LOCUS       sequence-48966-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: KLHL12
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc agggacccag gacaagggct cgcctaggag ccaatgaagt gcttttggtg
      121 gttgggggct ttggaagcca gcagtctccc attgatgtgg tagagaaata tgaccccaag
      181 actcaggagt ggagcttttt gccaagcatc actcgtaaga gacgttatgt ggcctcagtg
      241 tcccttcatg accggatcta cgtcattggt ggctatgatg gccgttcccg ccttagttca
      301 gtggaatgtc tagactacac agcagatgag gatggggtct ggtattctgt ggcccctatg
      361 aatgtccgac gaggtcttgc tggagccacc accctgggag atatgatcta tgtctctgga
      421 ggctttgatg gaagcaggcg tcacaccagt atggagcgct atgatccaaa cattgaccag
      481 tggagcatgc tgggagatat gcagacagcc cgggaaggtg ccggactcgt agtggccagt
      541 ggagtgatct actgtctagg aggatatgac ggcttgaata tcttaaattc agttgagaaa
      601 tacgaccctc atacaggaca ttggactaat gttacaccaa tggccaccaa gcgttctggt
      661 gcaggagtag ccctgctgaa tgaccatatt tatgtggtgg ggggatttga tggtacagcc
      721 cacctttctt ccgttgaagc atacaacatt cgcactgatt cctggacaac tgtcaccagt
      781 atgaccactc cacgatgcta tgtaggggcc acagtgcttc gggggagact ctatgcaatt
      841 gcaggatatg atggtaattc cctgctaagt agcattgaat gttatgaccc tatcatcgac
      901 agctgggaag tcgtgacatc catgggaacc cagcgctgtg atgctggtgt ttgtgttctc
      961 cgcgagtgac agtaaaggtg gata
//
