LOCUS       sequence-48956-e         448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 448)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRDT
FEATURES             Location/Qualifiers
     source          1..448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga atactaagaa aaatgggcga ttgacaaatc aacttcagta tctacaaaaa
      121 gttgtcctaa aggatttatg gaagcatagt ttttcatggc cctttcaacg tcctgtggat
      181 gctgtgaaac tacagttgcc tgattattat accattataa aaaacccaat ggatttaaat
      241 acaattaaga agcgcttgga gaataaatat tatgcgaagg cttcagaatg tatagaagac
      301 ttcaatacaa tgttctcaaa ttgttattta tataacaagc ctggagatga cattgttctt
      361 atggcacaag ctctagagaa gctgtttatg cagaaattat ctcagatgcc acaagaagag
      421 tgacagtaaa ggtggatacg gatccgaa
//
