LOCUS       sequence-490152-        6620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTwist CMV BetaGlobin encoding Siglec-7-Fc with LALA P329G hole Fc 
            and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Stark JC, Gray MA, Ibarlucea-Benitez I, Lustig M, Bond A, Cho B, 
            Govil I, Luu T, Priestley MJ, Veth TS, Errington WJ, Bruncsics B, 
            Ribi MK, Williams LA, Sarkar CA, Wisnovsky S, Riley NM, Morrissey 
            MA, Valerius T, Ravetch JV, Bertozzi CR.
  TITLE     Antibody-lectin chimeras for glyco-immune checkpoint blockade.
  JOURNAL   Nat Biotechnol 2025
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol 2025"
COMMENT     Sequence Label: TCB-Sig7FcLALAPGholeHA
FEATURES             Location/Qualifiers
     source          1..6620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1765..1791
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1844..1965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1855..1874)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1909..1928
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      2080..2126
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2156..2292
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2337..2408
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(2634..3222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2714..2733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(3968..4036,4037..4828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4186..4205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4841..4977
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5129..5200
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5205..5251)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5269..5317
                     /note="synthetic polyadenylation signal"
     enhancer        5398..5701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5703..5905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5856..5876
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6039..6514
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 ctcgagatgc tgctgctgct gctgctgccc ctgctctggg ggagggagag ggtggaagga
       61 cagaagagta accggaagga ttactcgctg acgatgcaga gttccgtgac cgtgcaagag
      121 ggcatgtgtg tccatgtgcg ctgctccttc tcctacccag tggacagcca gactgactct
      181 gacccagttc atggctactg gttccgggca gggaatgata taagctggaa ggctccagtg
      241 gccacaaaca acccagcttg ggcagtgcag gaggaaactc gggaccgatt ccacctcctt
      301 ggggacccac agaccaaaaa ttgcaccctg agcatcagag atgccagaat gagtgatgcg
      361 gggagatact tctttcgtat ggagaaagga aatataaaat ggaattataa atatgaccag
      421 ctctctgtga acgtgacagc cttgacccac aggcccaaca tccttatccc cggtaccctg
      481 gagtctggct gcttccagaa tctgacctgc tctgtgccct gggcctgtga gcaggggacg
      541 ccccctatga tctcctggat ggggacctct gtgtcccccc tgcacccctc caccacccgc
      601 tcctcagtgc tcaccctcat cccacagccc cagcaccacg gcaccagcct cacctgtcag
      661 gtgaccttgc ctggggccgg cgtgaccacg aacaggacca tccaactcaa tgtgtcctac
      721 cctcctcaga acttgactgt gactgtcttc caaggagaag gcacagcatc cacagctctg
      781 gggaacagct catctctttc agtcctagag ggccagtctc tgcgcttggt ctgtgctgtt
      841 gacagcaatc cccctgccag gctgagctgg acctggagga gtctgaccct gtacccctca
      901 cagccctcaa accctctggt actggagctg caagtgcacc tgggggatga aggggaattc
      961 acctgtcgag ctcagaactc tctgggttcc cagcacgttt ccctgaacct ctccctgcaa
     1021 caggagtaca caggcaaaat gaggcctgta tcaggaggcg gaggtggagg ccccaaatct
     1081 tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagcagccgg gggaccgtca
     1141 gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc
     1201 acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg
     1261 gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg
     1321 taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac
     1381 aagtgcaagg tctccaacaa agccctcgga gcccccatcg agaaaaccat ctccaaagcc
     1441 aaagggcagc cccgagaacc acaggtgtgc accctgcccc catcccggga tgagctgacc
     1501 aagaaccagg tcagcctgag ctgcgcggtc aaaggcttct atcccagcga catcgccgtg
     1561 gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac
     1621 tccgacggct ccttcttcct cgtgagcaag ctcaccgtgg acaagagcag gtggcagcag
     1681 gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag
     1741 agcctctccc tgtctccggg tgggtaccca tacgatgttc cagattacgc ttgagctagc
     1801 ttgactgact gagatacagc gtaccttcag ctcacagaca tgataagata cattgatgag
     1861 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1921 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1981 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2041 ctctacaaat gtggtattgg cccatctcta tcggtatcgt agcataaccc cttggggcct
