LOCUS       sequence-490311-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for protein expression of 2x StrepII-tagged human 
            heterotrimeric G protein GNAI1 in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Gookin TE, Chakravorty D, Assmann SM
  TITLE     Influence of expression and purification protocols on Galpha 
            biochemical activity: kinetics of plant and mammalian G protein 
            cycles.
  JOURNAL   Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 
            10.3389/fmolb.2025.1513660. eCollection 2025.
  PUBMED    40260404
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Biosci."; date: "2025-04-7"; volume: "12:1513660. doi"; pages: " 
            10.3389/fmolb.2025.1513660. eCollection 2025"
COMMENT     Sequence Label: pSTTa-GNAI1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggctgca cgctgagcgc cgaggacaag gcggcggtgg agcggagtaa gatgatcgac
       61 cgcaacctcc gtgaggacgg cgagaaggcg gcgcgcgagg tcaagctgct gctgctcggt
      121 gctggtgaat ctggtaaaag tacaattgtg aagcagatga aaattatcca tgaagctggt
      181 tattcagaag aggagtgtaa acaatacaaa gcagtggtct acagtaacac catccagtca
      241 attattgcta tcattagggc tatggggagg ttgaagatag actttggtga ctcagcccgg
      301 gcggatgatg cacgccaact ctttgtgcta gctggagctg ctgaagaagg ctttatgact
      361 gcagaacttg ctggagttat aaagagattg tggaaagata gtggtgtaca agcctgtttc
      421 aacagatccc gagagtacca gcttaatgat tctgcagcat actatttgaa tgacttggac
      481 agaatagctc aaccaaatta catcccgact caacaagatg ttctcagaac tagagtgaaa
      541 actacaggaa ttgttgaaac ccattttact ttcaaagatc ttcattttaa aatgtttgat
      601 gtgggaggtc agagatctga gcggaagaag tggattcatt gcttcgaagg agtgacggcg
      661 atcatcttct gtgtagcact gagtgactac gacctggttc tagctgaaga tgaagaaatg
      721 aaccgaatgc atgaaagcat gaaattgttt gacagcatat gtaacaacaa gtggtttaca
      781 gatacatcca ttatactttt tctaaacaag aaggatctct ttgaagaaaa aatcaaaaag
      841 agccctctca ctatatgcta tccagaatat gcaggatcaa acacatatga agaggcagct
      901 gcatatattc aatgtcagtt tgaagacctc aataaaagaa aggacacaaa ggaaatatac
      961 acccacttca catgtgccac agatactaag aatgtgcagt ttgtttttga tgctgtaaca
     1021 gatgtcatca taaaaaataa tctaaaagat tgtggtctct tttaa
//
