LOCUS       sequence-489601-       11348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, 
            and Pex22 peroxisomal chauffeur.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11348)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1338
FEATURES             Location/Qualifiers
     source          1..11348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5318..5505
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5373..5397)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5849..6262
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6396..7103
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             7621..8424
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9066..9881)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9066..9881)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9789..9808
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9873..9892
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10106..10694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10595..10614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatggaggtt cacggttcag gattcagaag aatattatta ttggcattat
      781 gtatttcagg tatttggtca gcatatatct accaaggtgt tttacaagaa accttatcaa
      841 caaagagatt tggtcctgat gagaagagat ttgaacattt agcattcttg aatttggccc
      901 aatctgtcgt ttgtttgatt tggtcttata ttatgatcaa gttatggtca aacgctggta
      961 atggtggagc tccatggtgg acttattggt cagccggtat aaccaacacc ataggaccag
     1021 ctatgggtat tgaggcatta aaatatatat cttacccagc acaagtttta gcaaaatcat
     1081 ctaaaatgat accagttatg ttaatgggaa ccttggttta cggaataaga tatacatttc
     1141 cagaatacat gtgcactttc ttggttgcag gtggtgtctc tatatttgca ttgttaaaga
     1201 cctcttctaa gactatctct aagttggcac atcctaatgc ccctttggga tacgcattat
     1261 gctcattaaa tttggcattt gatggattta ctaatgcaac ccaggattct attgcatcta
     1321 ggtatcctaa aactgaggct tgggatatta tgttaggtat gaatttgtgg ggtactattt
     1381 ataatatgat ctacatgttc ggattacctc aaggtattgg atttaaagca attcagtttt
     1441 gcaagttaca ccctgaagcc gcttgggata tattgaagta ctgcatttgt ggagctgtag
     1501 gacaaaattt tatctttatg acaatatcta attttggttc attggcaaat actacaataa
     1561 ctacaaccag aaagttcgtt tcaattgttg tatcatctgt aatgtctggt aacccattat
     1621 ctttaaaaca gtggggttgt gtatctatgg tctttggtgg tttagcttac caaatctatt
     1681 taaaatggaa gaaattacaa agagttgaag gttctgtttc taaaggtgaa gaattattca
     1741 ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg
     1801 tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta
     1861 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttatct tggggtgttc
     1921 aatgttttgc aagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc
     1981 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca
     2041 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg
     2101 attttaaaga agatggtaac attttaggtc acaaattgga atacaattat ttctctgaca
     2161 atgtttacat cactgctgac aaacaaaaga atggtatcaa agctaacttc aaaattagac
     2221 acaacattga agatggtggt gttcaattag ctgaccatta tcaacaaaat actccaattg
     2281 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct aagttatcca
     2341 aagatccaaa cgaaaagagg gaccacatgg tcttgttaga atttgttact gctgctggta
     2401 ttaccttggg tatggatgaa ttgtacaaag gatccgcaga gtatgtgcgg gccctgtttg
     2461 actttaatgg gaatgatgaa gaagaccttc cctttaagaa aggagacatc ctgagaatcc
     2521 gggataagcc tgaagagcag tggtggaatg cagaggacag cgaaggaaag agggggatga
     2581 ttcctgtccc ttacgtggag aagtatggat ctgggagcgg atctggaggc agcatggcgg
     2641 aatatgtacg agctcttttt gattttaatg gcaatgatga ggaggatctc ccttttaaaa
     2701 agggcgatat tctgcgcatt cgtgataagc cagaggaaca atggtggaac gccgaggata
     2761 gtgaggggaa acgcggcatg attccggtgc cgtatgtaga gaagtactaa tggcgccaat
     2821 tggtgcggca attgataata acgaaaatgt cttttaatga tctgggtata atgaggaatt
     2881 ttccgaacgt ttttacttta tatatatata tacatgtaac atatattcta tacgctatag
     2941 agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga gtttcacttc ttgattatcg
     3001 ctaacactaa tggttgaagt actgctactt taattttatg ctgccaaacc agatgtcaac
     3061 acagctacaa cgtatatatt aaatttgctc ttgttctgta ctttcctaat tcttatgtaa
     3121 aaagacaaga atttatgata ctatttaata acaaaaaact acctaagaaa agcatcatgc
     3181 agtcgaaatt gaaatcgaaa agtaaaactt taacggaaca tgtttgaaat tctaagaaag
     3241 catacatctt catcccttat atatagagtt atgtttgata ttagtagtca tgttgtaatc
     3301 tctggcctaa gtatacgtaa cgaaaatggt agcacgtcgc gtttatggcc cccaggttaa