     2101 ctaaacgggt cttgaggggt tttttgtgcc cctcgggccg gattgctatc taccggcatt
     2161 ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat
     2221 tcagcaacgg atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa
     2281 atttatcctt aaggtcgtca gctatcctgc aggcgatctc tcgatttcga tcaagacatt
     2341 cctttaatgg tcttttctgg acaccactag gggtcagaag tagttcatca aactttcttc
     2401 cctccctaat ctcattggtt accttgggct atcgaaactt aattaaccag tcaagtcagc
     2461 tacttggcga gatcgacttg tctgggtttc gactacgctc agaattgcgt cagtcaagtt
     2521 cgatctggtc cttgctattg cacccgttct ccgattacga gtttcattta aatcatgtga
     2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3421 atctcagcga tctgtctatt tcgttcatcc atagttgcat ttaaatttcc gaactctcca
     3481 aggccctcgt cggaaaatct tcaaaccttt cgtccgatcc atcttgcagg ctacctctcg
     3541 aacgaactat cgcaagtctc ttggccggcc ttgcgccttg gctattgctt ggcagcgcct
     3601 atcgccaggt attactccaa tcccgaatat ccgagatcgg gatcacccga gagaagttca
     3661 acctacatcc tcaatcccga tctatccgag atccgaggaa tatcgaaatc ggggcgcgcc
     3721 tggtgtaccg agaacgatcc tctcagtgcg agtctcgacg atccatatcg ttgcttggca
     3781 gtcagccagt cggaatccag cttgggaccc aggaagtcca atcgtcagat attgtactca
     3841 agcctggtca cggcagcgta ccgatctgtt taaacctaga tattgatagt ctgatcggtc
     3901 aacgtataat cgagtcctag cttttgcaaa catctatcaa gagacaggat cagcaggagg
     3961 ctttcgcatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4021 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4081 gggtgcgcga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4141 tcgccccgaa gaacgctttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4201 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4261 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4321 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4381 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4441 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4501 cacgatgcct gtagcaatgg caacaacctt gcgtaaacta ttaactggcg aactacttac
     4561 tctagcttcc cggcaacagt tgatagactg gatggaggcg gataaagttg caggaccact
     4621 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4681 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4741 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4801 aggtgcctca ctgattaagc attggtaacc gattctaggt gcattggcgc agaaaaaaat
     4861 gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg
     4921 gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta tccttaagat
     4981 cccgaatcgt ttaaactcga ctctggctct atcgaatctc cgtcgtttcg agcttacgcg
     5041 aacagccgtg gcgctcattt gctcgtcggg catcgaatct cgtcagctat cgtcagctta
     5101 cctttttggc agcgatcgcg gctcccgaca tcttggacca ttagctccac aggtatcttc
     5161 ttccctctag tggtcataac agcagcttca gctacctctc aattcaaaaa acccctcaag
     5221 acccgtttag aggccccaag gggttatgct atcaatcgtt gcgttacaca cacaaaaaac
     5281 caacacacat ccatcttcga tggatagcga ttttattatc taactgctga tcgagtgtag
     5341 ccagatctag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     5401 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     5461 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     5521 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     5581 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     5641 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     5701 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     5761 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     5821 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     5881 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcgcct ggagacgcca
     5941 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct
     6001 tacatgtggt accgagctcg gatcctgaga acttcagggt gagtctatgg gacccttgat
     6061 gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca
     6121 gggtacacat attgaccaaa tcagggtaat tttgcatttg taattttaaa aaatgctttc
     6181 ttcttttaat atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt
     6241 cagggcaata atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga
     6301 taatttctgg gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt
     6361 gtaactgatg taagaggttt catattgcta atagcagcta caatccagct accattctgc
     6421 ttttatttta tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct
     6481 aatcatgttc atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt
     6541 gctggcccat cactttggca aaggcggccg ctgccaagct tccgagctct cgaattcaaa
     6601 ggaggtaccg cgatatctac
//
