LOCUS       sequence-489789-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PHF1 donor plasmid with puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
  TITLE     Distinct specificity and functions of PRC2 subcomplexes in human 
            stem cells and cardiac differentiation.
  JOURNAL   Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014.
  PUBMED    40803325
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Aug 7:S1097-2765(25)00615-X. doi: 
            10.1016/j.molcel.2025.07.014."
COMMENT     Sequence Label: pUC19_PHF1_HTC3xF_Puro_donor
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1447..1461
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             2719..2739
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             2752..3639
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             3640..3705
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4062..4183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4184..4305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4306..4427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4430..4541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4644..4677
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4678..5188
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5107..5125
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5198..5797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5198..5217)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5694..5714
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5833..6057
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6058..6091
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6866..6882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6866..6882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6879..6901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6890..6906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6914..6920,6921..6938,6939..6944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6959..6980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7097..7114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7268..7856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7348..7367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8027..8818,8819..8887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8650..8669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8888..8992)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9060..9078
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctgg aagctgggaa
      421 actagcaact aggtttgggg tcggggggag ggctgttagt taccgagagg agagccagtc
      481 actagagttg gaagaggact caaaaagctg gtgatgggtg cccagtggtt tcctatcaag
      541 gtgtctcagg gatttggaga tgggggcaca gatacttcat tcttgatggc aaaataggca
      601 aagaaaatgg ggaggctggc cgggcccggt ggctcacgcc tgtaatccca gcactttggg
      661 aagctgaggt gggtggatca tgaggtcagg agttcgagac cagtttggcc aacatggcca
      721 acacctcatc tctactaaaa atacaaaaat tagccaggca tggtggcatg cgcctgtaat
      781 cccacctact tgggagactg aggcaggaga attgcctgaa cccgggaggt ggaggttgca
      841 gtgagccaag attgtgccac tgcactctgg cctgggcgac agagcaaaac tccatctcag
      901 gaaaaaaaaa aaaaaagaaa aagaaaatgg ggaaggtttc tcagcattca taaccttctc
      961 ctccccattt cttttctggc taggcccccc caggatgcaa tggcgcagcc cccccggctg
     1021 agccgctctg gtgcctcctc actttgggac ccagcttctc ctgctcccac ctctggcccc
     1081 aggcctcggc tttgggaggg tcaagatgtg ctggccagat ggactgatgg gctgctatac
     1141 ttgggtacca tcaaaaaggt ggacagtgct agggaggtgt gtctggtcca gtttgaggat
     1201 gattcgcagt ttctggttct atggaaagac attagccctg ctgccctccc tggagaggaa
     1261 ctcctctgtt gtgtctgtcg ctctgagact gtggtccctg ggaaccggct ggtcagctgt
     1321 gagaagtgtc gccatgctta tcaccaggac tgccatgttc ccagggctcc agcccctgga
     1381 gagggagagg gcacatcctg ggtatgccgc cagtgtgtct ttgcgatcgc caccaagagg
     1441 ggaggtgccc tgaagaaggg cccctatgcc cgggccatgc tgggtatgaa gctttctctg
     1501 ccatatggac tgaaggggct ggactgggat gctggacatc tgagcaaccg acagcagagt
     1561 tactgttact gtggtggccc tggggagtgg aacctgaaaa tgctgcagtg ccggagctgc
     1621 ctgcagtggt tccatgaggc ctgcacccag tgtctgagca agcccctcct ctatggggac
     1681 aggttctatg aatttgaatg ctgtgtgtgt cgcgggggcc ctgagaaagt ccggagacta
     1741 cagcttcgct gggtggatgt ggcccatctt gtcctgtatc acctcagtgt ttgctgtaag
     1801 aagaaatact ttgattttga tcgtgagatc ctccccttca cttctgagaa ttgggacagt
     1861 ttgctcctgg gggagctttc agacaccccc aaaggagaac gttcttccaa gctcctctct
     1921 gctcttaaca gccacaagga ccgtttcatt tcagggagag agattaagaa gaggaaatgt
     1981 ttgtttggtc tccatgctcg gatgcctccc cctgtggagc cccctactgg agatggagca
     2041 ctcaccagct tcccttcagg gcagggccct gggggagggg tctcacgtcc cctggggaag
     2101 cgccggaggc cggagccaga gcccctgagg aggaggcaga aggggaaagt ggaggagctg
     2161 gggccaccct cagcagtgcg caatcagccc gagccccagg agcagaggga gcgggctcat
     2221 ctgcagaggg cactgcaggc ctcagtgtct ccaccatccc ccagccctaa ccagagttac
     2281 cagggcagca gcggctacaa cttccggccc acagatgccc gctgcctgcc cagcagcccc
     2341 atccggatgt ttgcttcctt ccacccttct gccagcaccg cagggacctc tggggacagt
     2401 ggacccccag acaggtcacc cctggaactt cacattggtt tccccacaga catccctaaa
     2461 agtgcccccc actcgatgac tgcctcatct tcctcagttt catccccatc cccaggtctt
     2521 cctagacgct cagcaccccc ttctcccctg tgccgtagtt tgtctcctgg gactggggga
     2581 ggagtccgag gtggggttgg ttacctgtcc cgaggggacc ctgtccgggt ccttgctcgg
     2641 agagtacggc ctgatggctc tgtgcagtac ctggttgagt ggggaggagg gggcatcttc
     2701 accggtgagc caaccactga ggatctgtac tttcagagcg ataacgcgat cgcagaaatc
     2761 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
     2821 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
     2881 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
     2941 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
