LOCUS       sequence-49028-e        1204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1204)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HAO1
FEATURES             Location/Qualifiers
     source          1..1204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tcccccggct aatttgtatc aatgattatg aacaacatgc taaatcagta
      121 cttccaaagt ctatatatga ctattacagg tctggggcaa atgatgaaga aactttggct
      181 gataatattg cagcattttc cagatggaag ctgtatccaa ggatgctccg gaatgttgct
      241 gaaacagatc tgtcgacttc tgttttagga cagagggtca gcatgccaat atgtgtgggg
      301 gctacggcca tgcagcgcat ggctcatgtg gacggcgagc ttgccactgt gagagcctgt
      361 cagtccctgg gaacgggcat gatgttgagt tcctgggcca cctcctcaat tgaagaagtg
      421 gcggaagctg gtcctgaggc acttcgttgg ctgcaactgt atatctacaa ggaccgagaa
      481 gtcaccaaga agctagtgcg gcaggcagag aagatgggct acaaggccat atttgtgaca
      541 gtggacacac cttacctggg caaccgtctg gatgatgtgc gtaacagatt caaactgccg
      601 ccacaactca ggatgaaaaa ttttgaaacc agtactttat cattttctcc tgaggaaaat
      661 tttggagacg acagtggact tgctgcatat gtggctaaag caatagaccc atctatcagc
      721 tgggaagata tcaaatggct gagaagactg acatcattgc caattgttgc aaagggcatt
      781 ttgagaggtg atgatgccag ggaggctgtt aaacatggct tgaatgggat cttggtgtcg
      841 aatcatgggg ctcgacaact cgatggggtg ccagccacta ttgatgttct gccagaaatt
      901 gtggaggctg tggaagggaa ggtggaagtc ttcctggacg ggggtgtgcg gaaaggcact
      961 gatgttctga aagctctggc tcttggcgcc aaggctgtgt ttgtggggag accaatcgtt
     1021 tggggcttag ctttccaggg ggagaaaggt gttcaagatg tcctcgagat actaaaggaa
     1081 gaattccggt tggccatggc tctgagtggg tgccagaatg tgaaagtcat cgacaagaca
     1141 ttggtgagga aaaatccttt ggccgtttcc aagatctgac agtaaaggtg gatacggatc
     1201 cgaa
//
