LOCUS       sequence-490163-       10940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral-SREBP-1c cleavage-activation reporter system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Pena de la Sancha P, Wieser BI, Schauer S, Reicher H, Sattler W, 
            Breinbauer R, Schweiger M, Lechleitner M, Frank S, Zechner R, Kratky
            D, Espenshade PJ, Hoefler G, Vesely PW
  TITLE     Lipolysis-derived fatty acids are needed for homeostatic control of 
            sterol element-binding protein-1c driven hepatic lipogenesis.
  JOURNAL   Commun Biol. 2025 Apr 9;8(1):588. doi: 10.1038/s42003-025-08002-1.
  PUBMED    40205023
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-04-9"; volume: "8(1):588. doi"; pages: " 
            10.1038/s42003-025-08002-1"
COMMENT     Sequence Label: pShuttle-CMV-Flag-SREBP-1c
FEATURES             Location/Qualifiers
     source          1..10940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        399..702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        703..906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     857..877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      978..987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             987..1052
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4587..4708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4624..4643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4678..4697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7899..8001
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(8090..8107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8261..8849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8341..8360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9681..10475
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9681..10475
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9735..9754)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10345..10364
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(10523..10903)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10585..10606)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10797..10816
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgtcga cgcggccgca
      961 ccggtaggcc tcgtacggcc accatggatt acaaggatca cgacggagat tacaaggatc
     1021 acgacatcga ttacaaggat gacgacgata agggatccat ggattgcact ttcgaagaca
     1081 tgcttcagct tatcaacaac caagacagtg acttccctgg cctatttgac ccaccctatg
     1141 ctgggagtgg ggcagggggc acagaccctg ccagccccga taccagctcc ccaggcagct
     1201 tgtctccacc tcctgccaca ttgagctcct ctcttgaagc cttcctgagc gggccgcagg
     1261 cagcgccctc acccctgtcc cctccccagc ctgcacccac tccattgaag atgtacccgt
     1321 ccatgcccgc tttctcccct gggcctggta tcaaggaaga gtcagtgcca ctgagcatcc
     1381 tgcagacccc caccccacag cccctgccag gggccctcct gccacagagc ttcccagccc
     1441 cagccccacc gcagttcagc tccacccctg tgttaggcta ccccagccct ccgggaggct
     1501 tctctacagg aagccctccc gggaacaccc agcagccgct gcctggcctg ccactggctt
     1561 ccccgccagg ggtcccgccc gtctccttgc acacccaggt ccagagtgtg gtcccccagc
     1621 agctactgac agtcacagct gcccccacgg cagcccctgt aacgaccact gtgacctcgc
     1681 agatccagca ggtcccggtc ctgctgcagc cccacttcat caaggcagac tcgctgcttc
     1741 tgacagccat gaagacagac ggagccactg tgaaggcggc aggtctcagt cccctggtct
     1801 ctggcaccac tgtgcagaca gggcctttgc cgaccctggt gagtggcgga accatcttgg
     1861 caacagtccc actggtcgta gatgcggaga agctgcctat caaccggctc gcagctggca
     1921 gcaaggcccc ggcctctgcc cagagccgtg gagagaagcg cacagcccac aacgccattg
     1981 agaagcgcta ccgctcctcc atcaatgaca aaatcattga gctcaaggat ctggtggtgg
     2041 gcactgaggc aaagctgaat aaatctgctg tcttgcgcaa ggccatcgac tacattcgct
     2101 ttctgcaaca cagcaaccag aaactcaagc aggagaacct aagtctgcgc actgctgtcc
     2161 acaaaagcaa atctctgaag gatctggtgt cggcctgtgg cagtggaggg aacacagacg
     2221 tgctcatgga gggcgtgaag actgaggtgg aggacacact gaccccaccc ccctcggatg
     2281 ctggctcacc tttccagagc agccccttgt cccttggcag caggggcagt ggcagcggtg
     2341 gcagtggcag tgactcggag cctgacagcc cagtctttga ggacagcaag gcaaagccag
     2401 agcagcggcc gtctctgcac agccggggca tgctggaccg ctcccgcctg gccctgtgca
     2461 cgctcgtctt cctctgcctg tcctgcaacc ccttggcctc cttgctgggg gcccgggggc
     2521 ttcccagccc ctcagatacc accagcgtct accatagccc tgggcgcaac gtgctgggca
     2581 ccgagagcag agatggccct ggctgggccc agtggctgct gcccccagtg gtctggctgc
     2641 tcaatgggct gttggtgctc gtctccttgg tgcttctctt tgtctacggt gagccagtca
     2701 cacggcccca ctcaggcccc gccgtgtact tctggaggca tcgcaagcag gctgacctgg
     2761 acctggcccg gggagacttt gcccaggctg cccagcagct gtggctggcc ctgcgggcac
     2821 tgggccggcc cctgcccacc tcccacctgg acctggcttg tagcctcctc tggaacctca
     2881 ttcgtcacct gctgcagcgt ctctgggtgg gccgctggct ggcaggccgg gcagggggcc
     2941 tgcagcagga ctgtgctctg cgagtggatg ctagcgccag cgcccgagac gcagccctgg
     3001 tctaccataa gctgcaccag ctgcacacca tggggaagca cacaggcggg cacctcactg
     3061 ccaccaacct ggcgctgagt gccctgaacc tggcagagtg tgcaggggat gccgtgtctg
     3121 tggcgacgct ggccgagatc tatgtggcgg ctgcattgag agtgaagacc agtctcccac
     3181 gggccttgca ttttctgaca cgcttcttcc tgagcagtgc ccgccaggcc tgcctggcac
     3241 agagtggctc agtgcctcct gccatgcagt ggctctgcca ccccgtgggc caccgtttct
     3301 tcgtggatgg ggactggtcc gtgctcagta ccccatggga gagcctgtac agcttggccg
     3361 ggaacccagt ggaccccctg gcccaggtga ctcagctatt ccgggaacat ctcttagagc
     3421 gagcactgaa ctgtgtgacc cagcccaacc ccagccctgg gtcagctgat ggggacaagg
     3481 agttctcgga tgccctcggg tacctgcagc tgctgaacag ctgttctgat gctgcggggg
     3541 ctcctgccta cagcttctcc atcagttcca gcatggccac caccaccggc gtagacccgg
     3601 tggccaagtg gtgggcctct ctgacagctg tggtgatcca ctggctgcgg cgggatgagg
     3661 aggcggctga gcggctgtgc ccgctggtgg agcacctgcc ccgggtgctg caggagtctg
     3721 agagacccct gcccagggca gctctgcact ccttcaaggc tgcccgggcc ctgctgggct
     3781 gtgccaaggc agagtctggt ccagccagcc tgaccatctg tgagaaggcc agtgggtacc
     3841 tgcaggacag cctggctacc acaccagcca gcagctccat tgacaaggcc gtgcagctgt
     3901 tcctgtgtga cctgcttctt gtggtgcgca ccagcctgtg gcggcagcag cagcccccgg
     3961 ccccggcccc agcagcccag ggcaccagca gcaggcccca ggcttccgcc cttgagctgc
     4021 gtggcttcca acgggacctg agcagcctga ggcggctggc acagagcttc cggcccgcca
     4081 tgcggagggt gttcctacat gaggccacgg cccggctgat ggcgggggcc agccccacac
     4141 ggacacacca gctcctcgac cgcagtctga ggcggcgggc aggccccggt ggcaaaggag
     4201 gcgcggtggc ggagctggag ccgcggccca cgcggcggga gcacgcggag gccttgctgc
     4261 tggcctcctg ctacctgccc cccggcttcc tgtcggcgcc cgggcagcgc gtgggcatgc
