LOCUS       sequence-48973-e        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GSG2
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gagaatgtag tcagaagggc cccgtaccat tctcgcactg cctccccacg
      121 gagaaattgc aacgttgtga gaaaatcggt gaaggcgtgt ttggtgaggt atttcagacc
      181 attgccgacc atactccggt cgctatcaag attatcgcga ttgaaggccc cgatctggta
      241 aacggtagtc accaaaagac atttgaggaa atcttgccag agattatcat ctccaaggaa
      301 ctgtctttgc tgtcaggcga ggtgtgcaat cgcacagaag gtttcattgg cctgaacagt
      361 gttcattgtg tccagggttc gtatcctccg ttgctgttga aagcatggga ccactacaac
      421 agcactaaag gctccgccaa tgatcgtccc gacttcttca aggatgacca actgttcatc
      481 gtgctggagt ttgagttcgg cggcattgat ttggaacaga tgcgcaccaa actctcttca
      541 ttggctacag cgaagagtat cctgcatcag ttgaccgcca gcctggcagt agctgaggcc
      601 tccctgcgtt ttgaacaccg cgacttgcat tggggtaacg tgctgttgaa gaaaacttcg
      661 ctgaagaaac tgcactatac attgaatggc aagtcgtcaa ccattccaag ttgcggtctg
      721 caagttagca tcattgatta cactttgtcc cgtctggagc gcgacggcat cgtcgtattt
      781 tgcgatgtgt cgatggacga agatttgttt accggtgacg gcgattatca gttcgacatc
      841 taccgcctca tgaagaaaga gaacaacaat cgctggggag agtatcatcc ttactctaac
      901 gttctgtggt tgcactacct gacagataag atgttgaaac aaatgacttt caagaccaaa
      961 tgcaatacac cggcgatgaa acagattaag cgcaaaatcc aagagtttca ccgcaccatg
     1021 ctgaacttct caagcgcaac tgacctgttg tgtcagcaca gtctgtttaa gtgacagtaa
     1081 aggtggata
//
