
      421 tattccaccg gcaaatctaa ttgggcgaaa atgccgggtg ttatgatcga aaaatgcgcg
      481 aaggctgctg cttggcgcct cgcgttcccg gacacttttc agggcatgta cgctgcggag
      541 gaaatggatc aagcgcaaca gccagaacag gtgcgcgctc aggcggagca accagtggat
      601 ctccagccaa tccgcgaact cttcaagcca tattgcgaac acttcggcat cactccggct
      661 gagggtatga ctgctgtttg tggtgcggtg ggcgctgaag gcatgcactc tatgaccgag
      721 cagcaagctc gccgtgctcg cgcttggatg gaggaagaaa tggctgcgcc agctgtggaa
      781 gcggagtatg aggttgttga cgagggcgag gtgttttaag gatccaggag aatacacatg
      841 gccagcagag gcgtaaacaa ggttattctc gttggtaatc tgggtcagga cccggaagta
      901 cgctacatgc caaatggtgg cgcagttgcc aacattacgc tggctacttc cgaatcctgg
      961 cgtgataaag cgaccggcga gatgaaagag cagactgaat ggcaccgcgt tgtgctgttc
     1021 ggcaaactgg cagaagtggc cagcgaatat ctgcgtaaag gttctcaggt ttatatcgaa
     1081 ggtcagctgc gtacccgtaa atggaccgat caatccggtc aggatcgcta caccacagaa
     1141 gtcgtggtga acgttggcgg caccatgcag atgctgggtg gtcgtcaggg tggtggcgct
     1201 ccggcaggtg gtaatatcgg tggtggtcag ccgcagggcg gttggggtca gcctcagcag
     1261 ccgcagggtg gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca gtccgctccg
     1321 gcagcgccgt ctaacgagcc gccgatggac tttgatgatg acattccgtt ctgatctaga
     1381 gtcgacctgc aggcatgcaa gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta
     1441 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1501 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     1561 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1621 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     1681 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
     1741 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
     1801 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
     1861 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
     1921 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
     1981 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
     2041 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
     2101 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
     2161 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
     2221 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
     2281 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
     2341 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
     2401 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     2461 tcatctgatg ctggatgaat ttttctaata attaattgga ccgcggtccg cgcgttgtcc
     2521 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     2581 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     2641 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     2701 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     2761 ccgtaggccg gcctcagcct gccgccttgg gccgggtgat gtcgtacttg cccgccgcga
     2821 actcggttac cgtccagccc agcgcgacca gctccggcaa cgcctcgcgc acccgctggc
     2881 ggcgcttgcg catggtcgaa ccactggcct ctgacggcca gacatagccg cacaaggtat
     2941 ctatggaagc cttgccggtt ttgccggggt cgatccagcc acacagccgc tggtgcagca
     3001 ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat gctgatgcgc acatgctggc
     3061 cgccacccat gacggcctgc gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg
     3121 cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg ccgcttgcgg ccattctggg
     3181 cgatgatgga taccttccaa aggcgctcga tgcagtcctg tatgtgcttg agcgccccac
     3241 cactatcgac ctctgccccg atttcctttg ccagcgcccg atagctacct ttgaccacca
     3301 tggcatcagc ggtgacggcc tcccacttgg gttccaggaa cagccggagc tgccgtccgc
     3361 cttcggtctt gggttccggg ccaagcacta ggccattagg cccagccatg gccaccagcc
     3421 cttgcaggat gcgcagatca tcagcgccca gcggctccgg gccgctgaac tcgatccgct
     3481 tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga
     3541 gggcacggaa caggccgggg gccagacagt gcgccgggtc gtgccggacg tggctgaggc
     3601 tgtgcttgtt cttaggcttc accacggggc acccccttgc tcttgcgctg cctctccagc
     3661 acggcgggct tgagcacccc gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg
     3721 ccatagttgg ccttgctcac accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca
