LOCUS       sequence-489755-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PHF19 donor plasmid with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
  TITLE     Distinct specificity and functions of PRC2 subcomplexes in human 
            stem cells and cardiac differentiation.
  JOURNAL   Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014.
  PUBMED    40803325
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014."
COMMENT     Sequence Label: pUC19_PHF19_HTC3xF_BSD_donor
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2734..2754
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             2767..3654
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             3655..3720
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6030..6151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6152..6273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6274..6395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6398..6509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6612..6645
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6646..7156
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7075..7093
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7166..7564
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7605..7829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7830..7863
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8622..8638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8622..8638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8635..8657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8646..8662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8670..8676,8677..8694,8695..8700))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8715..8736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8853..8870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9024..9612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9104..9123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9783..10574,10575..10643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10406..10425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10644..10748)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10816..10834
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctaa ttgagaacag
      421 aaggggggat tacaaaagac agatgaccca gtctacacta gctacaagcc acccagtttt
      481 cctttctagg tgaattttct ctggacagat ctcaggttag ggatggttgc aggggttggg
      541 ggtaaaaaca aaacccaact gcttgcctgg agaagatcct ccatccagag cacatggact
      601 gtgtttgctt aaggaataaa acctcagcct cctgagacag ggagtggttc aggcccgctg
      661 ggtaggggac tttgttctgt gtgggttgag agatccattc tctttaaaag gtagtgtggt
      721 atgggccatg gctctgcctt tggcccatca gcctcttctc cttgccacac ctgtttccct
      781 atctgtaaga tgtgaataat aataatgtct ccctcatagg atggtcacga ggaatcagtg
      841 agataataac caagtctggc ttatagcaca gggtacagaa atggtggttt ccttccttcc
      901 cctgatgggc tttctgaagc agggaagcaa gaagcaaaaa ccctgttctt tctctcccag
      961 tgtcagggga agctgatgga gaatcgagct ctggatccag ggactcggga ctcctatggt
     1021 gccaccagcc acctccccaa caagggggcc ctggcgaagg tcaagaacaa cttcaaagac
     1081 ttgatgtcca aactgacgga gggccagtat gtgctgtgcc ggtggacaga tggcctgtac
     1141 tacctcggga agatcaagag ggtcagcagc tctaagcaaa gctgcctcgt gactttcgaa
     1201 gataattcca aatactgggt cctatggaag gacatacagc atgccggtgt tccaggagag
     1261 gagcccaagt gcaacatctg cctagggaag acatcagggc cgctgaatga gatcctcatc
     1321 tgcgggaagt gtggcctggg ttaccaccag cagtgccaca tccccatagc gggcagtgct
     1381 gaccagcccc tgctcacacc ttggttctgc cgacgctgca tcttcgcact ggctgtgcgg
     1441 aaaggcggcg cgctgaagaa gggcgccatc gccaggacgc tgcaggccgt gaagatggtg
     1501 ctgtcctacc agcccgagga gctcgagtgg gactcgcccc atcgcaccaa ccagcagcaa
     1561 tgctactgct actgcggcgg gcccggagaa tggtacctgc ggatgctgca atgttaccgg
     1621 tgcaggcagt ggttccacga ggcctgcacc cagtgcctca atgagcccat gatgtttgga
     1681 gaccggtttt acctgttctt ctgctccgtg tgtaaccagg gcccagagta catcgagagg
     1741 ctgcccctgc gatgggtgga tgtggttcac ctggccctct ataatctggg ggtacagagc
     1801 aagaagaagt actttgactt tgaggagatt ctggcctttg tcaaccacca ctgggagctc
     1861 ctgcagcttg gcaagctcac cagcacccca gtgacagatc gaggaccaca tctcctcaac
     1921 gctctgaaca gttataaaag ccggttcctc tgcggcaagg agatcaagaa gaagaagtgc
