LOCUS       sequence-48957-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX3
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             604..624
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             643..666
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcaagaga agtcgaagat ggactgccat ggtggcataa gtggcaccat ttacgagtac
       61 ggagccctca ccattgatgg ggaggagtac atccccttca agcagtatgc tggcaaatac
      121 gtcctctttg tcaacgtggc cagctacgga ggcctgacgg gccagtacat tgaactgaat
      181 gcactacagg aagagcttgc accattcggt ctggtcattc tgggctttcc ctgcaaccaa
      241 tttggaaaac aggaaccagg agagaactca gagatccttc ctaccctcaa gtatgtccga
      301 ccaggtggag gctttgtccc taatttccag ctctttgaga aaggggatgt caatggagag
      361 aaagagcaga aattctacac tttcctaaag aactcctgtc ctcccacctc ggagctcctg
      421 ggtacatctg accgcctctt ctgggaaccc atgaaggttc acgacatccg ctggaacttt
      481 gagaagttcc tggtggggcc agatggtata cccatcatgc gctggcacca ccggaccacg
      541 gtcagcaacg tcaagatgga catcctgtcc tacatgaggc ggcaggcagc cctgggggtc
      601 gcagagaacc tctacttcca atcgcaccat catcaccacc atgattacaa ggatgacgac
      661 gataagtgag gatcc
//
