LOCUS       sequence-490160-        1156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for activated ARIH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1156)
  TITLE     E2 Variants for Probing E3 Ubiquitin Ligase Activities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GST-tag ARIH2 binding E2 variant L3A2-1
FEATURES             Location/Qualifiers
     source          1..1156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(570..592)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(737..755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        823..927
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggttgcg gccagcagca
       61 ggctgatgaa ggagcttgaa gaaatccgca aagcagggat gaaaaacttc cgtaacatcc
      121 aggttgatga agctaattta ttgacttggc aagggcttat tgttcctgac aaccctccat
      181 atgataaggg agccttcaga atcgaaatca actttccagc agagtaccca ttcaaaccac
      241 cgaagatcac atttaaaaca aagatctatc acccaaacat cgacgaaaag gggcaggtct
      301 gtctgccagt aattagtgcc gaaaactgga agccagcaac caaaaccgac caagtaatcc
      361 agtccctcat agcactggtg aatgaccccc agcctgagca cccgcttcgg gctgacctag
      421 ctgaagaata ctctaaggac cgtaaaaaat tcgcaaagaa tgctgaagag tttacaaaga
      481 aatatgggga aaagcgacct gtggactaag cggccgcatc gtgactgact gacgatctgc
      541 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
      601 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      661 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat
      721 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      781 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cmcttttaaa
     1141 gttctgctwt gtggcg
//