     3001 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     3061 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     3121 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     3181 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     3241 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     3301 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     3361 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     3421 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     3481 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     3541 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     3601 gagatcgcgc gctggctgtc gacgctcgag atttccggcg actacaaaga ccatgacggt
     3661 gattataaag atcatgacat cgattacaag gatgacgatg acaagtgaac gcgtacagcc
     3721 tgcctctgcc cagctcccca ttcacacaca ccggcacttt cataccctga cctctgacct
     3781 cacctacagc tgggatgtac ctggagagat agggggtagt tctccctact gcccaggctg
     3841 gaatccaaga gtggggagtg gggaagaggc cctcttctct accctccttc atgattcctg
     3901 acccctccca tccttcccat ttcctttgat gttattttgt tacagctttt taaatatttt
     3961 ttaaaattat ttaacccctg ggggcagaga ctgaggaggg aggatgataa gggatcccgg
     4021 actctgtatg attgaaataa agagaaataa acaaaggtac caacttgttt attgcagctt
     4081 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaacttgt ttattgcagc
     4201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaactt gtttattgca
     4321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttagac tgtgccttct
     4441 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4501 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4561 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat
     4621 agcaggcatg cggccgcgat atcataactt cgtataatgt atgctatacg aagttatggg
     4681 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     4741 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     4801 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     4861 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     4921 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     4981 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     5041 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     5101 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     5161 tgaccgaatc accgacctct ctccccagca attcaccatg accgagtaca agcccacggt
     5221 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5281 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5401 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5581 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5761 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgactc gagtctagac caattggttt
     5821 aaaccctgca ggctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5881 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5941 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6001 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggata
     6061 acttcgtata atgtatgcta tacgaagtta tcctagggta agaccttcta cctctgacct
     6121 tcttcctagt tcccttatct aattctggtt cccatttcat cctgtttgct cacccattcc
     6181 aggtggacag tgctagggag gtgtgtctgg tccagtttga ggatgattcg cagtttctgg
     6241 ttctatggaa agacattagc cctggtaaga ctctagagac ctgagattgc acatcccatg
     6301 gaaaacaaac ctggcctaga ggggcagaaa gagacttaaa ggcaggccct gtgacactgt
     6361 gttctctcac agctgccctc cctggagagg aactcctctg ttgtgtctgt cgctctgaga
     6421 ctgtggtccc tgggaaccgg ctggtcagct gtgagaagtg tcgccatggt gagagggcag
     6481 gtcacctgaa tggtccagct tgctcttccc tccaggatgg tctctatatc acctgtcctg
     6541 gccttagtcc ccaagccact gctctggcca ggctgcttag ccttatctta gtcctcatcc
     6601 gctttcagcc cagggagaag cagccatgga aaggggtggt ataacctttg caggcagaag
     6661 atggtttaaa tgcatccttt tctaaccata tgacctcgga caagtccctt aacctctagg
     6721 agcctcagtt ccctgacttg taaaataagg ataatgcacc ccctcatcaa gaccatggtc
     6781 agggattaaa tgagatgggt aaagactatt cctgtcccaa taccaagcac acacaggtat
     6841 gcaataaaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6901 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6961 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7021 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7081 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7141 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7201 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7261 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7321 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7381 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7441 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7501 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7561 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7621 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7681 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7741 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7801 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7861 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7921 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7981 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8041 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8101 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8161 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8221 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8281 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8341 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8401 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8461 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8521 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8581 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8641 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8701 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8761 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8821 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8881 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8941 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9001 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9061 ataggcgtat cacgaggccc tttcgtc
//
