LOCUS       sequence-489576-        9773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inner root sheath (IRS)-specific Cux1 epicenter-driven fluorescent 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Adam RC, Yang H, Rockowitz S, Larsen SB, Nikolova M, Oristian DS, 
            Polak L, Kadaja M, Asare A, Zheng D, Fuchs E
  TITLE     Pioneer factors govern super-enhancer dynamics in stem cell 
            plasticity and lineage choice.
  JOURNAL   Nature. 2015 May 21;521(7552):366-70. doi: 10.1038/nature14289. Epub
            2015 Mar 18.
  PUBMED    25799994
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14289"; journalName: "Nature"; date: "2015-05-21- 
            21"; volume: "521"; issue: "7552"; pages: "366-70"
COMMENT     Sequence Label: Cux1-SE(IRS)-eGFP-PGK-H2BRFP
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1056..1289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1361..1482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1398..1417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1452..1471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1522..1657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1677..1696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1678..1696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1706..1723)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1706..1722)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1715..1737)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1864..2319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1951..1970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2161..2182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2345..2449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2450..2518,2519..3310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2668..2687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3481..4069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3970..3989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4223..4240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4357..4378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4393..4398,4399..4416,4417..4423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4431..4447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4436..4458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4455..4471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4455..4471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4490..4510
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4492..4510
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4538..4764
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4765..4945
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4992..5117
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5610..5843
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6028..6072
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6370..6618
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6370..6390
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6541..6560
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6714..6831
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6880..7390
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7309..7327
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7402..7779
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      7792..7801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7798..8475
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             7798..8475
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(7928..7946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8196..8215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8390..8413
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             complement(join(8520..9233,9234..9236,9237..9239))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8523..9233,9234..9236,9237..9239))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8565..8586)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8914..8933
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9173..9194
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9236..9245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9267..9463)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9281..9416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatc aaccccccct caaacttacc ccccaaataa acaacaataa aaactaacga
       61 gatcagaagt gattctttct catcgttttc cctacctacc tacccttgtg ggatactgtg
      121 agccgggaga cacttgtatc atgtcctcca agcctgatag aatcttccag agtggagagt
      181 gagatgggct tggagttttg agtgatggtt cggagagatt ggatttatct gcctcccatc
      241 gcccattctc agtaataagc gaagctgcag aaagcccttg gcccgagata taatgtgtca
      301 tgtaattact gtaataatac tttgtacttc tgcagagcct ttcatctgcg gtgctccacg
      361 gcctttataa acattaatta attcccataa ctgggcgatg aggtaggtaa gtatcgtcat
      421 tcccatttca cagatgagaa gagccagcac acgggagggt gggccaccag ccttcagttt
      481 cttggcaaag cccaccgaca cagccagcgc ccactgggag tggtgtccat ttaaacttgg
      541 tattttttcc aggctgccag gcctagtctc ttactgtata aggactacac aagccccagg
      601 tgtcctttac atactctttg ggggacaagc agtagcccta ctcttctccc ctcctgtgga
      661 acacccacat ccaagcccta ggcacaggga ggtctgtgat ggcctctgga tccctccatt
      721 agggaggcag ggatcttgca gaatcaaggt cctagaaaag acagatgtct aaatcaagtt
      781 taagcctctc cccctgttag acaagtcaaa ccctgctctg acttcctttc tataatgtgc
      841 ccccaaggaa tggtggccta gacccctctt gctaacatct gtatcccatc ccaaaccaca
      901 atgaattgat aagctctctc atatacatta acccttttta ccgctacctc tctaacctgg
      961 gacatccatc aaaccaagac cacaggtacc tttaagacca atgacttaca aggcagctgt
     1021 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     1081 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     1141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1261 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     1321 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     1381 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1441 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     1501 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1621 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1681 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1741 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1801 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1861 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1921 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2041 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2161 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2221 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2281 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     2341 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2401 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2461 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2521 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2581 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2641 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2701 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2761 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2821 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2881 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2941 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3001 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3061 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3121 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3181 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3241 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3301 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3601 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4141 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4261 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4321 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4381 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4441 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     4501 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     4561 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     4621 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4681 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4741 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4801 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4861 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4921 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4981 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     5041 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     5101 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     5161 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     5221 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     5281 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     5341 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     5401 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     5461 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     5521 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     5581 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5641 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5701 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5761 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5821 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5881 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5941 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     6001 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     6061 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     6121 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     6181 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     6241 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     6301 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc
     6361 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     6421 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     6481 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     6541 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     6601 tggaaaggac gaaacaccgg caacaagatg aagagcacca actcgagttg gtgctcttca
     6661 tcttgttgtt tttaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     6721 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     6781 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     6841 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     6901 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     6961 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     7021 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     7081 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     7141 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     7201 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     7261 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     7321 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     7381 ctctccccag ggggatctac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag
     7441 ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc
     7501 cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc
     7561 ggcatttcgt ccaaggccat gggcatcatg aactcgtttg tgaacgacat tttcgagcgc
     7621 atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg
     7681 gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc
     7741 gagggtacta aggccatcac caagtacacc agcgctaagg atccaccggt cgccaccatg
     7801 gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
     7861 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     7921 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     7981 tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     8041 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     8101 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     8161 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     8221 ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     8281 aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
     8341 atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc
     8401 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
     8461 tccaccggcg cctaactcga gactagtcga cgtgaggatc ctctagagtc gcggccgctt
     8521 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     8581 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     8641 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     8701 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     8761 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     8821 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     8881 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     8941 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     9001 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     9061 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     9121 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     9181 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     9241 gtggcgaccg gtggatcccc cagctttttg caaaagccta ggcctccaaa aaagcctcct
     9301 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     9361 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     9421 ggagttaggg gcgggactat ggttgctgac taattgagat gcacatcaga ggatcaggct
     9481 ggtgatgtgg ccctgggggt agactgcttg ccttccaagt gggaagccca ggttctatcc
     9541 tcaaatgaac caggggtcat gtgctatgtg ctatagaccc agaaattggg aggtggagac
     9601 aggatgatca aaagttgaag gctatccttg gctacatagg cagtttaagt ttgaggctag
     9661 actaggctat atgagacccc tccccctttc tctctctctc tctctcttaa aaacaaaaca
     9721 aaccagaaca aaccgaaaaa acaaaaataa gatcccaaga caaaacaaaa aac
//
