LOCUS       sequence-48980-e        1521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1521)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: OXCT1
FEATURES             Location/Qualifiers
     source          1..1521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1450..1470
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1471..1488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1489..1512
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgaccaagt tttatacaga tccagtagaa gctgtaaaag acatccctga tggtgccacg
       61 gttttggttg gtggttttgg gctatgtgga attccagaga atcttataga tgctttactg
      121 aaaactggag taaaaggact aactgcagtc agcaacaatg caggggttga caattttggt
      181 ttggggcttt tgcttcggtc caagcagata aaacgcatgg tctcttcata tgtgggagaa
      241 aatgcagaat ttgaacgaca gtacttatct ggtgaattag aagtggagct gacaccacag
      301 ggcacacttg cagagaggat ccgtgcaggc ggggctggag ttcctgcatt ttacacccca
      361 acagggtatg ggaccctggt acaagaagga ggatcgccca tcaaatacaa caaagatggc
      421 agtgttgcca ttgccagtaa gccaagagag gtgagggagt tcaatggtca gcactttatt
      481 ttggaggaag caattacagg ggattttgct ttggtgaaag cctggaaggc ggaccgagca
      541 ggaaacgtga ttttcaggaa aagtgcaagg aatttcaact tgccaatgtg caaagctgca
      601 gaaaccacag tggtagaggt tgaagaaatt gtggatattg gagcatttgc tccagaagac
      661 atccatattc ctcagattta tgtacatcgc cttataaagg gagaaaaata tgagaaaaga
      721 attgagcgtt tatcaatccg gaaagaggga gatggggaag ccaaatctgc taaacctgga
      781 gatgacgtaa gggaacgaat catcaagagg gccgctcttg agtttgagga tggcatgtat
      841 gctaatttgg gcataggaat ccctctcctg gccagcaatt ttatcagccc aaatataact
      901 gttcatcttc aaagtgaaaa tggagttctg ggtttgggtc catatccacg acaacatgaa
      961 gctgatgcag atctcatcaa tgcaggcaag gaaacagtta ctattcttcc aggagcctct
     1021 tttttctcca gcgatgaatc atttgcaatg attagaggtg gacacgtcga tctgacaatg
     1081 ctaggagcga tgcaggtttc caaatatggt gacctggcta actggatgat acctgggaag
     1141 atggtgaaag gaatgggagg tgctatggat ttagtgtcca gtgcgaaaac caaagtggtg
     1201 gtcaccatgg agcattctgc aaagggaaat gcacataaaa tcatggagaa atgtacatta
     1261 ccattgactg gaaagcaatg tgtcaaccgc attattactg aaaaggctgt gtttgatgtg
     1321 gacaagaaga aagggttgac tctgattgag ctctgggaag gcctgacagt ggatgacgta
     1381 cagaagagta ctgggtgtga ttttgcagtt tcaccaaaac tcatgccaat gcagcagatc
     1441 gcaaatgcag agaacctcta cttccaatcg caccatcatc accaccatga ttacaaggat
     1501 gacgacgata agtgaggatc c
//
