LOCUS       sequence-490313-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for protein expression of 2x StrepII-tagged human 
            heterotrimeric G protein GNAO1 in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Gookin TE, Chakravorty D, Assmann SM
  TITLE     Influence of expression and purification protocols on Galpha 
            biochemical activity: kinetics of plant and mammalian G protein 
            cycles.
  JOURNAL   Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 
            10.3389/fmolb.2025.1513660. eCollection 2025.
  PUBMED    40260404
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Biosci."; date: "2025-04-7"; volume: "12:1513660. doi"; pages: " 
            10.3389/fmolb.2025.1513660. eCollection 2025"
COMMENT     Sequence Label: pSTTa-GNAO1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggatgta ctctgagcgc agaggagaga gccgccctcg agcggagcaa ggcgattgag
       61 aaaaacctca aagaggatgg catcagcgcc gccaaagacg tgaaattact cctgctcggg
      121 gctggagaat caggaaaaag caccattgtg aagcagatga agatcatcca tgaagatggc
      181 ttctccggag aagacgtgaa acagtacaag cctgttgtct acagcaacac tatccagtcc
      241 ctggcagcca tcgtccgggc catggacact ttgggcatcg aatatggtga taaggagaga
      301 aaggctgacg ccaagatggt gtgtgatgtg gtgagtcgga tggaagacac cgagcccttc
      361 tctgcagagc tgctttctgc catgatgcgg ctctggggcg actcaggaat ccaagagtgc
      421 ttcaaccggt cccgggagta tcagctcaac gactctgcca aatactacct ggacagcctg
      481 gatcggattg gggccgccga ctaccagccc accgagcagg acatcctccg aaccagggtc
      541 aaaaccactg gcatcgtaga aacccacttc acattcaaga acctccactt caggctgttt
      601 gacgtcggag gccagcgatc tgaacgcaag aagtggatcc attgcttcga ggacgtcacg
      661 gccatcattt tctgtgtcgc gctcagcggc tatgaccagg tgctccacga agacgaaacc
      721 acgaaccgca tgcacgagtc tctcatgctc ttcgactcca tctgtaacaa caagttcttc
      781 atcgatacct ccatcattct cttcctcaac aagaaagatc tctttggcga gaagatcaag
      841 aagtcacctt tgaccatctg ctttcctgaa tacacaggcc ccaataccta tgaagacgca
      901 gccgcctaca tccaagcaca atttgaaagc aaaaaccgct cacccaacaa agaaatatat
      961 tgtcacatga cttgtgccac agacacgaat aacatccagg tggtgttcga cgccgtcacc
     1021 gacatcatca ttgccaacaa cctccggggc tgcggcttgt actga
//