     3781 ccccattcct cggcctcggc gctggtcatg ctcgacaggt aggactgcca gcggatgtta
     3841 tcgaccagta ccgagctgcc ccggctggcc tgctgctggt cgcctgcgcc catcatggcc
     3901 gcgcccttgc tggcatggtg caggaacacg atagagcacc cggtatcggc ggcgatggcc
     3961 tccatgcgac cgatgacctg ggccatgggg ccgctggcgt tttcttcctc gatgtggaac
     4021 cggcgcagcg tgtccagcac catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg
     4081 aaccactccg gggccatgat gttgggcagg ctgccgatca gcggctggat cagcaggccg
     4141 tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc caagggcgtg caggcggtga
     4201 tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca ccgggccggt gggcagttcg
     4261 cccacctcca gcagatccgg cccgcctgca atctgtgcgg ccagttgcag ggccagcatg
     4321 gatttaccgg caccaccggg cgacaccagc gccccgaccg taccggccac catgttgggc
     4381 aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct ccagaatatt gataggctta
     4441 tgggtagcca ttgattgcct cctttgcagg cagttggtgg ttaggcgctg gcggggtcac
     4501 tacccccgcc ctgcgccgct ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt
     4561 cgtcggtcag ccagaacttg cgctgacgca tccctttggc cttcatgcgc tcggcatatc
     4621 gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc ggtctgcttg tccttttggt
     4681 ctttcatatc agtcaccgag aaacttgccg gggccgaaag gcttgtcttc gcggaacaag
     4741 gacaaggtgc agccgtcaag gttaaggctg gccatatcag cgactgaaaa gcggccagcc
     4801 tcggccttgt ttgacgtata accaaagcca ccgggcaacc aatagccctt gtcacttttg
     4861 atcaggtaga ccgaccctga agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt
     4921 gagtactcat agtataacag gcgtgagtac caacgcaagc actacatgct gaaatctggc
     4981 ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc gtgccagctc ggcccgcgca
     5041 agctggacgc tgggcagacc catgaccttg ctgacggtgc gctcgatgta atccgcttcg
     5101 tggccgggct tgcgctctgc cagcgctggg ctggcctcgg ccatggcctt gccgatttcc
     5161 tcggcactgc ggccccggct ggccagcttc tgcgcggcga taaagtcgca cttgctgagg
     5221 tcatcaccga agcgcttgac cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg
     5281 cgccggtggc ggctaagctg ccgctcgggc agttcgaggc tggccagcct gcgggccttc
     5341 tcctgctgcc gctgggcctg ctcgatctgc tggccagcct gctgcaccag cgccgggcca
     5401 gcggtggcgg tcttgccctt ggattcacgc agcagcaccc acggctgata accggcgcgg
     5461 gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc ggccatagtg gcggctgtcg
     5521 gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc gggcaatctg cccccgaagt
     5581 tcaccgcctg cggcgtcggc caccttgacc catgcctgat agttcttcgg gctggtttcc
     5641 actaccaggg caggctcccg gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg
     5701 tcgtccacca gcaccagacc atgccgctcc tgctcggcgg gcctgatata cacgtcattg
     5761 ccctgggcat tcatccgctt gagccatggc gtgttctgga gcacttcggc ggctgaccat
     5821 tcccggttca tcatctggcc ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag
     5881 cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca
     5941 ccaagcgatt cccacacatt atacgagccg gaagcataaa gtgtagctag atccgaagga
     6001 tgagccgggc tgaatgatcg accgagacag gccctgcggg gctgcacacg cgcccccacc
     6061 cttcgggtag ggggaaaggc cgctaaagcg gctaaaagcg ctccagcgta tttctgcggg
     6121 gtttggtgtg gggtttagcg ggctttgccc gcctttcccc ctgccgcgca gcggtggggc
     6181 ggtgtgtagc ctagcgcagc gaatagacca gctatccggc ctctggccgg gcatattggg
     6241 caagggcagc agcgccccac aagggcgctg ataaccgcgc ctagtggatt attcttagat
     6301 aatcatggat ggatttttcc aacaccccgc cagcccccgc ccctgctggg tttgcaggtt
     6361 tgggggcgtg acagttattg caggggttcg tgacagttat tgcagggggg cgtgacagtt
     6421 attgcagggg ttcgtgacag ttagggcgcg cccagctgtc tagggcggcg gatttgtcct
     6481 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     6541 agcctttcgt tttatttgat gcctttaatt aaacgttcgt aatcaagcca cttccttttt
     6601 gcattgacgc agggtgtcgg aaggcaactc gccgaacgcg ctcctatagt tttcagcgaa
     6661 gcgtcccaaa tgtaagaagc cgtagtctag ggctatctca gttatactac gcacattggc
     6721 actgggatcg ttcaagcagg cgcggatgct ttcgagcttg cggttgcgga tgtagttctt
     6781 cggcgtggtg ccggcgtgct tctcgaacaa attgtagagc gagcgtggac tcatcatcgc