     4321 tggctgaggc ggcgcgcaca ctcgagaagc ttggcgatcg ccggctgctg cacgactgtc
     4381 agcagatgct catgcgcctg ggcggtggga ccactgtcac ttccagctag gaattccgcc
     4441 ccccccccct aacgatccga tccaccggat ctagataact gatcataatc agccatacca
     4501 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4561 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4681 gtttgtccaa actcatcaat gtatcttaac gcggatctgg gcgtggttaa gggtgggaaa
     4741 gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc
     4801 catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa cgcgcatgcc
     4861 cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc gccccgtcct
     4921 gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt tggagactgc
     4981 agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga ctgactttgc
     5041 tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt
     5101 gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg tttctcagca
     5161 gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc ccaatgcggt
     5221 ttaaaacata aataaaaaac cagactctgt ttggatttgg atcaagcaag tgtcttgctg
     5281 tctttattta ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag
     5341 ggtcctgtgt attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg
     5401 cataagcccg tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt
     5461 gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag
     5521 tagcaagctg attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg
     5581 ggatgggtgc atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat
     5641 gttcccagcc atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc
     5701 ggtgcacttg ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac
     5761 gcccttgtga cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg
     5821 ggcggcggcc tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat
     5881 gagatcgtca taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat
     5941 ggttccatcc ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag
     6001 ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg
     6061 ggagatcagc tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg
     6121 cccgtaaatc acacctatta ccggctgcaa ctggtagtta agagagctgc agctgccgtc
     6181 atccctgagc aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac
     6241 caaatccgcc agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt
     6301 tttcaacggt ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc
     6361 caggcggtcc cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg
     6421 tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc
     6481 agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag
     6541 gggtgcgctc cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg
     6601 aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag
     6661 tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac
     6721 gaggggcagt gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg
     6781 gagtaggcat ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc
     6841 tctggccgtt cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct
     6901 ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat
     6961 acagacttga gagggagttt aaacgaattc aatagcttgt tgcatgggcg gcgatataaa
     7021 atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta
     7081 gtcatgctca tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat
     7141 ttttctctca aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac
     7201 atttaaacat tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg
     7261 actacggcca tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc
     7321 gacagctcct cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga
     7381 ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg
     7441 cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac
     7501 acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca
     7561 acatacagcg cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac
     7621 ctattaaaaa aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg
     7681 ccaagtgcag agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa
     7741 aaacacccag aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa
     7801 cttcctcaaa tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact
     7861 acaattccca acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc
     7921 acgccccgcg ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa
     7981 taaggtatat tattgatgat gttaattaac atgcatggat ccatatgcgg tgtgaaatac
     8041 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     8101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8701 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9301 cagttaatag tttgcgcaac gttgttgcca ttgctgcagc catgagatta tcaaaaagga
     9361 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     9421 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     9481 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     9541 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     9601 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     9661 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     9721 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     9781 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     9841 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     9901 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     9961 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    10021 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    10081 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    10141 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    10201 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    10261 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    10321 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    10381 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    10441 tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc
    10501 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    10561 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    10621 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    10681 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    10741 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    10801 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    10861 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga
    10921 tcgaattaat tcttaattaa
//
