LOCUS       sequence-489599-        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For exogenous expression of codon optimized BLTP3A (UHRF1BP1) as a 
            C-terminal fusion to EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Hanna MG, Rodriguez Cruz HO, Fujise K, Wu Y, Xu CS, Pang S, Li Z, 
            Monetti M, De Camilli P
  TITLE     BLTP3A is associated with membranes of the late endocytic pathway 
            and is an effector of CASM.
  JOURNAL   EMBO J (2025) 44: 6168 - 6195
  PUBMED    39386594
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J 
            (2025) 44: 6168 - 6195"
COMMENT     Sequence Label: pEGFP-N1 BLTP3A
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5012..5021
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5018..5020,5021..5023,5024..5737)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5018..5020,5021..5023,5024..5734)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5063..5084)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5324..5343)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5671..5692
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5860..5981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5897..5916)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5951..5970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5988..6443)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6125..6146)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6337..6356
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6470..6574
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6576..6933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6576..6767
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6599..6619)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6784..6919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6968..7762
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6968..7762
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7022..7041)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7632..7651
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7950..7969)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7994..8041
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8370..8958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8859..8878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagac cggtcgccac catggcgggt atcatcaaaa aacaaatcct
      661 gaaacacctg agccgcttta ccaagaacct gagcccagac aaaatcaacc tgagcaccct
      721 gaaaggcgag ggtcagctga ccaacctgga gctggacgag gaagtgctgc agaacgtgct
      781 ggagctgccg acctggctgg ccattacccg cgtgtactgc aaccgtgcga gcatccgcat
      841 tcagtggacc aagctgaaaa cccacccgat ctgcctgtgc ctggataagg tggaggtgga
      901 aatgaaaacc tgcgaagacc cgcgtccgcc aaacggtcag agcccaattg cgctggctag
      961 cggtcagagc gagtacggtt ttgccgaaaa ggtggtggag ggcatgttca tcattgtgaa
     1021 cagcatcacc attaagatcc acagcaaagc tttccacgcc agctttgaac tgtggcagct
     1081 gcagggctat agcgtgaacc cgaactggca gcagagcgat ctgcgtctga cccgtatcac
     1141 cgacccgtgc cgtggtgaag tgctgacctt taaggagatt acctggcaga ccctgcgtat
     1201 cgaggccgac gcgaccgata acggcgacca ggacccggtt accaccccac tgcgcctgat
     1261 taccaaccag ggtcgtattc agatcgccct gaagcgtcgc accaaagatt gcaacgtgat
     1321 cagcagcaaa ctgatgttcc tgctggacga tctgctgtgg gtgctgaccg acagccagct
     1381 gaaggccatg atgaaatacg cggaaagcct gagcgaggct atggaaaaga gcgcccacca
     1441 gcgtaaaagc ctggctccag agccggtgca gatcacccca ccagctccaa gcgcccagca
     1501 gagctgggct caggccttcg gcggtagcca gggcaacagc aacagcagca gcagccgcct
     1561 gagccagtac ttcgaaaagt ttgatgtgaa agagagcagc tatcacctgc tgattagccg
     1621 tctggacctg cacatctgcg acgatagcca gagccgtgag ccaggtgtga gcgccaaccg
     1681 tctgatgggc ggtgcgatgc agctgacctt ccgtaagatg gcttttgatt actatccgtt
     1741 ccactgggcc ggcgacagct gcaaacactg ggtgcgccac tgcgaggcta tggaaacccg
     1801 tggccagtgg gcccagaagc tggtgatgga atttcagagc aagatggaga aatggcacga
     1861 ggaaaccggc ctgaaaccac catggcacct gggtgtggat agcctgttcc gtcgtaaggc
     1921 tgacagcctg agcagcccgc gtaaaaaccc actggagcgc agcccgagcc agggccgtca
     1981 gccagctttc cagccaccag cttggaaccg tctgcgcagc agctgcatgg tggtgcgcgt
     2041 ggacgatctg gatattcacc aggtgagcac cgctggtcag ccgagcaaga aaccgagcac
     2101 cctgctgagc tgcagccgta agctgcacaa cctgccgacc caggtgagcg cgattcacat
     2161 cgagtttacc gaatactatt tcccggacaa ccaggaactg ccagtgccgt gcccgaacct
     2221 gtacatccag ctgaacggcc tgacctttac catggacccg gtgagcctgc tgtggggtaa
     2281 cctgttttgc ctggacctgt accgtagcct ggagcagttc aaggccattt ataaactgga
     2341 agatagcagc cagaaagacg agcacctgga tatccgcctg gacgcttttt ggctgaaggt
     2401 gagcttcccg ctggagaaac gtgaacgtgc tgagctgcac cgtccacagg ccctggtgtt
     2461 cagcgcgagc ggtatgatcg ctaccaacac ccgtcacgct ccacactgca gctgcagcga
     2521 tctgcagagc ctgttccgtg gttttgccgc ggctgagttc tttcacagca actatgacca
     2581 ctttccgaag gtgccgggcg gtttcagcct gctgcacatg ctgtttctgc accacgcctt
     2641 ccagatggac agctgcctgc cacagccaaa caccctgcca ccacagcgtc cgaaggcgag
     2701 ctgggatctg tggagcgtgc actttaccca gattagcctg gactttgaag gcaccgagaa
     2761 cttcaaaggt cacaccctga acttcgtggc gccgtttccg ctgagcatct gggcttgcct
     2821 gccactgcgt tggcagcagg ctcaggctcg taagctgctg ctggctagcg agggccgcct
     2881 gaaaccaagc gccagcttcg gtagcccggt gcagagcgaa gccctggctc cagatagcat
     2941 gagccaccca cgtagcaaga ccgagcacga cctgaaaagc ctgagcggcc tgaccgaagt
     3001 gatggagatc ctgaaggagg gcagcagcgg tatggacaac aaaggtccgc tgaccgagct
     3061 ggaggacgtg gccgatgtgc acatgctggt gcacagccca gctcacgtgc gtgtgcgcct
