LOCUS       sequence-490101-        9743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of inactive (phosphomimetic) cofilin S3E resistant to 
            shRNA in cells coexpressing mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9743)
  TITLE     Bamburg lab plasmids 2025
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: huCof S3E SSR RedTrack
FEATURES             Location/Qualifiers
     source          1..9743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..402)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(84..105)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     296..315
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   440..542
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     630..780
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    923..1044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1192..1869)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(1192..1869)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1254..1277)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1452..1471)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1721..1739
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(1912..2115)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2116..2419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    2505..2626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3375..3578)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3579..3882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   7141..7243
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7332..7349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7503..8091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7583..7602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8923..9717
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8923..9717
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8977..8996)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9587..9606
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
       61 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      121 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      181 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      241 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      301 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      361 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
      421 cgaattaatt cttaattaac atcatcaata atatacctta ttttggattg aagccaatat
      481 gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt
      541 agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt
      601 ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg
      661 ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg
      721 ggaaaactga ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat
      781 atttgtctag ggccgcgggg actttgaccg tttacgtgga gactcgccca ggtgtttttc
      841 tcaggtgttt tccgcgttcc gggtcaaagt tggcgtttta ttattatagt cagtcgagtc
      901 tagcaagcta gcttgatcgc gttaagatac attgatgagt ttggacaaac cacaactaga
      961 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1021 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     1081 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     1141 gattatgatc ttgtggtatg gctgattatg atctagagtc gcggccaatt cttaggcgcc
     1201 ggtggagtgg cggccctcgg cgcgctcgta ctgttccacg atggtgtagt cctcgttgtg
     1261 ggaggtgatg tccagcttga tgtcggtctt gtaggcgccg ggcagctgca cgggcttctt
     1321 ggccatgtag gtggtcttga cctcggcgtc gtagtggccg ccgtccttca gcttcagcct
     1381 catcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg tggaggcctc
     1441 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
     1501 cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac
     1561 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
     1621 caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt actggaactg
     1681 aggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc
     1741 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
     1801 gttcacggag ccctccatgc gcaccttgaa gcgcatgaac tccttgatga cgtcctcgga
     1861 ggaggccatc ggatcccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct
     1921 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     1981 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     2041 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     2101 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     2161 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     2221 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     2281 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     2341 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     2401 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     2461 tgattactat taatcactag cagatcgtcg ataaacctcc gcgttaagat acattgatga
     2521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2641 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2701 cctctacaaa tgtggtatgg ctgattatga tcagttatct agatccggtg gatcggatat
     2761 cttatctaga agcttggtac cgatatcctc acaaaggctt gccctccagg gagatgaccg
     2821 cactgccccc cagcttctct gccagggtgc agcggtcctt gacctcctcg tagcagtttg
     2881 cttgcaattc atgcttgatc cctgtcagct tcttcttgat ggcgtccttg gagctggcat
     2941 aaatcatttt gctcttaagg ggcgcagact cgggggccca gaagataaac accagatcct
     3001 ccttcttgct ctccttggtc tcataggttg catcatagag ggcatagcgg cagtccttat
     3061 ctggcagcat tttcacaaac gtcgcgtagg gatcgtcgac agtctggccc acatcgccca
     3121 ccaggatctc cttgccctcc tccaggatga tgttcttctt gtcctcactc aggcagaaga
     3181 gcaccgcctt cttgcgcttc ttcacctcct ctggggttga gctcttacgc accttcatgt
     3241 cgttgaacac cttgatgaca ccatcagaga cagccacacc ctcggccatg gcggtgaatt
     3301 ctgcagatat ccagcacagt ggcggccgcg tcgacggtac cagatctcta gcggatctga
     3361 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     3421 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     3481 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     3541 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3601 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3661 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3721 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3781 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3841 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     3901 ctatgaacta atgaccccgt aattgattac tattagacgt ttatcactgc tagaggatct
     3961 gggcgtggtt aagggtggga aagaatatat aaggtggggg tcttatgtag ttttgtatct
     4021 gttttgcagc agccgccgcc gccatgagca ccaactcgtt tgatggaagc attgtgagct
