LOCUS       sequence-489589-       11396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, 
            and Pex22 peroxisomal chauffeur.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11396)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1337
FEATURES             Location/Qualifiers
     source          1..11396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5366..5553
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5421..5445)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5897..6310
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6444..7151
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             7669..8472
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9114..9929)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9114..9929)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9837..9856
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9921..9940
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10154..10742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10643..10662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgtggaac tcactaaaag cattcgctct agtgttcgga ggttgttgct
      781 ccaatgttat tacatttgaa accttaatga gcaacgaaac aggcagcatt aataatctca
      841 ttactttttg tcaattttta tttgttacat gtcagggact ccccgaattt ctggatgttc
      901 atcaaccttt cccttacttc aagcctctga aaactccact tcacgtttat gtcattactg
      961 tcgttttatt ttatatttca tctacgacga ataataacgt atttaaatac aacatatcca
     1021 ttccgattca tatcgttttc agatgcttcg gaaccgtaat aaccatgttt acatgctggt
     1081 tattaaatgg taggaaatat acaaagattc agattttatc aacactattt ttaaccattg
     1141 gagctataat tgcctcatta ttcaaagatg ctgattttcg gtaccaagat ttgaaactgc
     1201 aagcgtggaa aattggaagt gaccaatctg tagatctcac ttttattttt ggcatttgta
     1261 tactagtact ttcatcattc acatcatctt tactttcagc atacaatgaa cgtacgtatc
     1321 aaaaatatgg aaagcattgg aaggagaata tcttttacag tcatttttta tcattacctc
     1381 tctttctatt tagtcgaaag cagttaattc acgaatatcg agtgatgaga aagtctgaaa
     1441 gaatattgtg ttcaaatttt ggagggaaga ttctagtccc gcgagaagaa actctccttc
     1501 ttttcaatgt gttaacacaa tatttttgcg tcaagggtgt taatatcctc gccagtaaaa
     1561 caaacgctct gacactttcc ataacattac ttgtaagaaa atttataagt cttttactga
     1621 gcgtccgact tttcgataat aacttgtcat atactggcta catcggggtc tacctagtct
     1681 tttttggtgc ctttatatac tcgctgggat caattcaccc caggcaaaat gacaaggggg
     1741 cgataaagaa aagtaaaggt tctgtttcta aaggtgaaga attattcact ggtgttgtcc
     1801 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
     1861 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
     1921 tgccagttcc atggccaacc ttagtcacta ctttatcttg gggtgttcaa tgttttgcaa
     1981 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
     2041 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
     2101 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
     2161 atggtaacat tttaggtcac aaattggaat acaattattt ctctgacaat gtttacatca
     2221 ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa aattagacac aacattgaag
     2281 atggtggtgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
     2341 tcttgttacc agacaaccat tacttatcca ctcaatctaa gttatccaaa gatccaaacg
     2401 aaaagaggga ccacatggtc ttgttagaat ttgttactgc tgctggtatt accttgggta
     2461 tggatgaatt gtacaaagga tccgcagagt atgtgcgggc cctgtttgac tttaatggga
     2521 atgatgaaga agaccttccc tttaagaaag gagacatcct gagaatccgg gataagcctg
     2581 aagagcagtg gtggaatgca gaggacagcg aaggaaagag ggggatgatt cctgtccctt
     2641 acgtggagaa gtatggatct gggagcggat ctggaggcag catggcggaa tatgtacgag
     2701 ctctttttga ttttaatggc aatgatgagg aggatctccc ttttaaaaag ggcgatattc
     2761 tgcgcattcg tgataagcca gaggaacaat ggtggaacgc cgaggatagt gaggggaaac
     2821 gcggcatgat tccggtgccg tatgtagaga agtactaatg gcgccaattg gtgcggcaat
     2881 tgataataac gaaaatgtct tttaatgatc tgggtataat gaggaatttt ccgaacgttt
     2941 ttactttata tatatatata catgtaacat atattctata cgctatagag aaaggaaatt
     3001 tttcaattaa aaaaaaaata gagaaagagt ttcacttctt gattatcgct aacactaatg
     3061 gttgaagtac tgctacttta attttatgct gccaaaccag atgtcaacac agctacaacg
     3121 tatatattaa atttgctctt gttctgtact ttcctaattc ttatgtaaaa agacaagaat
     3181 ttatgatact atttaataac aaaaaactac ctaagaaaag catcatgcag tcgaaattga
     3241 aatcgaaaag taaaacttta acggaacatg tttgaaattc taagaaagca tacatcttca
     3301 tcccttatat atagagttat gtttgatatt agtagtcatg ttgtaatctc tggcctaagt
     3361 atacgtaacg aaaatggtag cacgtcgcgt ttatggcccc caggttaatg tgttctctga
     3421 aattcgcatc actttgagaa ataatgggaa caccttacgc gtgagctgtg cccaccgctt
     3481 cgcctaataa agcggtgttc tcaaaatttc tccccgtttt caggatcacg agcgccatct
     3541 agttctggta aaatcgcgct tacaagaaca aagaaaagaa acatcgcgta atgcaacagt
