LOCUS       sequence-489747-        8492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9/sgRNA expression plasmid targeting MTF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
  TITLE     Distinct specificity and functions of PRC2 subcomplexes in human 
            stem cells and cardiac differentiation.
  JOURNAL   Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014.
  PUBMED    40803325
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014."
COMMENT     Sequence Label: pX330_MTF2_sgRNA
FEATURES             Location/Qualifiers
     source          1..8492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          192..419
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      431..440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             440..505
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             512..532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             557..4657
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             557..4657
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4658..4705
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4733..4750)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4739..4946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4955..5095
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4955..5084
                     /label=AAV2 ITR
     rep_origin      5170..5625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5257..5276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5467..5488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5642..5661)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5761..5783
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5821..5839)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5907..6011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6012..6080,6081..6872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6230..6249)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7043..7631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7532..7551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7693..7941
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7693..7713
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7864..7883
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7963..8038
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8135..8420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc gcgctcgctg
       61 cggggggggg gggggggggg aggcggagag gggcggcggc agccaatcag agcggcgcgc
      121 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
      181 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
      241 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
      301 gcccttctcc tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg
      361 ttggtggggt attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg
      421 ttggaccggt gccaccatgg actataagga ccacgacgga gactacaagg atcatgatat
      481 tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca
      541 cggagtccca gcagccgaca agaagtacag catcggcctg gacatcggca ccaactctgt
      601 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
      661 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
      721 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
      781 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
      841 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
      901 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
      961 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     1021 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     1081 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     1141 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     1201 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     1261 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     1321 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     1381 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     1441 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     1501 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     1561 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     1621 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     1681 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     1741 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     1801 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     1861 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     1921 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     1981 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     2041 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     2101 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     2161 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     2221 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     2281 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     2341 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     2401 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     2461 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     2521 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     2581 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     2641 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     2701 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     2761 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     2821 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     2881 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     2941 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     3001 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     3061 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     3121 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     3181 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     3241 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     3301 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     3361 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     3421 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     3481 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     3541 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     3601 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     3661 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     3721 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     3781 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     3841 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     3901 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
     3961 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     4021 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     4081 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     4141 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     4201 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     4261 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     4321 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     4381 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     4441 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     4501 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
     4561 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     4621 cgagacacgg atcgacctgt ctcagctggg aggcgacaaa aggccggcgg ccacgaaaaa
     4681 ggccggccag gcaaaaaaga aaaagtaaga attcctagag ctcgctgatc agcctcgact
     4741 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4801 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4861 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4921 gaagagaata gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat ggagttggcc
     4981 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     5041 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     5101 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     5161 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5221 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     5281 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5341 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     5401 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5461 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     5521 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     5581 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     5641 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5701 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5761 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5821 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5881 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5941 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6001 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6061 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6121 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6181 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6241 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6301 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6361 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6421 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6481 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6541 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6601 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6661 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6721 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6781 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6841 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6901 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6961 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7021 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7081 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7141 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7201 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7261 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7321 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7381 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7441 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7501 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7561 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7621 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7681 ttgctcacat gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag
     7741 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     7801 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     7861 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     7921 ttgtggaaag gacgaaacac cgttatcttg gcactatcaa aagttttaga gctagaaata
     7981 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     8041 ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc
     8101 gccaattctg cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc
     8161 cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga
     8221 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     8281 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     8341 ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     8401 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     8461 cccccccctc cccaccccca attttgtatt ta
//