     3121 ggatcactac cagtatctgg ccctgctgcg cctgaaggaa gtgctgcagc gtctgcagga
     3181 gcagctgacc aaagacaccg aaagcatgac cggcagcccg ctgcagaacc agaccgcgtg
     3241 cattggtgtg ctgttcccaa gcgccgaggt ggcgctgctg atgcacccag ctccaggtgc
     3301 tgtggacgct gatagcgccg gtagcgatag caccagcctg gtggacagcg aactgagccc
     3361 gagcgaagac cgcgagctga agagcgatgc tagcagcgac cagggtccag ctagcccaga
     3421 gaaagtgctg gaggaaagca gcatcgagaa ccaggatgtg agccaggaac gtccgcacag
     3481 caacggcgag ctgcaggaca gcggtccact ggcgcagcag ctggctggca agggtcacga
     3541 agcggtggag agcctgcagg ctaagaaact gagccgcacc caggccagca gcagcccagc
     3601 cgcgctgaag ccaccagctg gtcgtgaaac cgcggtgaac ggccagggcg agctgattcc
     3661 gctgaaaaac atcgagggtg aactgagcag cgcgattcac atgaccaagg atgctaccaa
     3721 agaagccctg cacgcgacca tggacctgac caaggaggcc gtgagcctga ccaaagatgc
     3781 gttcagcctg ggccgtgacc gcatgaccag caccatgcac aagatgctga gcctgccgcc
     3841 ggctaaggaa ccgatggcca aaaccgatga gggtgtggct gctccggtga gcggtggtgc
     3901 tgctcgtctg cgcttcttta gcatgaaacg caccgtgagc cagcagagct ttgatggcgt
     3961 gagcctggac agcagcggtc cggaagatcg tatcagcgtg gacagcgatg gcagcgacag
     4021 cttcgtgatg ctgctggaga gcgaaagcgg tccagagagc gtgccaccag gtagcctgag
     4081 caacgtgagc gacaacgctg gtgtgcaggg tagcccgctg gtgaacaact acggtcaggg
     4141 tagcccggct gctaacagca gcgtgagccc aagcggtgaa gatctgattt ttcacccggt
     4201 gagcgtgctg gtgctgaagg tgaacgaggt gagcttcggc atcgaagtgc gtggcgagga
     4261 cctgaccgtg gctctgcagg ccgaggaact gaccctgcag cagctgggca ccgtgggtct
     4321 gtggcagttt ctgcacggcc agtgcccggg tacctgcttc caggaaagca gcaccctgaa
     4381 aaccggtcac atccgtccag ctgtgggtct gcgttttgaa gtgggtccag gtgctgctgt
     4441 gcacagccca ctggctagcc agaacggctt cctgcacctg ctgctgcacg gttgcgatct
     4501 ggagctgctg accagcgtgc tgagcggcct gggtccgttt ctggaagacg aggaaattcc
     4561 ggtggtggtg ccgatgcaga ttgagctgct gaacagcagc atcaccctga aggacgatat
     4621 tccaccaatc tatccgacca gcccaggtcc aattccaatc accctggcca tggagcacgt
     4681 ggtgctgaaa cgcagcgacg atggcgtgtt ccacatcggt gctgctgctc aggacaagcc
     4741 aagcgcggaa gtgctgaaga gcgagaaacg tcagccgccg aaagaacagg tgtttctggt
     4801 gccgaccggt gaagtgttcg agcagcaggt gaaggagctg ccgattctgc agaaggaact
     4861 gatcgaaacc aaacaggctc tggccaacgc gaaccaggac aaggaaaaac tgctgcaaga
     4921 gattcgtaag tataacccat tctttgaact gggtggatct ggaggtcgaa ttctgcagtc
     4981 gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     5041 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5101 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5161 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     5221 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5281 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5341 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5401 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     5461 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     5521 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     5581 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     5641 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5701 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata
     5761 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     5821 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     5881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5941 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     6001 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6061 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6121 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6181 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6241 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6301 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6361 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6421 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6541 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     6601 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6661 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6721 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6781 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6901 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     6961 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7021 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7081 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7141 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7201 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7261 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7321 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7381 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     7441 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     7501 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     7561 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7621 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7681 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7741 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     7801 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7921 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     7981 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     8041 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     8101 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     8161 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     8221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9061 accgccatgc at
//