     3601 gagacacttg ccgtcatata taaggttttg gatcagtaac cgttatttga gcataacaca
     3661 ggtttttaaa tatattatta tatatcatgg tatatgtgta aaattttttt gctgactggt
     3721 tttgtttatt tatttagctt tttaaaaatt ttactttctt cttgttaatt ttttctgatt
     3781 gctctatact caaaccaaca acaacttact ctacaactaa gatctatgct catagaaaac
     3841 actaatgatc ggtttggtat cgtcatagat gcagggtctt cgggttcgag aatccatgtg
     3901 tttaagtggc aggacacgga atcgcttctt catgcaacaa accaggactc acagtccata
     3961 ttacaatcag tacctcatat tcatcaagaa aaagactgga cttttaagct gaatccaggc
     4021 ttgtcgagtt ttgaaaaaaa acctcaagat gcgtacaaat ctcacatcaa gccgttacta
     4081 gattttgcta agaatattat ccctgaatca cattggtcga gttgtcccgt ttttattcaa
     4141 gcaaccgcgg gcatgcgcct tttacctcaa gacatacaat cttccatttt ggatggtttg
     4201 tgtcaggggc tcaaacaccc tgcagaattt ttggttgagg attgctcagc acaaattcaa
     4261 gtcattgatg gtgaaaccga aggtttatat ggctggcttg gcttaaacta tctatatgga
     4321 cactttaatg actataatcc agaagtctct gaccatttta catttgggtt tatggacatg
     4381 ggcggtgcct ctactcagat tgcgtttgca ccgcatgatt caggagaaat agctagacat
     4441 agagatgaca tcgccaccat cttcttgagg agtgttaacg gagatttgca gaaatgggac
     4501 gtttttgtta gtacgtggtt agggtttggt gccaatcagg ctagaagaag gtacttggct
     4561 cagttgatca atacccttcc agaaaacaca aatgattacg aaaatgatga cttctctaca
     4621 aggaacttga atgatccatg tatgccgcga ggaagcagca cggattttga atttaaagat
     4681 accatatttc acatcgcggg ttctgggaat tacgaacaat gtacaaaatc tatttatccg
     4741 ttacttttaa agaacatgcc ttgtgatgac gagccgtgct tgtttaatgg tgttcatgct
     4801 cctcgaatag actttgccaa tgacaaattt ataggtactt ctgagtactg gtacactgcc
     4861 aacgacgtat tcaaacttgg gggcgaatac aactttgaca aatttagcaa aagcctaagg
     4921 gagttttgca attccaattg gacgcaaata ttagcgaaca gtgataaagg cgtgtataat
     4981 tctattccgg agaatttttt gaaagatgca tgctttaagg gcaattgggt ccttaatata
     5041 cttcatgaag ggtttgatat gcctcgaata gatgtcgacg cagaaaatgt caatgacagg
     5101 cccttatttc aaagtgtgga aaaagtcgaa gaacgagagt tatcgtggac actaggcaga
     5161 attttacttt atgcctcagg tagcatattg gcaggtaatg atgacttcat ggtgggtatt
     5221 gcgcccagtg aaagaagaac taagctcact ggtaagaaat tcatacctgg caagttactg
     5281 gagtctgatc aactacgcaa gcaaagttcc ggatccggtt cattgggaag aggtagaaga
     5341 tccaaattgt aataactcga gtggcgcgaa tttcttatga tttatgattt ttattattaa
     5401 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     5461 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
     5521 tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc tgcaaatcgc
     5581 tccccatttc gctggatgca cacactggct taagatgaca acgccttgcc aacagggagt
     5641 tcttcagaga catggaggct caaaacgaaa ttattgacag cctagacatc aatagtcata
     5701 caacagaaag cgaccaccca actttggctg ataatagcgt ataaacaatg catactttgt
     5761 acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata catatcacat
     5821 aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct tacatcacac
     5881 ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct actccttttt
     5941 tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa cacccaagca
     6001 cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc cgtactaaag
     6061 gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa
     6121 aatttttatc acgtttcttt ttcttgaaaa tttttttttt tgattttttt ctctttcgat
     6181 gacctcccat tgatatttaa gttaataaac ggtcatcaat ttctcaagtt tcagtttcat
     6241 ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa gcatagcaat
     6301 ctaatctaag ttttaattac aaaagatcta tgccaccacc atcaagaagt agaataaaca
     6361 aaacaagaac attaggaata gtgggtacag ctatagcagt gttggtcacg tcctactata
     6421 tatatcaaaa ggtgacaggt tctgtgtcca aaggagagga gttaatcaag gaaaacatga
     6481 gaatgaaagt tgtcatggag ggctccgtta atggtcacca attcaagtgt acaggggaag
     6541 gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat taaagtaatt gaaggcggac
     6601 cactaccatt tgcatttgac attctggcaa cgtcattcat gtacggatca cgaactttca
     6661 tcaagtaccc taaaggtata ccagactttt tcaagcaatc ttttccagag ggttttacat
     6721 gggaaagggt tacaagatac gaagatgggg gtgtcgtcac agttatgcaa gatacttcat
     6781 tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg ggtgaatttt ccttctaacg
     6841 gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa tactgaaatg atgtacccag
     6901 ctgatggagg tttgagaggc tacacacaca tggcgcttaa agttgatggt ggaggtcatt
     6961 tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac tgttggcaat atcaaaatgc
     7021 caggaataca tgctgtagac cacagactag aaagactcga agagagcgat aacgaaatgt
     7081 tcgttgtaca gagagagcat gccgtagcca aatttgctgg cttaggcggt ggtatggatg
     7141 aattgtataa gggatccggg tcaggctccg gatctggccc tcccccagct cttccgccaa
     7201 aaagacgaag gtaatggcag tgcttttaac taagaattat tagtcttttc tgcttatttt