     1981 atcttccgcc tgcgcatccg cgtcccaccc aacccgccag ggaagctgct gcctgacaaa
     2041 ggactgctgc caaatgagaa cagcgcctcc tctgagctgc gtaagagagg aaagagcaag
     2101 cctggtttgt tgcctcacga attccagcag cagaaaaggc gagtttatag aagaaaaaga
     2161 tcaaagtttt tgctggaaga tgctattccc agtagtgact tcacctcagc ctggagcacc
     2221 aaccaccacc tggctagcat atttgacttc acgctggatg aaattcaaag tttaaaaagt
     2281 gccagctcag gccagacctt cttctcagat gtcgactcca ccgacgctgc cagcacctct
     2341 ggctctgcct ccaccagcct ctcctatgac tccagatgga cagtgggcag ccgaaagagg
     2401 aagctggcag ccaaggcata catgcccctg cgggcaaagc ggtgggcagc tgagctggat
     2461 ggacgctgcc cctcggacag cagtgcagag ggggcttcag tccccgagcg gccagacgaa
     2521 ggcattgaca gccacacatt tgagagcatc agtgaagatg actcatccct gtcccacctc
     2581 aagtcatcta tcaccaacta ctttggtgca gctgggcggt tggcctgtgg ggagaagtac
     2641 caggtgttgg ctcggagggt cacacctgag ggcaaggttc agtacctggt ggagtgggaa
     2701 gggaccaccc cttacaccgg tgagccaacc actgaggatc tgtactttca gagcgataac
     2761 gcgatcgcag aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc
     2821 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac
     2881 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat
     2941 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat
     3001 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag
     3061 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat
     3121 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac
     3181 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc
     3241 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc
     3301 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc
     3361 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc
     3421 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc
     3481 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg
     3541 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg
     3601 gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgactac
     3661 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
     3721 tgaacgcgtc tagcccccgg gggtgccagg ggtcctgaaa accaaaggag gagcagcaga
     3781 agccataggc tccccagctt tctccaggct ggggtgggag aaggaagcag gacagagctg
     3841 caagtgcctg gcagaatgcc ctgcctgcct gcctgccagg ccaaggcctg cgtctctctg
     3901 ctgtaccagc tctgttccag ggcctcctca ggctcgttac ccctgtgcct gtgtctctac
     3961 acactccaca ccccctcaaa ctctgtttat ctgttctctg accttgtgtc ccctgcgctg
     4021 ggacccttcc tcctgaggcc caggtctttg tccccagttg tgtgccttga cctctctcgc
     4081 ccctttctgg gtgtgttcgc acatcctgtg tgtgcacagc tgtccctcca ctggatcccc
     4141 ttcacacgtg acccgtgggg cagccagtcc tcccagggac tacataacag gcacctttga
     4201 gagagcatgg gagaaggtgg ataagaggat gctgctcagt gcttttctct tccactttcc
     4261 tgccactccc cactaccctc ggagagaggt ggtgggatgg gagagagccc ctgtgaaagc
     4321 ctgtgaggat ctgaagagta aagggctggg tctgcctcag aaggcaccag caccagggcc
     4381 caggtattaa ggctgagagt gaaggctgcc aatgtcagct ttggaggtcc cagaagtctt
     4441 ctgttctctg gcctcacccc ctcagtcgcc atagagctgg gcctggcctt gctggaatgg
     4501 aggcatcctt ccaaacctgg gggacggggg tggggggtgg tagtggtggg agggaaacca
     4561 tgtcttgcta aacctgtttc tggtgcctcc catccccaga cccaccagac accacacagc
     4621 agacaataca cacccactcg cacaagcttc catccacatg tgttgtactt tcagctctag
     4681 gcatgcagac aaccccacac ggccacacca ccacatgccc aagtgtacac acacagagcc
     4741 acaccgtccc tctgggcctg ctggctcctc ccttggcttt cccttggccc acttccaggg
     4801 cccaggtgct gcaactaaat gtgaaagctc agtggccgct ccttctttca gcccatcaac
     4861 cagcattggt cccataggga agcacaggtg actcaccctc tttcatatcc cttgccctgc
     4921 cctgaaatgg acaatcactt tttgggatag gttgaaattt ttaaagagcc tgcatcattc
     4981 ggttccctca aagggaagcc cttgccagtg ggggtttgaa agagaatttt tggaaccaac
     5041 attcaaattc tgcctcatct ggagggaaac caaaattggg agggggaaga ggacccctga
     5101 tgttttgctg cttccagaga tattagaaac tgactcactt gattggaaaa tggacaaaag
     5161 tgccttgacg tggagggtgg gcaccagatg gggaccagcc ttgccaactg ctgctgtggc
     5221 ctccagcttg gctggttttg caggccgcca gcaggaaggc gaaggtggta gtacagcaag
     5281 aggcactggc ggggcagcag gcctgcagga gctgtttttc cattgctagg cctgacccct
     5341 ctctacctgt gagcgttcag ggggtccctg agatagttta gatgcccccc ccatcttaga
