LOCUS       sequence-48965-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TTK
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt cggttaaagg aagaatttat tccattttaa agcagatagg aagtggaggt
      121 tcaagcaagg tatttcaggt gttaaatgaa aagaaacaga tatatgctat aaaatatgtg
      181 aacttagaag aagcagataa ccaaactctt gatagttacc ggaacgaaat agcttatttg
      241 aataaactac aacaacacag tgataagatc atccgacttt atgattatga aatcacggac
      301 cagtacatct acatggtaat ggagtgtgga aatattgatc ttaatagttg gcttaaaaag
      361 aaaaaatcca ttgatccatg ggaacgcaag agttactgga aaaatatgtt agaggcagtt
      421 cacacaatcc atcaacatgg cattgttcac agtgatctta aaccagctaa ctttctgata
      481 gttgatggaa tgctaaagct aattgatttt gggattgcaa accaaatgca accagataca
      541 acaagtgttg ttaaagattc tcaggttggc acagttaatt atatgccacc agaagcaatc
      601 aaagatatgt cttcctccag agagaatggg aaatctaagt caaagataag ccccaaaagt
      661 gatgtttggt ccttaggatg tattttgtac tatatgactt acgggaaaac accatttcag
      721 cagataatta atcagatttc taaattacat gccataattg atcctaatca tgaaattgaa
      781 tttcccgata ttccagagaa agatcttcaa gatgtgttaa agtgttgttt aaaaagggac
      841 ccaaaacaga ggatatccat tcctgagctc ctggctcatc cctatgttca aattcaaact
      901 catccagtta accaaatggc caagggaacc actgaagaat gacagtaaag gtggata
//
