LOCUS       sequence-490184-        1228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for LUBAC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1228)
  TITLE     E2 Variants for Probing E3 Ubiquitin Ligase Activities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GST-tag LUBAC binding E2 variant D3LU-2
FEATURES             Location/Qualifiers
     source          1..1228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(551..573)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(718..736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        804..908
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1127..1146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccatcctcca aaatcggatg aaaacctgta ttttcaggga tccatggcac tgaaacgcat
       61 taacaaagaa ctgagcgatc tggcacgtga tccgcctgca cagattagcg caggtccggt
      121 tggtgatgat atgtttcatt ggcaggcaac cattatgggt ccgaatgata gcccgtatca
      181 aggtggtgtt ttttttctga ccattcactt tccgaccgac tatccgttta aaccgcctaa
      241 agttgcattt accacgcgta tttatcatcc gaacattaat agcaatggca gcatttgtct
      301 ggatattctg cgtagccagt ggatggcggc actgaccatt agcaaagttc tgctgagcat
      361 tgcaagcctg ctggatgatc cgaatccgga tgatcctctg gttccggaaa ttgcacgtat
      421 ctataaaacc gatcgcgata aatacaatcg cattagccgt gaatggaccc agaaatatgc
      481 aatgtaatga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg
      541 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      601 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      661 cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc
      721 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1141 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacmc tattctcaga
     1201 atgacttggt tgagtactca ccagtcmc
//