     4081 catatttgac aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg atgggctcca
     4141 gcattgatgg tcgccccgtc ctgcccgcaa actctactac cttgacctac gagaccgtgt
     4201 ctggaacgcc gttggagact gcagcctccg ccgccgcttc agccgctgca gccaccgccc
     4261 gcgggattgt gactgacttt gctttcctga gcccgcttgc aagcagtgca gcttcccgtt
     4321 catccgcccg cgatgacaag ttgacggctc ttttggcaca attggattct ttgacccggg
     4381 aacttaatgt cgtttctcag cagctgttgg atctgcgcca gcaggtttct gccctgaagg
     4441 cttcctcccc tcccaatgcg gtttaaaaca taaataaaaa accagactct gtttggattt
     4501 ggatcaagca agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg taggcccggg
     4561 accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg taaaggtgac
     4621 tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag caccactgca
     4681 gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag cgctgggcgt
     4741 ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc ttggtgtaag
     4801 tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga tgcatcttgg
     4861 actgtatttt taggttggct atgttcccag ccatatccct ccggggattc atgttgtgca
     4921 gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc ttagaaggaa
     4981 atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg cattcgtcca
     5041 taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg ggatcactaa
     5101 cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag cgcgggcgga
     5161 gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta ccctcacaga
     5221 tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc ggggcgatga
     5281 agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc ctgagcagct
     5341 gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccggctgc aactggtagt
     5401 taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta agcatgtccc
     5461 tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc agcgatagca
     5521 gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta ggcatgcttt
     5581 tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc tctacggcat
     5641 ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt acggcagtag
     5701 tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg tcctcgtcag
     5761 cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca gggtgcgctt
     5821 gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt cggccaggta
     5881 gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg cgcgcagctt
     5941 gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt agagcttggg
     6001 cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc agacggtctc
     6061 gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt ttcccccatg
     6121 ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct cggtgacgaa
     6181 aaggctgtcc gtgtccccgt atacagactt gagagggagt ttaaacgaat tcaatagctt
     6241 gttgcatggg cggcgatata aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc
     6301 gcaaaaaaga aagcacatcg tagtcatgct catgcagata aaggcaggta agctccggaa
     6361 ccaccacaga aaaagacacc atttttctct caaacatgtc tgcgggtttc tgcataaaca
     6421 caaaataaaa taacaaaaaa acatttaaac attagaagcc tgtcttacaa caggaaaaac
     6481 aacccttata agcataagac ggactacggc catgccggcg tgaccgtaaa aaaactggtc
     6541 accgtgatta aaaagcacca ccgacagctc ctcggtcatg tccggagtca taatgtaaga
     6601 ctcggtaaac acatcaggtt gattcacatc ggtcagtgct aaaaagcgac cgaaatagcc
     6661 cgggggaata catacccgca ggcgtagaga caacattaca gcccccatag gaggtataac
     6721 aaaattaata ggagagaaaa acacataaac acctgaaaaa ccctcctgcc taggcaaaat
     6781 agcaccctcc cgctccagaa caacatacag cgcttccaca gcggcagcca taacagtcag
     6841 ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc accagctcaa
     6901 tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac taaaaaatga
     6961 cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa
     7021 acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc cacgttacgt
     7081 cacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc gccctaaaac
     7141 ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac cccctcatta
     7201 tcatattggc ttcaatccaa aataaggtat attattgatg atgttaatta acatgcatgg
     7261 atccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     7321 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7381 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7441 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7501 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7561 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7621 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7681 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7741 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7801 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7861 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7921 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7981 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8041 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8101 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8161 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8221 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8281 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8341 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8401 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8461 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8521 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     8581 gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg
     8641 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     8701 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     8761 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     8821 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     8881 ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     8941 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     9001 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     9061 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     9121 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     9181 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     9241 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     9301 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     9361 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     9421 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     9481 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     9541 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9601 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9661 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt
     9721 ttgttaaaat ttttgttaaa tca
//
