LOCUS       sequence-489836-       10546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral mammalian vector with firefly luciferase gene with no 
            upstream promoter sequence; renilla luciferase and dsRed2 reporter 
            under CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar MB, Janes 
            SM, Hynds RE
  TITLE     A lentiviral toolkit to monitor airway epithelial cell 
            differentiation using bioluminescence.
  JOURNAL   Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. 
            doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.
  PUBMED    39137525
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajplung.00047.2024"; journalName: "Am J Physiol Lung Cell 
            Mol Physiol"; date: "2024-10-1- 1"; volume: "327"; issue: "4"; 
            pages: "L587-L599"
COMMENT     Sequence Label: pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2
FEATURES             Location/Qualifiers
     source          1..10546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      441..1029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     930..949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1183..1200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1317..1338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1353..1358,1359..1376,1377..1383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1391..1407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1396..1418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1415..1431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1415..1431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1450..1470
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1452..1470
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1571..1950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1951..2154
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2169..2349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2396..2521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3014..3247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3432..3476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3743..3860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3947..3956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3953..5605
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4019..4037)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     enhancer        5692..5995
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5996..6199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6228..7160
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7203..7880
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMKKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(7383..7403)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7795..7818
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    7887..8475
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7940..7960)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8358..8369)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8562..8795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8867..8988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8904..8923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8958..8977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9028..9163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9183..9202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9184..9202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9212..9229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9212..9228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9221..9243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9370..9825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9457..9476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9667..9688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9851..9955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9956..10024,10025..10546,1..270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10174..10193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
       61 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
      241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
      301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     1381 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     1441 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttggcca
     1501 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
     1561 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     1621 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1681 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1741 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1801 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1861 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1921 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     1981 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2041 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2101 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     2161 gtgaaccggg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2221 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2281 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2341 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     2401 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     2461 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     2521 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     2581 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     2641 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     2701 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     2761 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     2821 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     2881 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     2941 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     3001 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     3061 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     3121 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3181 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3241 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3301 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3361 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     3421 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     3481 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     3541 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     3601 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     3661 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     3721 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
     3781 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     3841 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagaatgga caatggatcc
     3901 agatctgcga tctaagtaag cttggcattc cggtactgtt ggtaaagcca ccatggaaga
     3961 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     4021 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     4081 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     4141 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     4201 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     4261 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     4321 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     4381 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4441 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     4501 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     4561 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     4621 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4681 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4741 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     4801 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     4861 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     4921 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     4981 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     5041 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     5101 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     5161 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     5221 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     5281 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     5341 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     5401 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5461 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5521 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5581 gaagggcgga aagatcgccg tgtaattcta gagtcgggat atcatgcagg aattcgatta
     5641 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5701 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     5761 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5821 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     5881 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5941 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     6001 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     6061 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     6121 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     6181 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcatg gcttccaagg
     6241 tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca
     6301 agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga
     6361 acgccgtgat ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc
     6421 ctcacatcga gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg
     6481 gcaagagcgg gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt
     6541 tcgagctgct gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc
     6601 tggcctttca ctactcctac gagcaccaag acaagatcaa ggccatcgtc catgctgaga
     6661 gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga catcgaggag gatatcgccc
     6721 tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa taacttcttc gtcgagacca
     6781 tgctcccaag caagatcatg cggaaactgg agcctgagga gttcgctgcc tacctggagc
     6841 cattcaagga gaagggcgag gttagacggc ctaccctctc ctggcctcgc gagatccctc
     6901 tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg caactacaac gcctaccttc
     6961 gggccagcga cgatctgcct aagatgttca tcgagtccga ccctgggttc ttttccaacg
     7021 ctattgtcga gggagctaag aagttcccta acaccgagtt cgtgaaggtg aagggcctcc
     7081 acttcagcca ggaggacgct ccagatgaaa tgggtaagta catcaagagc ttcgtggagc
     7141 gcgtgctgaa gaacgagcag ggcagcggca gcgacgaggt tgacggcagc ggcagcgaat
     7201 tcatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg
     7261 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     7321 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     7381 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
     7441 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     7501 aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct
     7561 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg aagaagaaga
     7621 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     7681 agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaaatcca
     7741 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg
     7801 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc
     7861 gccaccacct gttcctgtag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     7921 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7981 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8041 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     8101 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8161 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8221 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8281 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8341 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8401 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8461 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
     8521 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8581 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8641 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8701 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8761 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8821 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     8881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9001 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     9181 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     9241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     9301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     9361 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     9421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     9481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     9541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     9601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     9661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     9721 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     9781 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     9841 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9901 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10021 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10081 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10141 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10201 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10261 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10321 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    10381 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    10441 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    10501 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctactt
//
