LOCUS       sequence-490309-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for protein expression of 2x StrepII-tagged Arabidopsis 
            heterotrimeric G protein GPA1-Q222L (""constitutively active"" 
            mutant) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Gookin TE, Chakravorty D, Assmann SM
  TITLE     Influence of expression and purification protocols on Galpha 
            biochemical activity: kinetics of plant and mammalian G protein 
            cycles.
  JOURNAL   Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 
            10.3389/fmolb.2025.1513660. eCollection 2025.
  PUBMED    40260404
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Biosci."; date: "2025-04-7"; volume: "12:1513660. doi"; pages: " 
            10.3389/fmolb.2025.1513660. eCollection 2025"
COMMENT     Sequence Label: pSTTa-GPA1-Q222L
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggcttac tctgcagtag aagtcgacat catactgaag atactgatga gaatacacag
       61 gctgctgaaa tcgaaagacg gatagagcaa gaagcaaagg ctgaaaagca tattcggaag
      121 cttttgctac ttggtgctgg ggaatctgga aaatctacaa tttttaagca gataaaactt
      181 ctattccaaa cgggatttga tgaaggagaa ctaaagagct atgttccagt cattcatgcc
      241 aatgtctatc agactataaa attattgcat gatggaacaa aggagtttgc tcaaaatgaa
      301 acagattctg ctaaatatat gttatcttct gaaagtattg caattgggga gaaactatct
      361 gagattggtg gtaggttaga ctatccacgt cttaccaagg acatcgctga gggaatagaa
      421 acactatgga aggatcctgc aatccaggaa acttgtgctc gtggtaatga gcttcaggtt
      481 cctgattgta cgaaatatct gatggagaac ttgaagagac tatcagatat aaattatatt
      541 ccaactaagg aggatgtact ttatgcaaga gttcgcacaa ctggtgtcgt ggaaatacag
      601 ttcagccctg tgggagagaa taaaaaaagt ggtgaagtgt accgattgtt tgacgtgggt
      661 ggactgagaa atgagaggag gaaatggatt catctgtttg aaggtgtaac agctgtgata
      721 ttttgtgctg ccatcagcga gtacgaccaa acgctctttg aggacgagca gaaaaacagg
      781 atgatggaga ccaaggaatt attcgactgg gtcctgaaac aaccctgttt tgagaaaaca
      841 tccttcatgc tgttcttgaa caagttcgac atatttgaga agaaagttct tgacgttccg
      901 ttgaacgttt gcgagtggtt cagagattac caaccagttt caagtgggaa acaagagatt
      961 gagcatgcat acgagtttgt gaagaagaag tttgaggagt tatattacca gaacacggcg
     1021 ccggatagag tggacagggt attcaaaatc tacaggacga cggctttgga ccagaagctt
     1081 gtaaagaaaa cgttcaagct cgtagatgag acactaagaa ggagaaattt actggaggct
     1141 ggccttttat ga
//