     3361 tgtgttctct gaaattcgca tcactttgag aaataatggg aacaccttac gcgtgagctg
     3421 tgcccaccgc ttcgcctaat aaagcggtgt tctcaaaatt tctccccgtt ttcaggatca
     3481 cgagcgccat ctagttctgg taaaatcgcg cttacaagaa caaagaaaag aaacatcgcg
     3541 taatgcaaca gtgagacact tgccgtcata tataaggttt tggatcagta accgttattt
     3601 gagcataaca caggttttta aatatattat tatatatcat ggtatatgtg taaaattttt
     3661 ttgctgactg gttttgttta tttatttagc tttttaaaaa ttttactttc ttcttgttaa
     3721 ttttttctga ttgctctata ctcaaaccaa caacaactta ctctacaact aagatctatg
     3781 ctcatagaaa acactaatga tcggtttggt atcgtcatag atgcagggtc ttcgggttcg
     3841 agaatccatg tgtttaagtg gcaggacacg gaatcgcttc ttcatgcaac aaaccaggac
     3901 tcacagtcca tattacaatc agtacctcat attcatcaag aaaaagactg gacttttaag
     3961 ctgaatccag gcttgtcgag ttttgaaaaa aaacctcaag atgcgtacaa atctcacatc
     4021 aagccgttac tagattttgc taagaatatt atccctgaat cacattggtc gagttgtccc
     4081 gtttttattc aagcaaccgc gggcatgcgc cttttacctc aagacataca atcttccatt
     4141 ttggatggtt tgtgtcaggg gctcaaacac cctgcagaat ttttggttga ggattgctca
     4201 gcacaaattc aagtcattga tggtgaaacc gaaggtttat atggctggct tggcttaaac
     4261 tatctatatg gacactttaa tgactataat ccagaagtct ctgaccattt tacatttggg
     4321 tttatggaca tgggcggtgc ctctactcag attgcgtttg caccgcatga ttcaggagaa
     4381 atagctagac atagagatga catcgccacc atcttcttga ggagtgttaa cggagatttg
     4441 cagaaatggg acgtttttgt tagtacgtgg ttagggtttg gtgccaatca ggctagaaga
     4501 aggtacttgg ctcagttgat caataccctt ccagaaaaca caaatgatta cgaaaatgat
     4561 gacttctcta caaggaactt gaatgatcca tgtatgccgc gaggaagcag cacggatttt
     4621 gaatttaaag ataccatatt tcacatcgcg ggttctggga attacgaaca atgtacaaaa
     4681 tctatttatc cgttactttt aaagaacatg ccttgtgatg acgagccgtg cttgtttaat
     4741 ggtgttcatg ctcctcgaat agactttgcc aatgacaaat ttataggtac ttctgagtac
     4801 tggtacactg ccaacgacgt attcaaactt gggggcgaat acaactttga caaatttagc
     4861 aaaagcctaa gggagttttg caattccaat tggacgcaaa tattagcgaa cagtgataaa
     4921 ggcgtgtata attctattcc ggagaatttt ttgaaagatg catgctttaa gggcaattgg
     4981 gtccttaata tacttcatga agggtttgat atgcctcgaa tagatgtcga cgcagaaaat
     5041 gtcaatgaca ggcccttatt tcaaagtgtg gaaaaagtcg aagaacgaga gttatcgtgg
     5101 acactaggca gaattttact ttatgcctca ggtagcatat tggcaggtaa tgatgacttc
     5161 atggtgggta ttgcgcccag tgaaagaaga actaagctca ctggtaagaa attcatacct
     5221 ggcaagttac tggagtctga tcaactacgc aagcaaagtt ccggatccgg ttcattggga
     5281 agaggtagaa gatccaaatt gtaataactc gagtggcgcg aatttcttat gatttatgat
     5341 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     5401 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     5461 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     5521 cctgcaaatc gctccccatt tcgctggatg cacacactgg cttaagatga caacgccttg
     5581 ccaacaggga gttcttcaga gacatggagg ctcaaaacga aattattgac agcctagaca
     5641 tcaatagtca tacaacagaa agcgaccacc caactttggc tgataatagc gtataaacaa
     5701 tgcatacttt gtacgttcaa aatacaatgc agtagatata tttatgcata ttacatataa
     5761 tacatatcac ataggaagca acaggcgcgt tggactttta attttcgagg accgcgaatc
     5821 cttacatcac acccaatccc ccacaagtga tcccccacac accatagctt caaaatgttt
     5881 ctactccttt tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa
     5941 aacacccaag cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta
     6001 cccgtactaa aggtttggaa aagaaaaaag acaccgcctc gtttcttttt cttcgtcgaa
     6061 aaaggcaata aaaattttta tcacgtttct ttttcttgaa aatttttttt tttgattttt
     6121 ttctctttcg atgacctccc attgatattt aagttaataa acggtcatca atttctcaag
     6181 tttcagtttc atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga
     6241 aagcatagca atctaatcta agttttaatt acaaaagatc tatgccacca ccatcaagaa
     6301 gtagaataaa caaaacaaga acattaggaa tagtgggtac agctatagca gtgttggtca
     6361 cgtcctacta tatatatcaa aaggtgacag gttctgtgtc caaaggagag gagttaatca
     6421 aggaaaacat gagaatgaaa gttgtcatgg agggctccgt taatggtcac caattcaagt
     6481 gtacagggga aggtgaaggt aatccttaca tgggtacaca aactatgaga attaaagtaa
     6541 ttgaaggcgg accactacca tttgcatttg acattctggc aacgtcattc atgtacggat
     6601 cacgaacttt catcaagtac cctaaaggta taccagactt tttcaagcaa tcttttccag
     6661 agggttttac atgggaaagg gttacaagat acgaagatgg gggtgtcgtc acagttatgc
     6721 aagatacttc attagaagat ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt
     6781 ttccttctaa cggacctgtg atgcagaaaa agaccaaagg ttgggaacca aatactgaaa
     6841 tgatgtaccc agctgatgga ggtttgagag gctacacaca catggcgctt aaagttgatg