     5401 cctcagctcc cacagtgcct tttaaggggg acctcacctc ctgtgcacag cccacccact
     5461 ttcctctgct tccctggcac agcccaggca tagacgagct ggcgttggac ccagttcttc
     5521 ccccttttca gccccacagc tgctgccaca ggggccaact agggccaggt ggaaggggag
     5581 ctgagaagcc aacccctagc ccaggggtgc tgtgggaact gggatccaat ttgtagcttc
     5641 ctgcctggct tcagagagcc cagcaacctt ctaggcctgc tttccagact tctgagatag
     5701 cctgggatga gcaatcctgt tacagtacat ctggaccttc cctacctggg ctctggggag
     5761 gctgtgggcc tggagaggga aaaggaggga gggggtgtct gcaccacctg ggaagatagc
     5821 acaaggccta atgaggtcac cctgactccc caccccagca tttcattcat accagataat
     5881 agctgcatta ctgccaactg accttataac cctctgcacc ttcaaaaaga ttcatggttt
     5941 ttaattgctg cttttaataa catttgttaa agttataatt aatgtgtctg atttatgatt
     6001 taaaacctcc ctttgaacaa tcaggtacca acttgtttat tgcagcttat aatggttaca
     6061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6121 gtggtttgtc caaactcatc aatgtatctt aaacttgttt attgcagctt ataatggtta
     6181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6241 ttgtggtttg tccaaactca tcaatgtatc ttaaacttgt ttattgcagc ttataatggt
     6301 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6361 agttgtggtt tgtccaaact catcaatgta tcttagactg tgccttctag ttgccagcca
     6421 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6481 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6541 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgcg
     6601 gccgcgatat cataacttcg tataatgtat gctatacgaa gttatggggt tggggttgcg
     6661 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     6721 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     6781 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     6841 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     6901 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     6961 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     7021 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     7081 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     7141 cgacctctct ccccagcaat tcaccatggc caagcctttg tctcaagaag aatccaccct
     7201 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     7261 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     7321 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     7381 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     7441 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     7501 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     7561 ctaagctgac tcgagtctag accaattggt ttaaaccctg caggctgtgc cttctagttg
     7621 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     7681 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     7741 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7801 gcatgctggg gatgcggtgg gctctatgga taacttcgta taatgtatgc tatacgaagt
     7861 tatcctaggt ggcccctgtt ctcagactca gaatctctct gctcatgttc cagcctggct
     7921 gtcagaccag ggggcagggg gattgctgga gaattccctt cttgtccctc atgcccaaag
     7981 ctggtggtgg gaggggccag agcctggtct tgcaggcacc atccctaggg tcaacatcat
     8041 ccaactagtt ctttagaaaa gtgctctgag agcctacaaa acaaaaaagt caggtttgag
     8101 aggatcacag aggagcatct gacttgtgct tctgtcctca acattctgac caagttgcca
     8161 tccatcggtc cctggctgta cgactccagg tgactaggag tacaccctgt ccaggaactg
     8221 ccatccttct tccccttcta gccctgcttt ctggaacata cggagacaaa aatggaggag
     8281 gaagactatg ttccaggttg gcttttcccc agccctttct cacttgctcc ttcctatctg
     8341 taccccaggt cagcagctct aagcaaagct gcctcgtgac tttcgaagat aattccaaat
     8401 actgggtcct atggaaggac atacagcatg gtgagttttc ctaacatttc gggtagcccc
     8461 caacctcccc aggtcttctc tcctctgctg ccttcatttc ttccttctgt ccttccatct
     8521 cttactcctg gcatctgccc ttgaccatga accccaagcc cacctgtgat gatgatcaaa
     8581 tctggttgta tattgactta ggaaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8641 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8701 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8761 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8821 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10201 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10381 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10741 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    10801 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