     7261 ttcatcatag tttagaacac tttatattaa cgaatagttt atgaatctat ttaggtttaa
     7321 aaattgatac agttttataa gttacttttt caaagactcg tgctgtctat tgcataatgc
     7381 actggaaggg gaaaaaaaag gtgcacacgc gtggcttttt cttgaatttg cagtttgaaa
     7441 aatgctgagc agttacagag atgttacgaa ccactagtgc actgcagtac acggtttcct
     7501 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     7561 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     7621 ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct
     7681 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     7741 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     7801 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     7861 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     7921 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
     7981 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     8041 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     8101 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     8161 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     8221 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     8281 ggtttagatg acaagggaga tgcattgggt caacagtata gaaccgtgga tgatgtggtt
     8341 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     8401 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     8461 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     8521 gcttcaattt aattatatca gttattaccc gagtagagca cttgaatcca ctgccccggg
     8581 aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
     8641 catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa cagtatttta
     8701 cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcatt gaactccaaa
     8761 gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt catgcgtttg
     8821 tactctaatc tggtagaaag atgtttcaca gactgtgtca atgacttcac aacatcaaag
     8881 ctaaccaata aggaacaaac atgcatcatg aagtgctcag aaaagttctt gaagcatagc
     8941 gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg cttgggccga
     9001 taaggtgtac tggcgtatat atatctaatt atgtatctct ggtgtagccc atttttagca
     9061 tgtaaatata aagaccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa
     9121 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     9181 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     9241 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     9301 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     9361 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     9421 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     9481 gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     9541 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     9601 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     9661 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     9721 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     9781 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     9841 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag
     9901 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     9961 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
    10021 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca
    10081 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10141 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10201 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10261 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10321 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10381 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10441 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10501 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10561 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10621 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10681 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10741 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10801 tgttctttcc tgcgttatcc cctgattctg tggataaccg tgcggccgcc aatgctaaat
    10861 tcgagtgaaa cacaggaaga tcagaaaatc ctcatttcat ccatattaac aataatttca
    10921 aatgtttatt tgcattattt gaaactaggc aagacaagca acgaaacgtt tttgaaaatt
    10981 ttgagtattt tcaataaatt tgtagaggac tcagatattg aaaaaaagct acagcaatta
    11041 atacttgata agaagagtat tgagaagggc aacggttcat catctcatgg atctgcacat
    11101 gaacaaacac cagagtcaaa cgacgttgaa attgaggcta ctgcgccaat tgatgacaat
    11161 acagacgatg ataacaaacc gaagttatct gatgtagaaa aggattaaag atgctaagag
    11221 atagtgatga tatttcataa ataatgtaat tctatatatg ttaattacct tttttgcgag
    11281 gcatatttat ggtgaaggat aagttttgac catcaaagaa ggttaatgtg gctgtggttt
    11341 cagggtccat aaagcccaca tggataacat tacccctgaa ttcgcatcta gactga
//
