LOCUS       sequence-490223-        4810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2301m is an enhancer sequence designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4810)
  TITLE     Basal Ganglia/CERP Enhancer AAVs
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2067 - pAAV-AiE2301m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1120..1231
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1349..1489
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1349..1478
                     /label=AAV2 ITR
     rep_origin      1564..2019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1651..1670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1861..1882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2036..2055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2155..2177
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2215..2233)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2301..2405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2406..2474,2475..3266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2624..2643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3437..4025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3926..3945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4087..4216
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gatccagatc tttcgaagct agccaccatg gtgcccaaga agaagaggaa agtctccaac
       61 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc
      121 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag
      181 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg
      241 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg
      301 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc
      361 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag
      421 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac
      481 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc
      541 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag
      601 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg
      661 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga
      721 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga
      781 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg
      841 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac
      901 ctggcctggt ctggccactc tgccagagtg ggtgctgcca ccgacatggc cagggctggt
      961 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat tgtgatgaac
     1021 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctcga agatggggac
     1081 tgaggcgcgc cgaattcaag cttctcgaga gatcttcgac tgtgccttct agttgccagc
     1141 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1201 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1261 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1321 agatctcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     1381 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     1441 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     1501 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     1561 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1621 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1741 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     1801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1861 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     1921 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     1981 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     2041 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     2101 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2161 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     2221 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2281 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2341 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2401 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2461 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2521 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2581 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2641 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2701 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2761 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2821 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2881 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2941 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3001 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3061 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3121 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3181 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3241 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3301 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4081 acatgtcctg caggcagctg cgcgctcgct cgctcactga ggccgcccgg gcgtcgggcg
     4141 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
     4201 atcactaggg gttcctgcgg ccgcacgcgt tgtaagagta ataatcgaga gagaaaatag
     4261 tatctctttc aactttaact tatcaataaa aaaaattcta tgttttttca gttagaaaca
     4321 caaacttatt ttcaaaagca acactataaa ccatgctggc aacagctagc acactcagaa
     4381 gagccaaagg tcttattatc cttcctatgc aaggccaatt ttaatgaaat atacttggca
     4441 gtgtatttct ctttttaaag acaagcaata gtttgaaccc cttagataat ggcaatgacc
     4501 aagtcaggtg tgcagtatgc ccaggacaga ctttattaga gttctgttat aaacacacag
     4561 tctcttgaca ctaaagatgt agttcttgca gataaggctg ggaggcagga aatgaatctc
     4621 ctcccaaatg ctgcttctta tctttaaagt aacctcaaat tattactaaa ctaataaagt
     4681 aattttgttt cagaaagtga tctgatctat tttattttga gctctcgata agtcgaagag
     4741 tgagatgtcg gagctcgggc tgggcataaa agtcagggca gagccatcta ttgcttacat
     4801 ttgcttctgg
//
