LOCUS       sequence-489575-        9328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Klf5 epicenter-driven fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Ge Y, Gomez NC, Adam RC, Nikolova M, Yang H, Verma A, Lu CP, Polak 
            L, Yuan S, Elemento O, Fuchs E
  TITLE     Stem Cell Lineage Infidelity Drives Wound Repair and Cancer.
  JOURNAL   Cell. 2017 May 4;169(4):636-650.e14. doi: 
            10.1016/j.cell.2017.03.042. Epub 2017 Apr 20.
  PUBMED    28434617
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03.042"; journalName: "Cell"; date: "2017-05-4-
            4"; volume: "169"; issue: "4"; pages: "636-650.e14"
COMMENT     Sequence Label: Klf5-eGFP-PGK-H2BRFP
FEATURES             Location/Qualifiers
     source          1..9328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    103..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(114..133)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     168..187
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             296..529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        1450..1646
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1497..1632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1668..1677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1674..1676,1677..1679,1680..2393)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1674..1676,1677..1679,1680..2390)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1719..1740)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1980..1999)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2327..2348
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(2438..3115)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(2438..3115)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2500..2523)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2698..2717)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2967..2985
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3112..3121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3134..3511)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(3523..4033)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3586..3604)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4082..4199
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(4295..4543)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(4353..4372)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(4523..4543)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(4841..4885)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5070..5303
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5796..5921
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5968..6148
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6149..6375)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6403..6423)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6403..6421)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6442..6458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6442..6458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6455..6477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6466..6482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6490..6496,6497..6514,6515..6520))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6535..6556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6673..6690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6844..7432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6924..6943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7603..8394,8395..8463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8226..8245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8464..8568)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8594..9049)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8731..8752)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8943..8962
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9176..9198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9190..9207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9191..9207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9217..9236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9217..9235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(9256..9328,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
      121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      181 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
      241 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
      301 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      361 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      421 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      481 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      541 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
      601 tggctttgtc taaatgactg gaaatgatac cataaaactc tatggatatc cttgtattgg
      661 ttttagatag caatggattt cctgattctt gtaccagggt gcttgggtga atctccccag
      721 cacactacct ctagctgtga ccttatcaga tttcaggagc tgagcaggct ttgtctgggg
      781 ccgtttgtgc gtggcagtgg tccaaggcaa accaggtgct acaggaagtg gacttggtga
      841 ttcagtcatg tgcattcttt cttatgattc agtctgaccc actcactaat caccgtgagt
      901 gggcattctg ctttcagggg tgttcatgaa taagatgcat ggtaaaggtc ttaagcattt
      961 gtgctgacag agagctttca tggagatttg ctctatctta ggttgtcaga gttgcaacag
     1021 acaggatcag ttctgttgtc gcaataaatg ctgaaggtaa taccacttta ggtatgctat
     1081 atgctccatt tcctgtcttt ggtcaacaca gcagcacagg atacgttact aggcacccgg
     1141 ggctttttac ttcagagcta aatttacatt tggctgtaca tgtactttgt aaaagaaact
     1201 gttaaattgt tggacattaa agtaattaat atatgtttgt gcatttgtac ctctatgaat
     1261 ctatctatga aaccatggct tatttcatct gtttatattt tccctgaagg cttttaagac
     1321 tatcaaactg aagcaatggc acaatagatt agaaactgaa gcaagaagta aatactacca
     1381 agaaagaaag gcagatggga gctgaggagt ctgtacttgc tgaagtacat aagattgtgt
     1441 ggatgtgcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     1501 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     1561 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     1621 tttttggagg cctaggcttt tgcaaaaagc tgggggatcc accggtcgcc accatggtga
     1681 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1741 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1801 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1861 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1921 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1981 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2041 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2101 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2161 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2221 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2281 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2341 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     2401 gcgactctag aggatcctca cgtcgactag tctcgagtta ggcgccggtg gagtggcggc
     2461 cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     2521 gcttgatgtc ggtcttgtag gcgccgggca gctgcacggg cttcttggcc atgtaggtgg
     2581 tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt cagcctcatc ttgatctcgc
     2641 ccttcagggc gccgtcctcg gggtacatcc gctcggtgga ggcctcccag cccatggtct
     2701 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
     2761 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     2821 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
     2881 tgtcggcggg gtgcttcacg taggccttgg agccgtactg gaactgaggg gacaggatgt
     2941 cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct
     3001 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     3061 ccatgcgcac cttgaagcgc atgaactcct tgatgacgtc ctcggaggag gccatggtgg
     3121 cgaccggtgg atccttagcg ctggtgtact tggtgatggc cttagtaccc tcggacacgg
     3181 cgtgcttggc caactcccca ggcagcagca ggcgcacggc cgtctggatc tccctggagg
     3241 tgatggtcga gcgcttgttg taatgcgcca ggcgggaagc ctcacctgcg atgcgctcga
     3301 aaatgtcgtt cacaaacgag ttcatgatgc ccatggcctt ggacgaaatg ccggtgtcag
     3361 ggtggacctg cttcagaacc ttgtacacat agatggaata gctctccttg cggctgcgct
     3421 tgcgcttctt gccgcctttc ttctgcgcct tagtcaccgc cttcttggag ccctttttcg
     3481 gggcgggagc agacttcgct ggctctggca tggtagatcc ccctggggag agaggtcggt
     3541 gattcggtca acgagggagc cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga
     3601 acaccgcgcg ggcaggaaca gggcccacac taccgcccca caccccgcct cccgcaccgc
     3661 cccttcccgg ccgctgctct cggcgcgccc tgctgagcag ccgctattgg ccacagccca
     3721 tcgcggtcgg cgcgctgcca ttgctccctg gcgctgtccg tctgcgaggg tactagtgag
     3781 acgtgcggct tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac cttcccgact
     3841 taggggcgga gcaggaagcg tcgccggggg gcccacaagg gtagcggcga agatccgggt
     3901 gacgctgcga acggacgtga agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc
     3961 ggaaccacgc ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg
     4021 caaccccaac cccctcgagc cgcggccaaa gtggatctct gctgtccctg taataaaccc
     4081 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     4141 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
     4201 tgtggatgaa tactgccatt tgtctcgagg tcgagaatta aaaacaacaa gatgaagagc
     4261 accaactcga gttggtgctc ttcatcttgt tgccggtgtt tcgtcctttc cacaagatat
     4321 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
     4381 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
     4441 gtactaatat ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt
     4501 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcggtgaag ggggcggccg
     4561 ctcgaggcta gtctcgtgat cgataccgtc gagatccgtt cactaatcga atggatctgt
     4621 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     4681 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     4741 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     4801 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     4861 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     4921 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     4981 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     5041 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     5101 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     5161 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     5221 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     5281 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     5341 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     5401 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
     5461 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
     5521 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
     5581 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
     5641 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
     5701 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
     5761 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
     5821 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     5881 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     5941 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     6001 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     6061 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     6121 ctcagatctg gtctaaccag agagacccgt ttatgtatcg agctaggcac ttaaatacaa
     6181 tatctctgca atgcggcaat tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc
     6241 ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt
     6301 tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagagta
     6361 ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt gagggttaat
     6421 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8581 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     8641 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     8701 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     8761 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     8821 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     8881 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     8941 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     9001 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     9061 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9121 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9181 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     9241 ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     9301 ccgaggcggc ctcggcctct gcataaat
//
