LOCUS       sequence-490174-        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for activated ARIH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  TITLE     E2 Variants for Probing E3 Ubiquitin Ligase Activities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GST-tag ARIH2 binding E2 variant L3A2-3
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(572..594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(739..757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        825..929
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccatcctcca aaatcggatg aaaacctgta ttttcaggga tccatgtatg cggcccatgg
       61 gaggctgcct cgggagcttg aagaaatccg caaagcaggg atgaaaaact tccgtaacat
      121 ccaggttgat gaagctaatt tattgacttg gcaagggctt attgttcctg acaaccctcc
      181 atatgataag ggagccttca gaatcgaaat caactttcca gcagagtacc cattcaaacc
      241 accgaagatc acatttaaaa caaagatcta tcacccaaac atcgacgaaa aggggcaggt
      301 ctgtctgcca gtaattagtg ccgaaaactg gaagccagca accaaaaccg accaagtaat
      361 ccagtccctc atagcactgg tgaatgaccc ccagcctgag cacccgcttc gggctgacct
      421 agctgaagaa tactctaagg accgtaaaaa attcgcaaag aatgctgaag agtttacaaa
      481 gaaatatggg gaaaagcgac ctgtggacta agcggccgca tcgtgactga ctgacgatct
      541 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      601 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      661 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      721 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      781 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1021 taaagatgct gaagatcagt ggtgscacga gtgggttaca tcgaactgga tctcaacagc
     1081 ggtaagatcc ttgagagttt cgccccgaag aacgttttcc atgatgagca cttttaargt
//