     6901 gtggaggtca tttgtcttgt agttttgtta ccacttatcg ttctaaaaag actgttggca
     6961 atatcaaaat gccaggaata catgctgtag accacagact agaaagactc gaagagagcg
     7021 ataacgaaat gttcgttgta cagagagagc atgccgtagc caaatttgct ggcttaggcg
     7081 gtggtatgga tgaattgtat aagggatccg ggtcaggctc cggatctggc cctcccccag
     7141 ctcttccgcc aaaaagacga aggtaatggc agtgctttta actaagaatt attagtcttt
     7201 tctgcttatt ttttcatcat agtttagaac actttatatt aacgaatagt ttatgaatct
     7261 atttaggttt aaaaattgat acagttttat aagttacttt ttcaaagact cgtgctgtct
     7321 attgcataat gcactggaag gggaaaaaaa aggtgcacac gcgtggcttt ttcttgaatt
     7381 tgcagtttga aaaatgctga gcagttacag agatgttacg aaccactagt gcactgcagt
     7441 acacggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
     7501 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
     7561 tgcccagtat tcttaaccca actgcacaga acaaaaacgt gcaggaaacg aagataaatc
     7621 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
     7681 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
     7741 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
     7801 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
     7861 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
     7921 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
     7981 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
     8041 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
     8101 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
     8161 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
     8221 acacccggtg tgggtttaga tgacaaggga gatgcattgg gtcaacagta tagaaccgtg
     8281 gatgatgtgg tttctacagg atctgacatt attattgttg gaagaggact atttgcaaag
     8341 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
     8401 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
     8461 tcacaaatta gagcttcaat ttaattatat cagttattac ccgagtagag cacttgaatc
     8521 cactgccccg ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa aaaaattgga
     8581 aagaaaaagc ttcatggcct ttataaaaag gaactatcca atacctcgcc agaaccaagt
     8641 aacagtattt tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca
     8701 ttgaactcca aagaacaaca agagttccaa aaagtagtgg aacaaaagca aatgaaggat
     8761 ttcatgcgtt tgtactctaa tctggtagaa agatgtttca cagactgtgt caatgacttc
     8821 acaacatcaa agctaaccaa taaggaacaa acatgcatca tgaagtgctc agaaaagttc
     8881 ttgaagcata gcgaacgtgt agggcagcgt ttccaagaac aaaacgctgc cttgggacaa
     8941 ggcttgggcc gataaggtgt actggcgtat atatatctaa ttatgtatct ctggtgtagc
     9001 ccatttttag catgtaaata taaagaccga gcggccgcgt gttacaacca attaaccaat
     9061 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     9121 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     9181 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     9241 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     9301 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     9361 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     9421 gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     9481 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     9541 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     9601 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     9661 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     9721 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     9781 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     9841 cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     9901 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     9961 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
    10021 aaggatcagt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggccg
    10801 ccaatgctaa attcgagtga aacacaggaa gatcagaaaa tcctcatttc atccatatta
    10861 acaataattt caaatgttta tttgcattat ttgaaactag gcaagacaag caacgaaacg
    10921 tttttgaaaa ttttgagtat tttcaataaa tttgtagagg actcagatat tgaaaaaaag
    10981 ctacagcaat taatacttga taagaagagt attgagaagg gcaacggttc atcatctcat
    11041 ggatctgcac atgaacaaac accagagtca aacgacgttg aaattgaggc tactgcgcca
    11101 attgatgaca atacagacga tgataacaaa ccgaagttat ctgatgtaga aaaggattaa
    11161 agatgctaag agatagtgat gatatttcat aaataatgta attctatata tgttaattac
    11221 cttttttgcg aggcatattt atggtgaagg ataagttttg accatcaaag aaggttaatg
    11281 tggctgtggt ttcagggtcc ataaagccca catggataac attacccctg aattcgcatc
    11341 tagactga
//