     6841 cagctccgct aaccgctcaa ggctgatatt ccgtttgaga ttctcctcaa tgaattgaac
     6901 gactcgctcg aaagacgggt tacctttgct gaaaatttca cggctgacat tgctgcccag
     6961 catttcgagc agcttggaag cgatgatccc cgcatagtgc tcttggaccc gaggcatcga
     7021 ctttgtatgt tccgcttcgt cacaaactaa cccgagtaga ttgataaagc catcgagttg
     7081 ctggagattg tgtcgcgcgg cgaaacggat accctccctc ggcttgtgcc aattgttgtc
     7141 actgcacgcc cgatcaagga ccactgaggg caatttaacg ataaatttct cgcaatcttc
     7201 tgaataggtc aggtcggctt ggtcatccgg attgagcagc aatagttcgc ccggcgcaaa
     7261 atagtgctcc tggccatggc cacgccacag gcaatggcct ttgagtatta tttgcagatg
     7321 ataacaggtc tctaatccag gcgagattac cctcacgcta ccgccgtagc tgattcgaca
     7381 cagatcgagg catccgaaga ttctgtggtg cagcctgcct gccgggcgcc cgcccttggg
     7441 caggcgaata gagtgcgtac cgacatactg gttaacataa tcggagactg catagggctc
     7501 ggcgtggacg aagatctgac ttttctcgtt caataagcaa aaatccatag ttcacggttc
     7561 tcttatttta atgtgggctg cttggtgtga tgtagaaagg cgccaagtcg atgaaaatgc
     7621 atctcgacgt gatgcgtata cgggttaccc ccattgccac gttgcgccat cctttttgca
     7681 atcagtgacc acttttccaa gcaaaaataa cgccaagcag aacgaagacg ttctttttaa
     7741 gaagcgagaa caccagaagt tcgtgctgtc ggggcatggg gcgacgaatt ggcggataaa
     7801 ggggatctgc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     7861 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgta attacgtatg
     7921 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     7981 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     8041 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     8101 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     8161 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgcatggg
     8221 aattagcttg atctgaccaa cgaccggtag cggagctatc caacggcggt ataccaggaa
     8281 aacacacagc aggtacatca gaacagtacc atgactgaag aacaaatagt tttttcctga
     8341 tccataaagc agaacggcct gctccatgac aaatctggct ccccaactaa tgccccatgc
     8401 agccagcata accagcataa agtgcagtgt ccggtttgat agggataagt ccagccttgc
     8461 aagaagcgga tacaggagtg caaaaaatgg ctatctctag taaggcctac cccttaggct
     8521 ttatgcaacc tctaggatcc gaattcgaag gagatataca tatgactcaa tggaacccca
     8581 acctttttcg taaggaaggt ttatctaaag gatatgattc tcactatttg acacgcttag
     8641 tcgagcaagg ggaaacaatt aatgaaagaa accttcctgt agttttttct ctagcacatt
     8701 tagccaattt atctcatact ttgtattctg acttacacgg ttttgtaagc cgtaacgacc
     8761 ttgaacgaaa agacttccca tataaaaact tttcaatttc gaaaaggacc ggaggaaaac
     8821 gttggatttc cattcctact cctgcactta tggctgtgca gtattggata aacaaaaata
     8881 tacttaatag aattattcct cacgctgcag catatgctta tgtcccgaaa agaaaaataa
     8941 tccagcatgc acaacgtcat tgcggttcag gctggttgat taaaattgat attaaagatt
     9001 tttttggcaa tataagtgag cgccaagttt ttgatgtttt tctaaatcta ggatatccaa
     9061 aactcttatc cttcgaaatg gcaaggttgt gcacacgagt aacccccaaa agaaagggaa
     9121 agaggtggtg taacactaat aatgaacata caatcactgg ttatgattca aaatttgtgg
     9181 gcagcttacc acaaggagca cccacaagcc ctgcattatc gaatctggtt tgtatcggtc
     9241 tcgataagca actaacatcg cttgcaatat ctgaaggcgc tacttacagc cgttatgctg
     9301 acgatatatg tttttcttta ccaatgggat gtagaaaaag cgcattacac atcaaaaaag
     9361 agatatctaa aattcttcat ggatatggtt tttctgataa cagcaaaaaa acgaggctaa
     9421 ttccgccagg ggctagaaaa gttcttactg ggcttaatgt aaataatgac acaccaagaa
     9481 tacctaaaga aataagggat tcagttagaa tgcatctata ttatgcaaaa aaaaatggta
     9541 tcgcaactca ttgtaaaacg cgtggttttc gttcagttat tggattctac aactatctat
     9601 caggagtaat aggatatatt tattccatag actcaaacat ggggaataag ttccggaatg
     9661 aattcaactc attaccttgg gtagattttt ctttataata ccgttcatat gaggctagag
     9721 caggtgattg ataagattcc gtatggggtt ccgtcgtgaa acttagttgt ggtagataca
     9781 tatatagtgt atcaactgcc attggcagcc tacgattccg tttgaatggt ttgacaggat
     9841 gccaacgagc acggattccg tcgctctttg tagtagcctt cgccagagtg agaagatgca
     9901 aattagcgat accggccgca gccacactcc ttcacgccgt cctcgcccgt gaagactggc
     9961 agaaccccct cacttacgtg aggtgaagta agtgactcaa tggaacccca tacggaatct
    10021 tatcagccac ctgcatgatc cgcggccgca ctcgatcgaa ataattttgt ttaactttaa
    10081 ggagg
//
